Strain Name:


Stock Number:



Under Development for Cryo

Register Interest
This Kiss1-hrGFP transgenic line expresses humanized Renilla Green Fluorescent Protein in Kiss1 (kisspeptin) expressing cells and neurons and has applications in studies of the gonadotropic axis regulation and reproductive function.


The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Coisogenic; Mutant Strain; Transgenic;
Additional information on Genetically Engineered and Mutant Mice.
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Mating SystemNoncarrier x Hemizygote         (Female x Male)   06-DEC-13
Mating SystemHemizygote x Noncarrier         (Female x Male)   06-DEC-13
Specieslaboratory mouse
Donating Investigator Carol Elias,   University of Michigan

The Kiss1 (KiSS-1 metastasis-suppressor) gene encodes a metastasis suppressor protein that is active in melanomas and breast carcinomas, and also acts to promote secretion of gonadotropin-released hormone. These transgenic mice express hrGFP, humanized Renilla Green Fluorescent Protein, under the control of the mouse Kiss1 promoter. The expression pattern of hrGFP mimics the endogenous Kiss1 gene (mRNA) expression pattern. hrGFP expression is detected in Kiss1 expression neurons of the arcuate nucleus and anteroventral periventricular nucleus of the hypothalamus as well as in the placenta, pancreatic beta-cells and Leydig cells. The Donating Investigator reports homozygous mice are viable and fertile.

Drs. Carol F. Elias and Jeffrey M. Zigman (The University of Texas Southwestern Medical Center, Dallas) modified the RP24-299B2 BAC, which contains the entire Kiss1 gene, by inserting coding sequence for hrGFP, humanized Renilla reniformis Green Fluorescent Protein, into the Kiss1 translational start site. The RP24-299B2 BAC contains the entire Kiss1 gene as well as approximately 109.49 kb sequence upstream of the Kiss1 start codon and approximately 69.01kb sequence downstream of the Kiss1 stop codon. DNA from a correctly modified BAC clone was used as the source for the transgene that was microinjected into the pronucleus of fertilized oocytes from C57BL/6 mice. Founder line KG26 was subsequently established. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.

Control Information

   000664 C57BL/6J
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Kiss1
023426   C57BL/6-Tg(Kiss1-cre)J2-4Cfe/J
017701   STOCK Kiss1tm1.1(cre/EGFP)Stei/J
View Strains carrying other alleles of Kiss1     (2 strains)


Phenotype Information

View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Neurobiology Research
Fluorescent protein expression in neural tissue

Research Tools
Fluorescent Proteins

Genes & Alleles

Gene & Allele Information provided by MGI

Allele Symbol Tg(Kiss1-hrGFP)KG26Cfe
Allele Name transgene insertion KG26, Carol F Elias
Allele Type Transgenic (Reporter)
Strain of OriginC57BL/6
Expressed Gene hrGFP, humanized Renilla Green Fluorescent Protein,
Promoter Kiss1, KiSS-1 metastasis-suppressor, mouse, laboratory
Molecular Note The transgene was generated from a modified RP24-299B2 BAC, which contains the entire Kiss1 gene, byinserting coding sequence for hrGFP, humanized Renilla reniformis Green Fluorescent Protein, into the Kiss1 translational start site. The RP24-299B2 BACcontains the entire Kiss1 gene as well as approximately 109.49 kb sequence upstream of the Kiss1 start codon and approximately 69.01kb sequence downstream of the Kiss1 stop codon. Line KG26 was generated. [MGI Ref ID J:200193]


Genotyping Information

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Helpful Links

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References provided by MGI

Selected Reference(s)

Cravo RM; Frazao R; Perello M; Osborne-Lawrence S; Williams KW; Zigman JM; Vianna C; Elias CF. 2013. Leptin signaling in Kiss1 neurons arises after pubertal development. PLoS One 8(3):e58698. [PubMed: 23505551]  [MGI Ref ID J:200193]

Health & husbandry

Health & Colony Maintenance Information

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, hemizygous mice may be bred together, to wildtype siblings, or to C57BL/6J inbred mice (Stock No. 000664). The Donating Investigator reports that homozygotes are viable and fertile.
Mating SystemNoncarrier x Hemizygote         (Female x Male)   06-DEC-13
Hemizygote x Noncarrier         (Female x Male)   06-DEC-13

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


This strain is currently Under Development for Cryo.

Control Information

   000664 C57BL/6J
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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