Strain Name:

B6.Cg-Hdac2tm1.1Rdp/J

Stock Number:

022625

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Availability:

Under Development - Now Accepting Orders

Estimated Available for Distribution Date: 23-JUN-14
Use Restrictions Apply, see Terms of Use
These mice carry a floxed allele of the Hdac2 (histone deacetylase 2) gene. Exons 5 and 6, encoding the HDAC-catalytic core of the protein, are flanked by loxP sites. Cre-mediated excision of the floxed segment results in a null genotype, lacking protein expression. HDAC2 deficiency results in increased synapse number and memory facilitation.

Description

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Mating SystemHeterozygote x Heterozygote         (Female x Male)   06-NOV-13
Specieslaboratory mouse
GenerationN8+pN1 (24-JAN-14)
Generation Definitions
 
Donating Investigator Li-Huei Tsai,   Massachusetts Institue of Technology
Donating InvestigatorDr. Ronald DePinho,   MD Anderson Cancer Center

Description
Chromatin remodeling, especially through histone-tail acetylation, is emerging as a fundamental mechanism for regulating gene expression. Inhibition of histone deacetylases increases histone-tail acetylation and facilitates learning and memory in wildtype mice as well as in mouse models of neurodegeneration. HDAC2 (histone deacetylase 2) deficiency results in increased synapse number and memory facilitation.

These mice carry a floxed allele of the Hdac2 gene. Exons 5 and 6, encoding the HDAC-catalytic core of the protein, are flanked by loxP sites. Western blot analysis demonstrates that HDAC2 expression in floxed mice is similar to that of wildtype. Cre-mediated excision of the floxed segment results in a null genotype, lacking protein expression.

Deletion of the protein in the germline using EIIa-cre or nestin-cre transgenic mice results in viable and fertile heterozyous mice with no obvious histological abnormalities up to a year of age. Homozygous knockout mice are born at a frequency that is 2-fold lower than predicted by Mendelian ratios and have compromised fertility. Both male and female homozygous knockout mice are approximately 25% smaller than wildtype and heterozygous littermates.

Development
Exons 5 and 6 were flanked by loxP sites in (C57BL/6 x 129S4/SvJae)F1-derived V6.5 embryonic stem (ES) cells. The targeting construct contained a floxed neomycin cassette which was removed by transient Cre expression. Resultant mice were established on an FVB genetic background then backcrossed to C57BL/6 for 8 generations by the donating lab.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Hdac2
012639   B6;129S4-Mapttm3(HDAC2)Jae/J
012640   STOCK Hdac2tm1.2Rdp/J
View Strains carrying other alleles of Hdac2     (2 strains)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Hdac2tm1.1Rdp/Hdac2tm1.1Rdp

        involves: 129S4/SvJae * C57BL/6 * FVB/N
  • no phenotypic analysis
  • *normal* no phenotypic analysis   (MGI Ref ID J:148391)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Neurobiology Research
Behavioral and Learning Defects
Cre-lox System
      loxP-flanked Sequences

Research Tools
Cre-lox System
      loxP-flanked Sequences
Developmental Biology Research
      Cre-lox System
Neurobiology Research

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Hdac2tm1.1Rdp
Allele Name targeted mutation 1.1, Ronald DePinho
Allele Type Targeted (Floxed/Frt)
Common Name(s) Hdac2L;
Strain of Origin(C57BL/6 x 129S4/SvJae)F1
Gene Symbol and Name Hdac2, histone deacetylase 2
Chromosome 10
Gene Common Name(s) D10Wsu179e; DNA segment, Chr 10, Wayne State University 179, expressed; HD2; RPD3; YAF1; YY1 transcription factor binding protein; Yy1bp;
Molecular Note LoxP sites were inserted upstream of exon 5 and downstream of exon 6. Protein expression was unaffected as determined by immunoblot analysis of embryonic fibroblasts. Cre-mediated recombination removed the drug selection cassette. [MGI Ref ID J:148391]

Genotyping

Genotyping Information

Genotyping Protocols

Hdac2tm1.1Rdp, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Guan JS; Haggarty SJ; Giacometti E; Dannenberg JH; Joseph N; Gao J; Nieland TJ; Zhou Y; Wang X; Mazitschek R; Bradner JE; DePinho RA; Jaenisch R; Tsai LH. 2009. HDAC2 negatively regulates memory formation and synaptic plasticity. Nature 459(7243):55-60. [PubMed: 19424149]  [MGI Ref ID J:148391]

Additional References

Hdac2tm1.1Rdp related

Heideman MR; Wilting RH; Yanover E; Velds A; de Jong J; Kerkhoven RM; Jacobs H; Wessels LF; Dannenberg JH. 2013. Dosage-dependent tumor suppression by histone deacetylases 1 and 2 through regulation of c-Myc collaborating genes and p53 function. Blood 121(11):2038-50. [PubMed: 23327920]  [MGI Ref ID J:195869]

Wilting RH; Yanover E; Heideman MR; Jacobs H; Horner J; van der Torre J; DePinho RA; Dannenberg JH. 2010. Overlapping functions of Hdac1 and Hdac2 in cell cycle regulation and haematopoiesis. EMBO J 29(15):2586-97. [PubMed: 20571512]  [MGI Ref ID J:197818]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX18

Colony Maintenance

Breeding & HusbandryHeterozygous and homozygous floxed mice are viable and fertile.
Mating SystemHeterozygote x Heterozygote         (Female x Male)   06-NOV-13

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


 

This strain is currently Under Development - Now Accepting Orders.
Estimated Available for Distribution Date: 23-JUN-14

Please note: You may now place orders for this strain although it is not yet ready for distribution. Estimated available for distribution dates are provided to keep customers better informed on strains under development. Please note that our Colony Managers routinely monitor the target date and edit it based on breeding performance and other factors. The length of time it takes to make a new strain available for distribution depends on genotype, age, number of animals sent by the Donating Investigator, breeding performance, additional strain development (backcrossing, making homozygous), and anticipated demand for the strain.

Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $232.00Female or MaleHeterozygous for Hdac2tm1.1Rdp  
$232.00Female or MaleHomozygous for Hdac2tm1.1Rdp  
Price per Pair (US dollars $)Pair Genotype
$464.00Heterozygous for Hdac2tm1.1Rdp x Heterozygous for Hdac2tm1.1Rdp  

Standard Supply

Under Development - Now Accepting Orders The strain development process (i.e. importation, rederivation, and colony expansion) usually takes six to nine months.

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $301.60Female or MaleHeterozygous for Hdac2tm1.1Rdp  
$301.60Female or MaleHomozygous for Hdac2tm1.1Rdp  
Price per Pair (US dollars $)Pair Genotype
$603.20Heterozygous for Hdac2tm1.1Rdp x Heterozygous for Hdac2tm1.1Rdp  

Standard Supply

Under Development - Now Accepting Orders The strain development process (i.e. importation, rederivation, and colony expansion) usually takes six to nine months.

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Under Development - Now Accepting Orders The strain development process (i.e. importation, rederivation, and colony expansion) usually takes six to nine months.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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