Strain Name:

B6;129S6-Rr23tm1Axvi/Mmjax

Availability:

Cryopreserved - Ready for recovery     Available at the JAX MMRRC

Common Names: Enhancer HS746KO;    
Please refer to the Mutant Mouse Regional Resource Center (MMRRC) for information about B6;129S6-Rr23tm1Axvi/Mmjax MMRRC Stock Number 036888.
In this knockout of the non-coding enhancer element (Rr23or HS746), mRNA expression of Msx1 in mouse embryos is diminished and adult skulls exhibit significant differences in skull morphology. This strain may be useful for downstream analysis of craniofacial development.

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Mutant Stock; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
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Mating SystemHomozygote x Homozygote         (Female x Male)   19-SEP-13
Specieslaboratory mouse
 
Donating Investigator Axel Visel,   Lawrence Berkeley National Laboratory

Description
The regulatory region 23 (Rr23 or HS746) is a non-coding enhancer element that controls expression of Msx1 (homeobox, msh-like 1), a gene involved in craniofacial development. qPCR analysis indicates a 1.5 fold down regulation of Msx1 in homozygous HS1431 E11.5 and E13.5 embryos. Analysis of the adult skulls from homozygous mice by micro-computed tomography reveals significant differences in skull morphology compared to wild type. Alterations in HS746 include a shortening of the face, a widening of the posterior neurocranium, a narrowing of the palate and shortening of the cranial base. This strain may be useful for downstream analysis of craniofacial development.

Development
A targeting vector containing a loxP-flanked PGKneo cassette was used to delete 1682 bp of the HS746 enhancer sequence. Transient Cre expression in targeted cells excised the neo cassette. The construct was electroporated into 129S6/SvEvTac-derived W4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice for approximately three generations. Upon arrival, mice were bred to C57BL/6J for at least 1 generation to establish the colony.

Control Information

  Control
   101045 B6129SF2/J (approximate)
 
  Considerations for Choosing Controls

Related Strains

Facebase: models
007664   129S-Efnb1tm1Sor/J
000646   A/J
000647   A/WySnJ
005709   B6.129-Skitm1Cco/J
002619   B6.129-Tgfb3tm1Doe/J
007453   B6.129P2(Cg)-Dhcr7tm1Gst/J
010525   B6.129S-Notch2tm3Grid/J
010616   B6.129S1-Jag1tm1Grid/J
010546   B6.129S1-Jag2tm1Grid/J
010620   B6.129S1-Notch2tm1Grid/J
009387   B6.129S1-Osr1tm1Jian/J
009386   B6.129S1-Osr2tm1Jian/J
010621   B6.129S1-Snai1tm2.1Grid/J
010617   B6.129S1-Snai2tm1Grid/J
003865   B6.129S2-Itgavtm1Hyn/J
003755   B6.129S4-Meox2tm1(cre)Sor/J
016902   B6.129S5-Irf6Gt(OST398253)Lex/J
003336   B6.129S7-Cdkn1ctm1Sje/J
012843   B6.129X1(Cg)-Slc32a1tm1.1Bgc/J
000026   B6.C3-Gli3Xt-J/J
004275   B6.Cg-Fignfi/Frk
012844   B6.Cg-Gad1tm1.1Bgc/J
006382   B6;129-Casktm1Sud/J
002711   B6;129-Gabrb3tm1Geh/J
004293   B6;129-Shhtm2Amc/J
012603   B6;129-Tgfbr2tm1Karl/J
010618   B6;129S-Jag1tm2Grid/J
010686   B6;129S-Snai1tm2Grid/J
009389   B6;129S1-Bambitm1Jian/J
010619   B6;129S1-Lfngtm1Grid/J
010547   B6;129S1-Notch3tm1Grid/J
010544   B6;129S1-Notch4tm1Grid/J
010722   B6;129S1-Snai2tm2Grid/J
012463   B6;129S4-Foxd1tm1(GFP/cre)Amc/J
022359   B6;129S6-Rr24tm1Axvi/Mmjax
022360   B6;129S6-Rr25tm1Axvi/Mmjax
003277   B6;129S7-Acvr2atm1Zuk/J
002788   B6;129S7-Fsttm1Zuk/J
002990   B6;129S7-Inhbatm1Zuk/J
000523   B6By.Cg-Eh/J
000278   B6C3Fe a/a-Papss2bm Hps1ep Hps6ru/J
000515   B6CBACa Aw-J/A-SfnEr/J
001434   C3HeB/FeJ x STX/Le-Mc1rE-so Gli3Xt-J Zeb1Tw/J
000252   DC/LeJ
005057   FVB.129-Kcnj2tm1Swz/J
012655   FVB.A-Irf6clft1/BeiJ
013100   FVB.C-Prdm16csp1/J
017437   FVB/N-Ckap5TgTn(sb-cHS4,Tyr)2320F-1Ove/J
017438   FVB/N-MidnTg(Tyr)2261EOve/J
017609   FVB/N-Rr16Tn(sb-Tyr)1HCebOve/J
017598   FVB/N-Sdccag8Tn(sb-Tyr)2161B.CA1C2Ove/J
017608   FVB/N-Skor2Tn(sb-Tyr)1799B.CA7BOve/J
017436   FVB/N-Tapt1TgTn(sb-cHS4,Tyr)2508GOve/J
016870   FVB/NJ-Ap2b1Tg(Tyr)427Ove/EtevJ
017434   FVB;B6-Cramp1lTgTn(sb-rtTA,Tyr)2447AOve/J
017594   FVB;B6-Eya4TgTn(Prm1-sb10,sb-Tyr)1739AOve/J
017435   FVB;B6-SlmapTn(sb-rtTA)2426B.SB4Ove/J
003318   STOCK Shhtm1Amc/J
003102   STOCK Tgfb2tm1Doe/J
018624   STOCK Tgfb3tm2(Tgfb1)Vk/J
008469   STOCK Wnt9btm1.2Amc/J
View Facebase: models     (61 strains)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Rr23tm1Axvi/Rr23tm1Axvi

