Strain Name:

B6;129P2-Lcmt1Gt(CSC099)Byg/SclJ

Stock Number:

023332

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Availability:

Cryopreserved - Ready for recovery

Use Restrictions Apply, see Terms of Use
The Lcmt1Gt(CSC099)Byg hypomorphic allele has reduced LCMT1 protein expression. These mice may be useful in studying the role of LCMT1 and serine/threonine protein phosphatase methylation, as well as the biochemical and phenotypic effects of reduced LCMT1 activity.

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Gene Trap; Mutant Stock;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Specieslaboratory mouse
Generation+pN1
Generation Definitions
 
Donating Investigator Steven G Clarke,   University of California Los Angeles (UCLA)

Description
The Lcmt1Gt(CSC099)Byg hypomorphic allele has a β-geo gene trap cassette inserted into intron 1 of the leucine carboxyl methyltransferase 1 gene (Lcmt1). Due to splicing around the gene trap insertion, Lcmt1 transcript and LCMT1 protein levels are reduced but not eliminated. While Lcmt1 knockout mice are reported to exhibit full embryonic lethality, approximately 20% of mice homozygous for the Lcmt1 hypomorphic allele survive to birth. Homozygous mice (Lcmt1 hypomorphic mice) that survive development are viable and fertile with normal lifespan. Homozygotes have Lcmt1 expression affected in a tissue-dependent manner, with the largest decreases observed in cardiac and skeletal muscle, and smaller decreases in brain, liver, and kidney. Lcmt1 hypomorphic mice have decreased Lcmt1 methyltransferase activity and a concomitant decrease in serine/threonine protein phosphatase (PP2A) methylation. Homozygous mice also show decreased glucose tolerance and increased glucose-stimulated insulin secretion. The expected coat color is black or dark brown.

Development
The pGT0Lxf gene trap vector was designed with a mouse En2 intron-exon splice acceptor, a β-geo fusion reporter protein (β-galactosidase and neomycin phosphotransferase) and SV40 polyadenylation sequence. This gene trap vector was electroporated into 129P2/OlaHsd-derived E14TG2a embryonic stem (ES) cells. ES cells found to have a single gene trap vector randomly inserted into intron 1 of the leucine carboxyl methyltransferase 1 locus (Lcmt1) were identified and named line CSC099. Specifically, the gene trap cassette is located 19,455 nucleotides into intron 1 (or at position 19,729 of the gene [counting from the transcriptional start site in exon 1 and the first base of the initiating ATG codon designated as position 168]). Sequencing of the insertion site additionally revealed the insertion of 3 exogenous nucleotides at the 5prime end of the insertion site, as well as the truncation of 194 nucleotides from the 5prime end of the β-geo gene trap cassette. These CSC099 ES cells were injected into recipient blastocysts. Chimeric males were bred with C57BL/6 females to establish the colony. Mice heterozygous for the Lcmt1Gt(CSC099)Byg hypomorphic allele were backcrossed to C57BL/6 mice for at least three generations prior to sending to The Jackson Laboratory Repository in 2013. Upon arrival, males were used to cryopreserve sperm. To establish our living Lcmt1Gt(CSC099)Byg mouse colony, an aliquot of the frozen sperm was used to fertilize oocytes from C57BL/6J inbred females (Stock No. 000664).

Control Information

  Control
   Wild-type from the colony
   000664 C57BL/6J (approximate)
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Lcmt1
017425   FVB/N-Lcmt1TgTn(sb-cHS4,Tyr)2524BOve/Mmjax
View Strains carrying other alleles of Lcmt1     (1 strain)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Lcmt1Gt(CSC099)Byg/Lcmt1Gt(CSC099)Byg

        involves: 129P2/OlaHsd * C57BL/6
  • homeostasis/metabolism phenotype
  • impaired glucose tolerance   (MGI Ref ID J:201873)
  • increased circulating insulin level
    • glucose-stimulated   (MGI Ref ID J:201873)
  • insulin resistance
    • indicated by decreased glucose tolerance with higher insulin levels   (MGI Ref ID J:201873)
  • mortality/aging
  • partial embryonic lethality during organogenesis
    • many resorbing embryos are observed at E9.5   (MGI Ref ID J:201873)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Cancer Research
Growth Factors/Receptors/Cytokines

Cell Biology Research
Channel and Transporter Defects
Signal Transduction
Transcriptional Regulation

Developmental Biology Research
Embryonic Lethality (Homozygous)
      incomplete

Diabetes and Obesity Research
Insulin Receptors and Growth Factors

Immunology, Inflammation and Autoimmunity Research
Growth Factors/Receptors/Cytokines
Intracellular Signaling Molecules

Neurobiology Research
Channel and Transporter Defects
Receptor Defects

Research Tools
Genetics Research
      Mutagenesis and Transgenesis: transcriptional activation

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Lcmt1Gt(CSC099)Byg
Allele Name gene trap CSC099, BayGenomics
Allele Type Gene trapped
Strain of Origin129P2/OlaHsd
Gene Symbol and Name Lcmt1, leucine carboxyl methyltransferase 1
Chromosome 7
Gene Common Name(s) AL033290; LCMT; LCMT-1; Lcmt; MGC:11807; PPMT1; expressed sequence AL033290;
Molecular Note The beta-geo containing gene trap vector inserted into intron 1. This allele is hypomorphic. [MGI Ref ID J:201873]

Genotyping

Genotyping Information

Genotyping Protocols

Lcmt1Gt(CSC099)Byg, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

MacKay KB; Tu Y; Young SG; Clarke SG. 2013. Circumventing embryonic lethality with Lcmt1 deficiency: generation of hypomorphic Lcmt1 mice with reduced protein phosphatase 2A methyltransferase expression and defects in insulin signaling. PLoS One 8(6):e65967. [PubMed: 23840384]  [MGI Ref ID J:201873]

Nord AS; Chang PJ; Conklin BR; Cox AV; Harper CA; Hicks GG; Huang CC; Johns SJ; Kawamoto M; Liu S; Meng EC; Morris JH; Rossant J; Ruiz P; Skarnes WC; Soriano P; Stanford WL; Stryke D; von Melchner H; Wurst W; Yamamura K; Young SG; Babbitt PC; Ferrin TE. 2006. The International Gene Trap Consortium Website: a portal to all publicly available gene trap cell lines in mouse. Nucleic Acids Res 34(Database issue):D642-8. [PubMed: 16381950]  [MGI Ref ID J:201874]

Additional References

Lcmt1Gt(CSC099)Byg related

Mouse Genome Informatics (MGI) and National Center for Biotechnology Information (NCBI). 2008. Mouse Gene Trap Data Load from dbGSS Database Download :.  [MGI Ref ID J:141210]

Stryke D; Kawamoto M; Huang CC; Johns SJ; King LA; Harper CA; Meng EC; Lee RE; Yee A; L'Italien L; Chuang PT; Young SG; Skarnes WC; Babbitt PC; Ferrin TE. 2003. BayGenomics: a resource of insertional mutations in mouse embryonic stem cells. Nucleic Acids Res 31(1):278-81. [PubMed: 12520002]  [MGI Ref ID J:91812]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, heterozygous mice may be bred together. When breeding heterozygous mice together, approximately 20% of homozygotes survive to birth. Homozygous mice that survive development are viable and fertile with normal lifespan. The expected coat color is black or dark brown.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2140.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2782.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   Wild-type from the colony
   000664 C57BL/6J (approximate)
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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