Strain Name:

B6.Cg-Tg(HCRT-MJD)1Stak/J

Stock Number:

023418

Order this mouse

Availability:

Cryopreserved - Ready for recovery

Hypocretin (orexin) containing neurons are ablated in these transgenic mice, which may be useful in studies of narcolepsy, regulation of sleep/wakefulness, feeding behavior, and metabolic homeostasis.

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Congenic; Mutant Strain; Transgenic;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Specieslaboratory mouse
Generation+pN1
Generation Definitions
 
Donating Investigator Thomas Scammell,   Beth Israel Deaconess Medical Center

Description
These transgenic mice express a human ATXN3, ataxin 3, gene (a cDNA fragment encoding amino acid 286 to the C terminus, with 77 polyglutamine repeats, obtained from a patient with Machado-Joseph disease), under the control of the human HCRT, hypocretin (orexin) neuropeptide precursor, 3.2 kb upstream region, promoter. In transgenic mice, approximately 12 weeks of age, transgene expression results in the ablation of hypocretin (orexin) neurons (>99% loss). By 2 weeks of age, transgenic mice exhibit a loss of approximately half of hypocretin (orexin) containing neurons. Transgenic mice display reduced stress-induced hyperthermia, hypoactivity, and lower basal arterial blood pressure than wildtype controls. At approximately 6 weeks of age, transgenic mice display narcoleptic episodes consisting of a sudden stop to motor activity, postural changes, and ending with complete resumption of motor activity. While transgenic mice exhibit a level of sensitivity to anesthesia (induction of anesthesia) that is similar to wildtype controls, mutant mice have a delayed emergence from anesthesia (50% more time to emerge for both isoflurane and sevoflurane). No cataplectic episodes were detected during anesthesia experiments. Transgenic mice exhibit shorter retention of social recognition when compared to wildtype controls. Hippocampal synaptic plasticity is altered with both paired pulse facilitation (PPF) and long-term potentiation (LTP) are attenuated. Mice that are hemizygous for the targeted mutation are viable and fertile. The Donating Investigator has not attempted to make the strain homozygous.

Development
A transgenic insert designed by Dr. Takeshi Sakurai (University of Tsukuba) containing a human ATXN3, ataxin 3, gene (a cDNA fragment encoding amino acid 286 to the C terminus, with 77 polyglutamine repeats, obtained from a patient with Machado-Joseph disease), under the control of the 3.2 kb upstream region of the human HCRT, hypocretin (orexin) neuropeptide precursor, promoter, as well as a 5' HA epitope, 3' myc-tag, and a mouse protamine 1 (mP1) intron and poly(A) signal. The transgenic insert was injected into fertilized (C57BL/6 x DBA/1)F1 mouse eggs. Founder line 1 was subsequently established. The mice were then backcrossed to C57BL/6 for 15 generations by the Donating Investigator, Dr. Thomas Scammell (Beth Israel Deaconess Medical Center). The Donating Investigator has not attempted to make the strain homozygous. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of ATXN3
012705   B6;CBA-Tg(ATXN3*)84.2Cce/IbezJ
View Strains carrying other alleles of ATXN3     (1 strain)

Strains carrying other alleles of HCRT
009115   B6.129P2(Cg)-Hprttm15(Ple111-EGFP)Ems/Mmjax
009594   STOCK Hprttm32(Ple112-EGFP)Ems/Mmjax
View Strains carrying other alleles of HCRT     (2 strains)

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Potential model based on transgenic expression of an ortholog of a human gene that is associated with this disease. Phenotypic similarity to the human disease has not been tested.
Machado-Joseph Disease; MJD   (ATXN3)
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Tg(HCRT-MJD)1Stak/?