        involves: 129S6/SvEvTac * C57BL/6J
  • craniofacial phenotype
  • abnormal cranium morphology
    • micro-computed tomography analysis reveals significant differences in skull morphology compared to wild type   (MGI Ref ID J:199212)
    • abnormal basicranium morphology
      • shortening of the cranial base   (MGI Ref ID J:201949)
    • abnormal neurocranium morphology
      • increase in the width of the posterior neurocranium   (MGI Ref ID J:201949)
  • abnormal facial morphology
    • decrease of facial length   (MGI Ref ID J:201949)
    • narrow palate   (MGI Ref ID J:201949)
  • skeleton phenotype
  • abnormal cranium morphology
    • micro-computed tomography analysis reveals significant differences in skull morphology compared to wild type   (MGI Ref ID J:199212)
    • abnormal basicranium morphology
      • shortening of the cranial base   (MGI Ref ID J:201949)
    • abnormal neurocranium morphology
      • increase in the width of the posterior neurocranium   (MGI Ref ID J:201949)
  • digestive/alimentary phenotype
  • narrow palate   (MGI Ref ID J:201949)
  • growth/size/body phenotype
  • abnormal facial morphology
    • decrease of facial length   (MGI Ref ID J:201949)
    • narrow palate   (MGI Ref ID J:201949)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Developmental Biology Research
Craniofacial and Palate Defects

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Rr23tm1Axvi
Allele Name targeted mutation 1, Axel Visel
Allele Type Targeted (Null/Knockout)
Common Name(s) HS746_;
Strain of Origin129S6/SvEvTac
Gene Symbol and Name Rr23, regulatory region 23
Chromosome 5
Gene Common Name(s) HS746;
Molecular Note The enhancer sequence was replaced with a floxed neo selection cassette which was subsequently removed by cre excision. [MGI Ref ID J:199212]

Genotyping

Genotyping Information

Genotyping Protocols

Rr23tm1AxviAlternate 1, Separated PCR
Rr24tm1Axvialternate1, Separated PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Additional References

Rr23tm1Axvi related

Attanasio C; Nord AS; Zhu Y; Blow MJ; Li Z; Liberton DK; Morrison H; Plajzer-Frick I; Holt A; Hosseini R; Phouanenavong S; Akiyama JA; Shoukry M; Afzal V; Rubin EM; FitzPatrick DR; Ren B; Hallgrimsson B; Pennacchio LA; Visel A. 2013. Fine tuning of craniofacial morphology by distant-acting enhancers. Science 342(6157):1241006. [PubMed: 24159046]  [MGI Ref ID J:201949]

Attanasio C; Visel A. 2013. Distant-Acting Craniofacial Enhancers MGI Direct Data Submission :.  [MGI Ref ID J:199212]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryWhile maintaining a live colony, these mice are bred as homozygotes.
Mating SystemHomozygote x Homozygote         (Female x Male)   19-SEP-13

Pricing and Purchasing

Supply Notes


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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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