Strain Name:

B6.129(Cg)-Homer2tm1Mhd/PfwJ

Stock Number:

023313

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Availability:

Repository- Live

These Homer2 KO mice may be useful for studying the role of HOMER2 on neurochemical defects.

Description

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Mating SystemHeterozygote x Heterozygote         (Female x Male)   14-FEB-14
Specieslaboratory mouse
GenerationN5+pN1 (02-MAY-14)
Generation Definitions
 
Donating Investigator Paul Worley,   Johns Hopkins University School of Medic

Description
These Homer2 KO mice have a neomycin cassette inserted into exon 3 of homer homolog 2 (Homer2) gene, abolishing gene expression. HOMER2 is a postsynaptic density scaffolding protein expressed in the central nervous system, as well as skeletal muscle, where it mediates the formation of large macromolecular complexes and regulates group 1 metabotrophic glutamate receptor function. Mice that are homozygous for this allele are viable and fertile. These mice avoid drinking high concentrations of ethanol (EtOH) and do not develop place preference or locomotor sensitization after administration. They display an increase in sensitivity to cocaine-induced locomotion and conditioned reward. They do not display any abnormalities in cognitive, motivational or emotional processing.

Development
A targeting vector was designed to insert a neomycin resistance (neo) cassette, in reverse orientation, into exon 3 of the homer homolog 2 (Homer2) gene. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-Kitl+-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and the resulting chimeric males were bred to C57BL/6J females. The donating investigator reports that these mutant mice were then backcrossed to C57BL/6J mice for at least 5 generations (see SNP note below). Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J (Stock No. 000664) for at least one generation to establish the colony.

A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, all 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a C57BL/6N genetic background.

Control Information

  Control
   000664 C57BL/6J (approximate)
 
  Considerations for Choosing Controls

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Homer2tm1Mhd/Homer2tm1Mhd

        B6.129-Homer2tm1Mhd
  • behavior/neurological phenotype
  • *normal* behavior/neurological phenotype   (MGI Ref ID J:96554)
    • abnormal associative learning
      • mutants exhibit cocaine-induced place conditioning compared to wild-type controls   (MGI Ref ID J:96554)
      • mice exhibit cocaine-induced place conditioning (increased time spent in environment containing stimulus/reward) at doses that do not elicit place conditioning in controls   (MGI Ref ID J:96554)
      • mutants acquire operant self-administration of cocaine more readily than controls   (MGI Ref ID J:96554)
      • enhanced conditioned place preference behavior
        • mice prefer to spend more time in chamber where cocaine is available compared to controls   (MGI Ref ID J:96554)
    • abnormal cocaine consumption
      • mutants acquire operant self-administration of cocaine more readily than controls   (MGI Ref ID J:96554)
    • enhanced behavioral response to cocaine
      • capacity of acute cocaine to elicit locomotor hyperactivity is enhanced in mutants compared to controls   (MGI Ref ID J:96554)
  • nervous system phenotype
  • abnormal nervous system physiology
    • mutants show lower (~50%) extracellular glutamate levels in the nucleus accumbens compared to wild-type mice; injection of cocaine into the nucleus accumbens causes elevation of extracellular glutamate levels in mutants but not wild-type controls   (MGI Ref ID J:96554)

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Homer2tm1Mhd/Homer2tm1Mhd

        involves: 129S1/Sv * 129X1/SvJ * C57BL/6
  • endocrine/exocrine gland phenotype
  • abnormal pancreas physiology
    • carbachol stimulated acinar cells showed a higher increase in intracellular calcium concentration due to increased calcium release from internal stores than controls   (MGI Ref ID J:95213)
    • two-fold increased frequency of intracellular calcium oscillations in acinar cells in response to stimulation with bombesin or CCK compared to controls   (MGI Ref ID J:95213)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Neurobiology Research
Behavioral and Learning Defects
      genes regulating preferences to alcohol
Neurotransmitter Receptor and Synaptic Vesicle Defects

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Homer2tm1Mhd
Allele Name targeted mutation 1, Marlin H Dehoff
Allele Type Targeted (Null/Knockout)
Common Name(s) H2ko;
Strain of Origin(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
Gene Symbol and Name Homer2, homer homolog 2 (Drosophila)
Chromosome 7
Gene Common Name(s) 9330120H11Rik; ACPD; AW539445; CPD; Cupidin; HOMER-2; RIKEN cDNA 9330120H11 gene; VESL-2; expressed sequence AW539445;
Molecular Note A PGK-neomycin gene was inserted in inverse orientation into exon 3. mRNA and protein products could not be detected. [MGI Ref ID J:95213]

