|This Kiss1-hrGFP transgenic line expresses humanized Renilla Green Fluorescent Protein in Kiss1 (kisspeptin) expressing cells and neurons and has applications in studies of the gonadotropic axis regulation and reproductive function.|
Type Coisogenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Mating System Noncarrier x Hemizygote (Female x Male) 06-DEC-13 Mating System Hemizygote x Noncarrier (Female x Male) 06-DEC-13 Species laboratory mouse Donating Investigator Carol Elias, University of Michigan
The Kiss1 (KiSS-1 metastasis-suppressor) gene encodes a metastasis suppressor protein that is active in melanomas and breast carcinomas, and also acts to promote secretion of gonadotropin-released hormone. These transgenic mice express hrGFP, humanized Renilla Green Fluorescent Protein, under the control of the mouse Kiss1 promoter. The expression pattern of hrGFP mimics the endogenous Kiss1 gene (mRNA) expression pattern. hrGFP expression is detected in Kiss1 expression neurons of the arcuate nucleus and anteroventral periventricular nucleus of the hypothalamus as well as in the placenta, pancreatic beta-cells and Leydig cells. The Donating Investigator reports homozygous mice are viable and fertile.
Drs. Carol F. Elias and Jeffrey M. Zigman (The University of Texas Southwestern Medical Center, Dallas) modified the RP24-299B2 BAC, which contains the entire Kiss1 gene, by inserting coding sequence for hrGFP, humanized Renilla reniformis Green Fluorescent Protein, into the Kiss1 translational start site. The RP24-299B2 BAC contains the entire Kiss1 gene as well as approximately 109.49 kb sequence upstream of the Kiss1 start codon and approximately 69.01kb sequence downstream of the Kiss1 stop codon. DNA from a correctly modified BAC clone was used as the source for the transgene that was microinjected into the pronucleus of fertilized oocytes from C57BL/6 mice. Founder line KG26 was subsequently established. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.
|Considerations for Choosing Controls|
Strains carrying other alleles of Kiss1View Strains carrying other alleles of Kiss1 (2 strains)
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|Allele Name||transgene insertion KG26, Carol F Elias|
|Allele Type||Transgenic (Reporter)|
|Strain of Origin||C57BL/6|
|Expressed Gene||hrGFP, humanized Renilla Green Fluorescent Protein,|
|Promoter||Kiss1, KiSS-1 metastasis-suppressor, mouse, laboratory|
|Molecular Note||The transgene was generated from a modified RP24-299B2 BAC, which contains the entire Kiss1 gene, byinserting coding sequence for hrGFP, humanized Renilla reniformis Green Fluorescent Protein, into the Kiss1 translational start site. The RP24-299B2 BACcontains the entire Kiss1 gene as well as approximately 109.49 kb sequence upstream of the Kiss1 start codon and approximately 69.01kb sequence downstream of the Kiss1 stop codon. Line KG26 was generated. [MGI Ref ID J:200193]|
Breeding & Husbandry When maintaining a live colony, hemizygous mice may be bred together, to wildtype siblings, or to C57BL/6J inbred mice (Stock No. 000664). The Donating Investigator reports that homozygotes are viable and fertile. Mating System Noncarrier x Hemizygote (Female x Male) 06-DEC-13 Hemizygote x Noncarrier (Female x Male) 06-DEC-13
This strain is currently Under Development for Cryo.
|Considerations for Choosing Controls|
|Control Pricing Information for Genetically Engineered Mutant Strains.|
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