Strain Name:

STOCK Vapbtm1.2Ics/J

Stock Number:


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Under Development - Now Accepting Orders

Estimated Available for Distribution Date: 28-JUL-14
Use Restrictions Apply, see Terms of Use
These Vspb-/- mice may be useful for studying risk factors for motor neuron diseases such as amyotrophic lateral sclerosis (ALS).


Strain Information

Former Names B6N.129S2(Cg)-Vapbtm1.2Ics/J    (Changed: 28-MAR-14 )
Type Mutant Stock; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
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Mating SystemHeterozygote x Heterozygote         (Female x Male)   06-MAY-14
Specieslaboratory mouse
GenerationN6pN1 (10-JUN-14)
Generation Definitions
Donating Investigator Luc Dupuis,   Universite de Strasbourg

These Vspb-/- mutant mice lack exon 3 of the vesicle-associated membrane protein, associated protein B and C (Vapb) gene, abolishing gene expression. Vspb encodes a membrane protein involved in vesicle trafficking, membrane fusion, protein complex assembly, and cell motility. Mutations and loss of VAPB function have been shown to contribute to motor deficits associated with amyotrophic lateral sclerosis (ALS) pathogenesis. Homozygotes are viable and fertile. Adult Vapb KO mice develop mild, late onset central nervous system defects including mild motor deficits after 18 months of age and mild tremors in open field tests. These mice do not exhibit defects in neuromuscular junction or muscle strength, and there is no apparent muscle denervation.

A targeting vector was designed to insert a loxP site upstream of exon 3, and a second loxP site followed by a frt-flanked PGK-neo cassette just downstream of exon 3 of the vesicle-associated membrane protein, associated protein B and C (Vapb) gene. The construct was electroporated into 129S2/SvPas-derived P1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and resulting chimeric males were bred to mice expressing Frt-recombinase to remove the neo cassette. Offspring were crossed to remove the Frt-containing transgene and progeny were bred to CMV-cre transgenic mice to remove the floxed-sequence. Subsequently, these Vspb-/- mice were bred to C57BL/6NTac mice once and were maintained on a mixed background. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6NJ (Stock No. 005304) for at least one generation to establish the colony.

Control Information

   None Available (approximate)
  Considerations for Choosing Controls


Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested.
Amyotrophic Lateral Sclerosis 8; ALS8   (VAPB)
Spinal Muscular Atrophy, Late-Onset, Finkel Type; SMAFK   (VAPB)
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.


  • behavior/neurological phenotype
  • impaired coordination
    • at 18, but not 4, months   (MGI Ref ID J:198145)
  • tremors
    • mild in an open field   (MGI Ref ID J:198145)
  • muscle phenotype
  • hypotonia
    • in the hind limb and abdomen of older mice   (MGI Ref ID J:198145)
    • however, muscle strength in a grip test is normal   (MGI Ref ID J:198145)
  • nervous system phenotype
  • *normal* nervous system phenotype
    • mice exhibit normal neuromuscular junctions with no denervation   (MGI Ref ID J:198145)
  • cellular phenotype
  • *normal* cellular phenotype
    • mice exhibit normal basal and tunicamycin-induce unfolded protein response   (MGI Ref ID J:198145)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Cell Biology Research
Vesicular Trafficking

Neurobiology Research
Amyotrophic Lateral Sclerosis (ALS)
Ataxia (Movement) Defects
Cre-lox System
      loxP-flanked Sequences