        B6.Cg-Tg(HCRT-MJD)1Stak
  • behavior/neurological phenotype
  • abnormal circadian feeding behavior
    • at night, mice consume less food than wild-type mice due to reduced duration of wake time   (MGI Ref ID J:141058)
  • abnormal drinking behavior
    • at night, mice consume less water than wild-type mice due to reduced duration of wake time   (MGI Ref ID J:141058)
    • initially, upon waking, mice consume more water than wild-type mice   (MGI Ref ID J:141058)
  • abnormal sleep pattern
    • mice exhibit more frequent short and fewer long episodes of wake and non-rapid eye movement (NREM) sleep compared to wild-type mice   (MGI Ref ID J:141058)
    • mice exhibit more REM sleep than wild-type mice during the night and direct transition from wake to REM sleep (DREM) unlike wild-type mice   (MGI Ref ID J:141058)
    • mice exhibit shorter transition from NREM to REM and more episodes of REM at night than wild-type mice   (MGI Ref ID J:141058)
  • decreased exploration in new environment
    • mice fail to exhibit as substantial of an increase in locomotor activity during dark phase as in wild-type mice when exposed to a novel environment   (MGI Ref ID J:141058)
  • hypoactivity
    • at night, mice exhibit decreased activity compared to wild-type mice due to reduced duration of wake time   (MGI Ref ID J:141058)
  • homeostasis/metabolism phenotype
  • abnormal body temperature
    • body temperature is higher than in wild-type between ZT14 and ZT18 and lower between ZT22 and ZT4 likely due to larger sleep-state dependent fluctuations in body temperature compared to in wild-type mice   (MGI Ref ID J:141058)
  • decreased energy expenditure
    • especially during the day   (MGI Ref ID J:141058)
  • growth/size/body phenotype
  • increased susceptibility to age related obesity
    • female but not male mice over 150 days in age have significant increases in body weight compared to littermate controls   (MGI Ref ID J:128233)
    • the increase is not as great as found in mice with the same transgene on a mixed (C57BL/6 and DBA/1) background   (MGI Ref ID J:128233)
  • nervous system phenotype
  • abnormal brain wave pattern
    • mice exhibit a higher electroencephalogram power densities at the theta and beta band during DREM than in wild-type mice indicating a cataplexy-like state   (MGI Ref ID J:141058)

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Tg(HCRT-MJD)1Stak/?

        involves: C57BL/6 * DBA/1
  • behavior/neurological phenotype
  • abnormal circadian feeding behavior
    • during the dark phase, mice consume 30% less food than wild-type mice   (MGI Ref ID J:69620)
  • abnormal sleep pattern
    • mice exhibit direct transitions from wake to REM unlike wild-type mice   (MGI Ref ID J:69620)
    • mice exhibit a decrease in the intervals between successive REM sleep periods and shorter latency to REM sleep compared to wild-type mice   (MGI Ref ID J:69620)
    • during the light period, mice exhibit shorter REM sleep and wake episodes compared to wild-type mice   (MGI Ref ID J:69620)
    • fragmentation of sleep/wake states
      • mice show severe sleep/wake fragmentation   (MGI Ref ID J:211045)
    • narcolepsy
      • beginning at 6 weeks of age, mice exhibit narcoleptic episodes with abrupt cessation of purposeful motor activity in which a sustained posture change is held until motion is abruptly resumed   (MGI Ref ID J:69620)
      • narcoleptic episodes occur during grooming or excited ambulation, last a few seconds to 150 seconds, and are often followed by increased feeding and drinking behaviors   (MGI Ref ID J:69620)
  • decreased food intake
    • during the dark phase, mice consume 30% less food than wild-type mice   (MGI Ref ID J:69620)
  • hypoactivity
    • during the dark period   (MGI Ref ID J:69620)
  • homeostasis/metabolism phenotype
  • decreased energy expenditure   (MGI Ref ID J:69620)
  • increased circulating leptin level
    • 53% increase in the circulating leptin levels to bodyweight ratio in female but not male mice   (MGI Ref ID J:128233)
  • growth/size/body phenotype
  • increased susceptibility to age related obesity
    • female but not male mice over 100 days in age have significant increases in body weight compared to littermate controls   (MGI Ref ID J:128233)
    • at 10 to 12 weeks of age, mice exhibit increased body weight compared to wild-type mice   (MGI Ref ID J:69620)
  • nervous system phenotype
  • neuron degeneration   (MGI Ref ID J:211045)
    • by 2 and 12 weeks of age 56% and 99%, respectively, of orexin-containing neurons are lost   (MGI Ref ID J:69620)

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Tg(HCRT-MJD)1Stak
Allele Name transgene insertion 1, Takeshi Sakurai
Allele Type Transgenic (Inserted expressed sequence)
Common Name(s) ORX/ATX-Tg; orexin/ataxin-3;
Strain of Origin(C57BL/6 x DBA/1)F1
Expressed Gene ATXN3, ataxin 3, human
Promoter HCRT, hypocretin (orexin) neuropeptide precursor, human
General Note Phenotypic Similarity to Human Syndrome: Narcolepsy (J:69620, J:204366)
Molecular Note The transgenic construct contained a carboxy terminal MJD cDNA fragment derived from a patient with Machado-Joseph disease adjoined to a human HCRT promoter. The promoter region consisted of 3.2 kb of 5' upstream region in addition to the the noncoding exon 1. Expression was directed specifically in hypocretin neurons. The MJD fragment contained 77 polyglutamine repeats and was tagged with a Myc epitope tag for histological examination. Expression of the MJD fragment resulted in the ablation of hypocretin containing neurons [MGI Ref ID J:69620]
 
