|Please refer to the Mutant Mouse Regional Resource Center (MMRRC) for information about FVB;129S6-Astltm1Dean/Mmjax MMRRC Stock Number 036900.|
|Female mice homozygous for the null Astl or ovastacin (astacin-like metalloendopeptidase (M12 family)) allele exhibit moderately reduced fecundity. This strain may be useful for studying post-fertilization sperm blocking, and sperm/oocyte interaction during fertilization and early development.|
Type Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Mating System Heterozygote x Homozygote (Female x Male) 19-SEP-13 Species laboratory mouse Donating Investigator Dr. Jurrien Dean, NIH
Astl or ovastacin (astacin-like metalloendopeptidase (M12 family)) encodes an oocyte-specific metalloendoprotease involved in cleavage of the zona pellucida protein ZP2. Cleavage of ZP2 modifies the zona pellucida by destroying the sperm binding domain preventing post-fertilization binding and polyspermy. In the absence of ovastacin, sperm binds to two-cell Astl-null embryos. Homozygous mice are viable and fertile, although female mice exhibit moderately reduced fecundity. This strain may be useful for studying post-fertilization sperm blocking, sperm/oocyte interaction during fertilization and early development.
A targeting vector containing the neomycin resistance gene was used to replace exons 2-3, thus deleting the transcriptional and translation start sites. The construct was electroporated into 129S6/SvEvTac-derived TC-1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts. The resulting chimeric animals were crossed to FVB female mice. Upon arrival, mice were bred to FVB/NJ for at least 1 generation to establish the colony.
|Wild-type from the colony||(approximate)|
|Considerations for Choosing Controls|
View Mammalian Phenotype TermsMammalian Phenotype Terms provided by MGIassigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Astltm1Dean/Astltm1Deaninvolves: 129S6/SvEvTacView Research ApplicationsResearch ApplicationsThis mouse can be used to support research in many areas including:
Reproductive Biology Research
Developmental Defects Affecting Gonads
|Allele Name||targeted mutation 1, Jurrien Dean|
|Allele Type||Targeted (knock-out)|
|Strain of Origin||129S6/SvEvTac|
|Gene Symbol and Name||Astl, astacin-like metalloendopeptidase (M12 family)|
|Gene Common Name(s)||C87576; MGC:76457; Ovastacin; RGD1562279; Sas1b; expressed sequence C87576;|
|Molecular Note||Exons 2 and 3 of the gene, containing the transcriptional and translational start sites, were replaced by a neomycin resistance marker. [MGI Ref ID J:185161]|
Animal Health ReportsProduction of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.
Breeding & Husbandry Female mice exhibit moderately reduced fecundity. The donating investigator maintains this strain by heterozygote female x homozygote male or heterozygote x heterozygote matings. Mating System Heterozygote x Homozygote (Female x Male) 19-SEP-13
MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.
In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.
In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.
MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.
The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.
Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.