Strain Name:

B6;129-Zic3tm2.1Jwb/J

Stock Number:

023162

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Availability:

Under Development - Now Accepting Orders

Estimated Available for Distribution Date: 30-JUN-14
Use Restrictions Apply, see Terms of Use
These floxed mutant mice possess loxP sites flanking exon 1 of the X-linked Zic3 gene and may be useful in applications related to the study of embryonic development.

Description

Strain Information

Type Mutant Stock; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
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Mating SystemWild-type x Hemizygote         (Female x Male)   02-OCT-13
Mating SystemHeterozygote x Wild-type         (Female x Male)   02-OCT-13
Specieslaboratory mouse
Generation?+pN1 (11-NOV-13)
Generation Definitions
 
Donating Investigator John Belmont,   Baylor College of Medicine

Description
These Zic3flox mice possess loxP sites flanking exon 1 of the X-linked zinc finger protein of the cerebellum 3 (Zic3) gene. ZIC3 is a transcription factor involved in early stages of gastrulation, and central nervous system, cardiac and left-right axial development. Zic3flox/flox females and Zic3flox/y males are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting double mutant offspring will have exon 1 deleted in the cre-expressing tissue(s).

For example, when bred to B6.Cg-Tg(Sox2-cre)1Amc/J transgenic mice (Stock No. 008454) expressing cre in epiblast cells, resulting offspring exhibit 'kinked' tails, anus and eye anomalies, cardiac looping defects, cleft palate, exencephaly, abnormal lung position, and axial skeleton defects leading to hind-limb paralysis.

Development
A targeting vector was designed to insert a frt-flanked PGK-neo cassette followed by a loxP site upstream of exon 1, and a second loxP site downstream of exon 1 of the X-linked zinc finger protein of the cerebellum 3 (Zic3) gene. The construct was electroporated into 129S7/SvEvBrd-Hprtb-m2-derived AB2.2 embryonic stem (ES) cells. Correctly targeted ES cells were injected into B6(Cg)-Tyrc-2J/J blastocysts and resulting chimeric males were bred to B6(Cg)-Tyrc-2J/J females (Stock No. 000058). Heterozygous females were crossed to FLPe males on a 129/Sv background to remove the neo cassette, and offspring were crossed to remove the Flp-expressing transgene. Zic3flox/flox females and Zic3flox/y males were maintained on a mixed background. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J (Stock No. 000664) for at least one generation and the Tyrc-2J allele was selected against.

Control Information

  Control
   101045 B6129SF2/J (approximate)
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Zic3
000250   BNT/LeJ
View Strains carrying other alleles of Zic3     (1 strain)

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested.
Heterotaxy, Visceral, 1, X-Linked; HTX1   (ZIC3)
Vacterl Association, X-Linked, with or without Hydrocephalus; VACTERLX   (ZIC3)
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Zic3tm2.1Jwb/Zic3tm2.1Jwb

        involves: 129S4/SvJaeSor * 129S7/SvSvBrd * C57BL/6J
  • normal phenotype
  • no abnormal phenotype detected
    • mice are viable and fertile and phenotypically indistinguishable from the wild-type mice   (MGI Ref ID J:192577)

Zic3tm2.1Jwb/Y

        involves: 129S4/SvJaeSor * 129S7/SvSvBrd * C57BL/6J
  • normal phenotype
  • no abnormal phenotype detected
    • mice are viable and fertile and phenotypically indistinguishable from the wild-type mice   (MGI Ref ID J:192577)

The following phenotype relates to a compound genotype created using this strain.
Contact JAX® Services jaxservices@jax.org for customized breeding options.

