|Please refer to the Mutant Mouse Regional Resource Center (MMRRC) for information about FVB;129S6-Astltm1Dean/Mmjax MMRRC Stock Number 036900.|
|Female mice homozygous for the null Astl or ovastacin (astacin-like metalloendopeptidase (M12 family)) allele exhibit moderately reduced fecundity. This strain may be useful for studying post-fertilization sperm blocking, and sperm/oocyte interaction during fertilization and early development.|
Type Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Mating System Heterozygote x Homozygote (Female x Male) 19-SEP-13 Species laboratory mouse Donating Investigator Dr. Jurrien Dean, NIH
Astl or ovastacin (astacin-like metalloendopeptidase (M12 family)) encodes an oocyte-specific metalloendoprotease involved in cleavage of the zona pellucida protein ZP2. Cleavage of ZP2 modifies the zona pellucida by destroying the sperm binding domain preventing post-fertilization binding and polyspermy. In the absence of ovastacin, sperm binds to two-cell Astl-null embryos. Homozygous mice are viable and fertile, although female mice exhibit moderately reduced fecundity. This strain may be useful for studying post-fertilization sperm blocking, sperm/oocyte interaction during fertilization and early development.
A targeting vector containing the neomycin resistance gene was used to replace exons 2-3, thus deleting the transcriptional and translation start sites. The construct was electroporated into 129S6/SvEvTac-derived TC-1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts. The resulting chimeric animals were crossed to FVB female mice. Upon arrival, mice were bred to FVB/NJ for at least 1 generation to establish the colony.
|Wild-type from the colony||(approximate)|
|Considerations for Choosing Controls|
View Mammalian Phenotype TermsMammalian Phenotype Terms provided by MGIassigned by genotype
The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.
Astltm1Dean/Astltm1Deaninvolves: 129S6/SvEvTacView Research ApplicationsResearch ApplicationsThis mouse can be used to support research in many areas including:
Reproductive Biology Research
Developmental Defects Affecting Gonads
|Allele Name||targeted mutation 1, Jurrien Dean|
|Allele Type||Targeted (Null/Knockout)|
|Strain of Origin||129S6/SvEvTac|
|Gene Symbol and Name||Astl, astacin-like metalloendopeptidase (M12 family)|
|Gene Common Name(s)||C87576; MGC:76457; Ovastacin; RGD1562279; SAS1B; expressed sequence C87576;|
|Molecular Note||Exons 2 and 3 of the gene, containing the transcriptional and translational start sites, were replaced by a neomycin resistance marker. [MGI Ref ID J:185161]|
Animal Health ReportsProduction of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.
Breeding & Husbandry Female mice exhibit moderately reduced fecundity. The donating investigator maintains this strain by heterozygote female x homozygote male or heterozygote x heterozygote matings. Mating System Heterozygote x Homozygote (Female x Male) 19-SEP-13
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