Genotyping

Genotyping Information

Genotyping Protocols

Generic Neo Melt Curve Analysis, Melt Curve Analysis
Homer2tm1Mhd-Alternate 1, Standard PCR
Homer3tm1Mhd-Alternate 1, Separated PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Shin DM; Dehoff M; Luo X; Kang SH; Tu J; Nayak SK; Ross EM; Worley PF; Muallem S. 2003. Homer 2 tunes G protein-coupled receptors stimulus intensity by regulating RGS proteins and PLCbeta GAP activities. J Cell Biol 162(2):293-303. [PubMed: 12860966]  [MGI Ref ID J:95213]

Additional References

Homer2tm1Mhd related

Hu JH; Yang L; Kammermeier PJ; Moore CG; Brakeman PR; Tu J; Yu S; Petralia RS; Li Z; Zhang PW; Park JM; Dong X; Xiao B; Worley PF. 2012. Preso1 dynamically regulates group I metabotropic glutamate receptors. Nat Neurosci 15(6):836-44. [PubMed: 22561452]  [MGI Ref ID J:191366]

Huang GN; Huso DL; Bouyain S; Tu J; McCorkell KA; May MJ; Zhu Y; Lutz M; Collins S; Dehoff M; Kang S; Whartenby K; Powell J; Leahy D; Worley PF. 2008. NFAT binding and regulation of T cell activation by the cytoplasmic scaffolding Homer proteins. Science 319(5862):476-81. [PubMed: 18218901]  [MGI Ref ID J:174501]

Kuwajima M; Dehoff MH; Furuichi T; Worley PF; Hall RA; Smith Y. 2007. Localization and expression of group I metabotropic glutamate receptors in the mouse striatum, globus pallidus, and subthalamic nucleus: regulatory effects of MPTP treatment and constitutive Homer deletion. J Neurosci 27(23):6249-60. [PubMed: 17553998]  [MGI Ref ID J:121962]

Park JM; Hu JH; Milshteyn A; Zhang PW; Moore CG; Park S; Datko MC; Domingo RD; Reyes CM; Wang XJ; Etzkorn FA; Xiao B; Szumlinski KK; Kern D; Linden DJ; Worley PF. 2013. A prolyl-isomerase mediates dopamine-dependent plasticity and cocaine motor sensitization. Cell 154(3):637-50. [PubMed: 23911326]  [MGI Ref ID J:200071]

Szumlinski KK; Dehoff MH; Kang SH; Frys KA; Lominac KD; Klugmann M; Rohrer J; Griffin W rd; Toda S; Champtiaux NP; Berry T; Tu JC; Shealy SE; During MJ; Middaugh LD; Worley PF; Kalivas PW. 2004. Homer proteins regulate sensitivity to cocaine. Neuron 43(3):401-13. [PubMed: 15294147]  [MGI Ref ID J:96554]

Szumlinski KK; Lominac KD; Frys KA; Middaugh LD. 2005. Genetic variation in heroin-induced changes in behaviour: effects of B6 strain dose on conditioned reward and locomotor sensitization in 129-B6 hybrid mice. Genes Brain Behav 4(5):324-36. [PubMed: 16011579]  [MGI Ref ID J:114613]

Szumlinski KK; Lominac KD; Kleschen MJ; Oleson EB; Dehoff MH; Schwarz MK; Seeburg PH; Worley PF; Kalivas PW. 2005. Behavioral and neurochemical phenotyping of Homer1 mutant mice: possible relevance to schizophrenia. Genes Brain Behav 4(5):273-88. [PubMed: 16011574]  [MGI Ref ID J:114304]

Szumlinski KK; Lominac KD; Oleson EB; Walker JK; Mason A; Dehoff MH; Klugman M; Cagle S; Welt K; During M; Worley PF; Middaugh LD; Kalivas PW. 2005. Homer2 is necessary for EtOH-induced neuroplasticity. J Neurosci 25(30):7054-61. [PubMed: 16049182]  [MGI Ref ID J:100023]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           FGB27

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, homozygous mice may be bred together.
Mating SystemHeterozygote x Heterozygote         (Female x Male)   14-FEB-14
Diet Information LabDiet® 5K52/5K67

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $232.00Female or MaleHeterozygous for Homer2tm1Mhd  
$232.00Female or MaleHomozygous for Homer2tm1Mhd  
Price per Pair (US dollars $)Pair Genotype
$464.00Heterozygous for Homer2tm1Mhd x Heterozygous for Homer2tm1Mhd  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $301.60Female or MaleHeterozygous for Homer2tm1Mhd  
$301.60Female or MaleHomozygous for Homer2tm1Mhd  
Price per Pair (US dollars $)Pair Genotype
$603.20Heterozygous for Homer2tm1Mhd x Heterozygous for Homer2tm1Mhd  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Control Information

  Control
   000664 C57BL/6J (approximate)
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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