 

Genotyping

Genotyping Information

Genotyping Protocols

Tg(HCRT-MJD)1Stak, High Resolution Melting


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Hara J; Beuckmann CT; Nambu T; Willie JT; Chemelli RM; Sinton CM; Sugiyama F; Yagami K; Goto K; Yanagisawa M; Sakurai T. 2001. Genetic ablation of orexin neurons in mice results in narcolepsy, hypophagia, and obesity. Neuron 30(2):345-54. [PubMed: 11394998]  [MGI Ref ID J:69620]

Zhang W; Sakurai T; Fukuda Y; Kuwaki T. 2006. Orexin neuron-mediated skeletal muscle vasodilation and shift of baroreflex during defense response in mice. Am J Physiol Regul Integr Comp Physiol 290(6):R1654-63. [PubMed: 16410401]  [MGI Ref ID J:201120]

Additional References

Tg(HCRT-MJD)1Stak related

Akiyama M; Yuasa T; Hayasaka N; Horikawa K; Sakurai T; Shibata S. 2004. Reduced food anticipatory activity in genetically orexin (hypocretin) neuron-ablated mice. Eur J Neurosci 20(11):3054-62. [PubMed: 15579160]  [MGI Ref ID J:101276]

Black SW; Morairty SR; Chen TM; Leung AK; Wisor JP; Yamanaka A; Kilduff TS. 2014. GABAB agonism promotes sleep and reduces cataplexy in murine narcolepsy. J Neurosci 34(19):6485-94. [PubMed: 24806675]  [MGI Ref ID J:211046]

Fujiki N; Yoshida Y; Zhang S; Sakurai T; Yanagisawa M; Nishino S. 2006. Sex difference in body weight gain and leptin signaling in hypocretin/orexin deficient mouse models. Peptides 27(9):2326-31. [PubMed: 16626839]  [MGI Ref ID J:128233]

Furutani N; Hondo M; Kageyama H; Tsujino N; Mieda M; Yanagisawa M; Shioda S; Sakurai T. 2013. Neurotensin co-expressed in orexin-producing neurons in the lateral hypothalamus plays an important role in regulation of sleep/wakefulness States. PLoS One 8(4):e62391. [PubMed: 23620827]  [MGI Ref ID J:200101]

Hara J; Gerashchenko D; Wisor JP; Sakurai T; Xie X; Kilduff TS. 2009. Thyrotropin-releasing hormone increases behavioral arousal through modulation of hypocretin/orexin neurons. J Neurosci 29(12):3705-14. [PubMed: 19321767]  [MGI Ref ID J:158172]

Hara J; Yanagisawa M; Sakurai T. 2005. Difference in obesity phenotype between orexin-knockout mice and orexin neuron-deficient mice with same genetic background and environmental conditions. Neurosci Lett 380(3):239-42. [PubMed: 15862893]  [MGI Ref ID J:104865]

Hasegawa E; Yanagisawa M; Sakurai T; Mieda M. 2014. Orexin neurons suppress narcolepsy via 2 distinct efferent pathways. J Clin Invest 124(2):604-16. [PubMed: 24382351]  [MGI Ref ID J:208001]

Honda M; Eriksson KS; Zhang S; Tanaka S; Lin L; Salehi A; Hesla PE; Maehlen J; Gaus SE; Yanagisawa M; Sakurai T; Taheri S; Tsuchiya K; Honda Y; Mignot E. 2009. IGFBP3 colocalizes with and regulates hypocretin (orexin). PLoS ONE 4(1):e4254. [PubMed: 19158946]  [MGI Ref ID J:144841]

Kelz MB; Sun Y; Chen J; Cheng Meng Q; Moore JT; Veasey SC; Dixon S; Thornton M; Funato H; Yanagisawa M. 2008. An essential role for orexins in emergence from general anesthesia. Proc Natl Acad Sci U S A 105(4):1309-14. [PubMed: 18195361]  [MGI Ref ID J:143966]