Zic3tm2.1Jwb/Y Tg(Sox2-cre)1Amc/0

        involves: 129S4/SvJaeSor * 129S7/SvSvBrd * C57BL/6 * CBA   (conditional)
  • mortality/aging
  • partial lethality throughout fetal growth and development
    • despite normal numbers at E15.5, fewer than expected mice at weaning   (MGI Ref ID J:192577)
  • cardiovascular system phenotype
  • abnormal heart looping
    • in 5 of 32 mice at E9.5   (MGI Ref ID J:192577)
    • abnormal direction of heart looping
      • sinisteral looping   (MGI Ref ID J:192577)
  • abnormal heart position or orientation
    • atrium isomerism in some mice   (MGI Ref ID J:192577)
    • dextrocardia
  • abnormal inferior vena cava morphology
    • separate entrance of hepatic veins in some mice   (MGI Ref ID J:192577)
  • atrial septal defect
  • ventricular septal defect
  • digestive/alimentary phenotype
  • abnormal anus morphology
  • abnormal stomach position or orientation
    • midline and right-sided stomach in some mice   (MGI Ref ID J:192577)
    • right-sided stomach
  • cleft palate
    • in 4 of 38 mice at E15.5   (MGI Ref ID J:192577)
  • embryogenesis phenotype
  • abnormal embryogenesis/ development
    • in some mice at E9.5   (MGI Ref ID J:192577)
    • abnormal neural tube morphology/development
      • in 4 of 38 mice at E15.5   (MGI Ref ID J:192577)
    • embryonic growth retardation
      • in 14 of 32 mice   (MGI Ref ID J:192577)
  • growth/size/body phenotype
  • embryonic growth retardation
    • in 14 of 32 mice   (MGI Ref ID J:192577)
  • heterotaxia
    • laterality defects in 2 of 8 mice   (MGI Ref ID J:192577)
    • right-sided stomach
  • nervous system phenotype
  • abnormal neural tube morphology/development
    • in 4 of 38 mice at E15.5   (MGI Ref ID J:192577)
  • exencephaly
    • in 4 of 38 mice at E15.5   (MGI Ref ID J:192577)
  • behavior/neurological phenotype
  • hindlimb paralysis
  • vision/eye phenotype
  • abnormal eye morphology
  • skeleton phenotype
  • abnormal axial skeleton morphology
  • respiratory system phenotype
  • abnormal lung position or orientation
    • pulmonary isomerism in some mice   (MGI Ref ID J:192577)
  • limbs/digits/tail phenotype
  • kinked tail   (MGI Ref ID J:192577)
  • craniofacial phenotype
  • cleft palate
    • in 4 of 38 mice at E15.5   (MGI Ref ID J:192577)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Cardiovascular Research

Developmental Biology Research
Limb Patterning Defects
Neural Tube Defects

Neurobiology Research
Cre-lox System
      loxP-flanked Sequences

Research Tools
Cre-lox System
      loxP-flanked Sequences

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Zic3tm2.1Jwb
Allele Name targeted mutation 2.1, John W Belmont
Allele Type Targeted (Floxed/Frt)
Common Name(s) Zic3flox;
Strain of Origin129S7/SvEvBrd-Hprt
Gene Symbol and Name Zic3, zinc finger protein of the cerebellum 3
Chromosome X
Gene Common Name(s) Bn; HTX; HTX1; Ka; VACTERLX; ZNF203; bent tail; katun;
Molecular Note An FRT-flanked neo cassette with a 3' loxP site was inserted upstream of exon 1. An additional loxP site was inserted downstream of exon 1. Flp-mediated recombination removed the neo cassette and left exon 1 floxed. [MGI Ref ID J:192577]

Genotyping

Genotyping Information

Genotyping Protocols

Zic3tm2.1Jwb, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Jiang Z; Zhu L; Hu L; Slesnick TC; Pautler RG; Justice MJ; Belmont JW. 2013. Zic3 is required in the extra-cardiac perinodal region of the lateral plate mesoderm for left-right patterning and heart development. Hum Mol Genet 22(5):879-89. [PubMed: 23184148]  [MGI Ref ID J:192577]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           FGB27

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, homozygous mice may be bred together.
Mating SystemWild-type x Hemizygote         (Female x Male)   02-OCT-13
Heterozygote x Wild-type         (Female x Male)   02-OCT-13
Diet Information LabDiet® 5K52/5K67

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


 

This strain is currently Under Development - Now Accepting Orders.
Estimated Available for Distribution Date: 30-JUN-14

Please note: You may now place orders for this strain although it is not yet ready for distribution. Estimated available for distribution dates are provided to keep customers better informed on strains under development. Please note that our Colony Managers routinely monitor the target date and edit it based on breeding performance and other factors. The length of time it takes to make a new strain available for distribution depends on genotype, age, number of animals sent by the Donating Investigator, breeding performance, additional strain development (backcrossing, making homozygous), and anticipated demand for the strain.

Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $232.00MaleHemizygous for Zic3tm2.1Jwb  
$232.00FemaleHeterozygous for Zic3tm2.1Jwb  
Individual Mouse $232.00FemaleHomozygous for Zic3tm2.1Jwb  
Price per Pair (US dollars $)Pair Genotype
$464.00Heterozygous for Zic3tm2.1Jwb x Hemizygous for Zic3tm2.1Jwb  

Standard Supply

Under Development - Now Accepting Orders The strain development process (i.e. importation, rederivation, and colony expansion) usually takes six to nine months.

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $301.60MaleHemizygous for Zic3tm2.1Jwb  
$301.60FemaleHeterozygous for Zic3tm2.1Jwb  
Individual Mouse $301.60FemaleHomozygous for Zic3tm2.1Jwb  
Price per Pair (US dollars $)Pair Genotype
$603.20Heterozygous for Zic3tm2.1Jwb x Hemizygous for Zic3tm2.1Jwb  

Standard Supply

Under Development - Now Accepting Orders The strain development process (i.e. importation, rederivation, and colony expansion) usually takes six to nine months.

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Under Development - Now Accepting Orders The strain development process (i.e. importation, rederivation, and colony expansion) usually takes six to nine months.

Control Information

  Control
   101045 B6129SF2/J (approximate)
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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