Research Tools
Cre-lox System
      loxP-flanked Sequences

Genes & Alleles

Gene & Allele Information provided by MGI

Allele Symbol Vapbtm1.2Ics
Allele Name targeted mutation 1.2, Mouse Clinical Institute
Allele Type Targeted (Null/Knockout)
Common Name(s) Vapbdeltaexon3; Vapbtm1.2Ldps;
Strain of Origin129S2/SvPas
Gene Symbol and Name Vapb, vesicle-associated membrane protein, associated protein B and C
Chromosome 2
Gene Common Name(s) AI225786; AI840687; AI848259; ALS8; D2Abb2e; DNA segment, Chr 2, Abbott 2 expressed; R75548; VAMP-B; VAMP-associated protein 33b; VAP-B; VAP33b; expressed sequence AI225786; expressed sequence AI840687; expressed sequence AI848259; expressed sequence R75548;
Molecular Note Cre-mediated recombination removed a floxed neomycin resistance cassette. Flp-mediated recombination removed the FRT-flanked neomycin resistance cassette. Western blot anlaysis confirmed the absence of protein expression in the cerebral cortex. [MGI Ref ID J:198145]


Genotyping Information

Genotyping Protocols

Vapbtm1.2Ics, Standard PCR

Helpful Links

Genotyping resources and troubleshooting


References provided by MGI

Selected Reference(s)

Bello EP; Mateo Y; Gelman DM; Noain D; Shin JH; Low MJ; Alvarez VA; Lovinger DM; Rubinstein M. 2011. Cocaine supersensitivity and enhanced motivation for reward in mice lacking dopamine D2 autoreceptors. Nat Neurosci 14(8):1033-8. [PubMed: 21743470]  [MGI Ref ID J:176167]

Additional References

Vapbtm1.2Ics related

Kabashi E; El Oussini H; Bercier V; Gros-Louis F; Valdmanis PN; McDearmid J; Mejier IA; Dion PA; Dupre N; Hollinger D; Sinniger J; Dirrig-Grosch S; Camu W; Meininger V; Loeffler JP; Rene F; Drapeau P; Rouleau GA; Dupuis L. 2013. Investigating the contribution of VAPB/ALS8 loss of function in amyotrophic lateral sclerosis. Hum Mol Genet 22(12):2350-60. [PubMed: 23446633]  [MGI Ref ID J:198145]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX10

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, homozygous mice may be bred together.
Mating SystemHeterozygote x Heterozygote         (Female x Male)   06-MAY-14

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


This strain is currently Under Development - Now Accepting Orders.
Estimated Available for Distribution Date: 28-JUL-14

Please note: You may now place orders for this strain although it is not yet ready for distribution. Estimated available for distribution dates are provided to keep customers better informed on strains under development. Please note that our Colony Managers routinely monitor the target date and edit it based on breeding performance and other factors. The length of time it takes to make a new strain available for distribution depends on genotype, age, number of animals sent by the Donating Investigator, breeding performance, additional strain development (backcrossing, making homozygous), and anticipated demand for the strain.

Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $232.00Female or MaleHeterozygous for Vapbtm1.2Ics  
$232.00Female or MaleHomozygous for Vapbtm1.2Ics  
Price per Pair (US dollars $)Pair Genotype
$464.00Heterozygous for Vapbtm1.2Ics x Heterozygous for Vapbtm1.2Ics  

Standard Supply

Under Development - Now Accepting Orders The strain development process (i.e. importation, rederivation, and colony expansion) usually takes six to nine months.

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $301.60Female or MaleHeterozygous for Vapbtm1.2Ics  
$301.60Female or MaleHomozygous for Vapbtm1.2Ics  
Price per Pair (US dollars $)Pair Genotype
$603.20Heterozygous for Vapbtm1.2Ics x Heterozygous for Vapbtm1.2Ics  

Standard Supply

Under Development - Now Accepting Orders The strain development process (i.e. importation, rederivation, and colony expansion) usually takes six to nine months.

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Under Development - Now Accepting Orders The strain development process (i.e. importation, rederivation, and colony expansion) usually takes six to nine months.

Control Information

   None Available (approximate)
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

See Terms of Use tab for General Terms and Conditions

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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Terms of Use

Terms of Use

General Terms and Conditions

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JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

No Warranty


In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

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The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

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