Mieda M; Williams SC; Sinton CM; Richardson JA; Sakurai T; Yanagisawa M. 2004. Orexin neurons function in an efferent pathway of a food-entrainable circadian oscillator in eliciting food-anticipatory activity and wakefulness. J Neurosci 24(46):10493-501. [PubMed: 15548664]  [MGI Ref ID J:96585]

Mieda M; Willie JT; Hara J; Sinton CM; Sakurai T; Yanagisawa M. 2004. Orexin peptides prevent cataplexy and improve wakefulness in an orexin neuron-ablated model of narcolepsy in mice. Proc Natl Acad Sci U S A 101(13):4649-54. [PubMed: 15070772]  [MGI Ref ID J:89241]

Mori T; Ito S; Kuwaki T; Yanagisawa M; Sakurai T; Sawaguchi T. 2010. Monoaminergic neuronal changes in orexin deficient mice. Neuropharmacology 58(4-5):826-32. [PubMed: 19703479]  [MGI Ref ID J:179565]

Tabuchi S; Tsunematsu T; Black SW; Tominaga M; Maruyama M; Takagi K; Minokoshi Y; Sakurai T; Kilduff TS; Yamanaka A. 2014. Conditional ablation of orexin/hypocretin neurons: a new mouse model for the study of narcolepsy and orexin system function. J Neurosci 34(19):6495-509. [PubMed: 24806676]  [MGI Ref ID J:211045]

Toyama S; Sakurai T; Tatsumi K; Kuwaki T. 2009. Attenuated phrenic long-term facilitation in orexin neuron-ablated mice. Respir Physiol Neurobiol 168(3):295-302. [PubMed: 19664728]  [MGI Ref ID J:155632]

Tsujino N; Tsunematsu T; Uchigashima M; Konno K; Yamanaka A; Kobayashi K; Watanabe M; Koyama Y; Sakurai T. 2013. Chronic alterations in monoaminergic cells in the locus coeruleus in orexin neuron-ablated narcoleptic mice. PLoS One 8(7):e70012. [PubMed: 23922890]  [MGI Ref ID J:204366]

Xie X; Wisor JP; Hara J; Crowder TL; LeWinter R; Khroyan TV; Yamanaka A; Diano S; Horvath TL; Sakurai T; Toll L; Kilduff TS. 2008. Hypocretin/orexin and nociceptin/orphanin FQ coordinately regulate analgesia in a mouse model of stress-induced analgesia. J Clin Invest 118(7):2471-81. [PubMed: 18551194]  [MGI Ref ID J:137688]

Yang L; Zou B; Xiong X; Pascual C; Xie J; Malik A; Xie J; Sakurai T; Xie XS. 2013. Hypocretin/orexin neurons contribute to hippocampus-dependent social memory and synaptic plasticity in mice. J Neurosci 33(12):5275-84. [PubMed: 23516292]  [MGI Ref ID J:196592]

Zhang S; Zeitzer JM; Sakurai T; Nishino S; Mignot E. 2007. Sleep/wake fragmentation disrupts metabolism in a mouse model of narcolepsy. J Physiol 581(Pt 2):649-63. [PubMed: 17379635]  [MGI Ref ID J:141058]

Zhang W; Sunanaga J; Takahashi Y; Mori T; Sakurai T; Kanmura Y; Kuwaki T. 2010. Orexin neurons are indispensable for stress-induced thermogenesis in mice. J Physiol 588(Pt 21):4117-29. [PubMed: 20807795]  [MGI Ref ID J:179546]

Zhang W; Zhang N; Sakurai T; Kuwaki T. 2009. Orexin neurons in the hypothalamus mediate cardiorespiratory responses induced by disinhibition of the amygdala and bed nucleus of the stria terminalis. Brain Res 1262:25-37. [PubMed: 19368849]  [MGI Ref ID J:148093]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, hemizygous mice may be bred together, to wildtype siblings, or to C57BL/6J inbred mice (Stock No. 000664). The Donating Investigator has not attempted to make the strain homozygous.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2140.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2782.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.


See Terms of Use tab for General Terms and Conditions


The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
Ordering Information
JAX® Mice
Surgical and Preconditioning Services
JAX® Services
Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form

Terms of Use

Terms of Use


General Terms and Conditions


Contact information

General inquiries regarding Terms of Use

Contracts Administration

phone:207-288-6470

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


(6.8)