Strain Name:

STOCK Dact1tm1.2Bnrc/J

Stock Number:

023715

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Availability:

Under Development - Now Accepting Orders

Estimated Available for Distribution Date: 17-NOV-14
Use Restrictions Apply, see Terms of Use
These Dact1flox mice may be useful for studying embryonic and neuronal development.

Description

Strain Information

Former Names B6.129S(Cg)-Dact1tm1.2Bnrc/J    (Changed: 16-APR-14 )
Type Mutant Stock; Targeted Mutation;
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Mating SystemHeterozygote x Heterozygote         (Female x Male)   28-MAY-14
Specieslaboratory mouse
 
Donating Investigator Benjamin N R Cheyette,   UCSF School of Medicine

Description
These Dact1flox mice possess loxP sites flanking exon 2 of the dapper homolog 1, antagonist of beta-catenin (Dact1) gene. Dact1 encodes an intracellual protein required for morphogenesis at the primitive streak, and for dendrite, spine, and excitatory synapse development in the mouse forebrain. Through interactions with the Dishevelled family of proteins, DACT1 modulates the transcriptional activation of target genes of the Wnt/beta-catenin signaling pathway as well as beta-catenin-independent branches of Wnt signaling. Homozygotes are viable and fertile. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exon 2 deleted in the cre-expressing tissues.

For example, when bred to B6.129S2-Emx1tm1(cre)Krj/J mice (Stock No. 005628) expressing Cre recombinase in the neurons of the neocortex and hippocampus, neurons from double mutant Dact1-deficient mice exhibit reduced dendritic spine density, spine length, and spine head width.

Development
A targeting vector was designed to insert a frt-flanked neomycin resistance (neo) cassette, followed by a loxP site, upstream of exon 2, and a second loxP site downstream of exon 2 of the dapper homolog 1, antagonist of beta-catenin (Dact1) gene. The construct was electroporated into 129Sv embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and resulting chimeric mice were bred with B6.Cg-Tg(ACTFLPe)9205Dym/J transgenic mice (Stock No. 005703) to delete the neo cassette. Progeny were crossed to remove the Flp-expressing transgene. These mice were bred to C57BL/6 mice for at least 10 generations. Subsequenty, they have been bred to CAG-CAT-EGFP transgenic mice on a CD-1 background. Upon arrival, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony. The CAG-CAT-EGFP transgene was removed from the colony.

Control Information

  Control
   None Available (approximate)
 
  Considerations for Choosing Controls

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype relates to a compound genotype created using this strain.
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Dact1tm1.1Bnrc/Dact1tm1.2Bnrc Emx1tm1(cre)Krj/Emx1+

        involves: 129S2/SvPas   (conditional)
  • nervous system phenotype
  • *normal* nervous system phenotype
    • mice exhibit normal cellular organization and regional anatomy of the brain   (MGI Ref ID J:159217)
    • abnormal dendritic spine morphology
      • cultured hippocampal neurons exhibit reduced dendritic spine density, spine length, and spine head width compared with wild-type neurons   (MGI Ref ID J:159217)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Developmental Biology Research
Neurodevelopmental Defects

Neurobiology Research
Cre-lox System
      loxP-flanked Sequences

Research Tools
Cre-lox System
      loxP-flanked Sequences

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Dact1tm1.2Bnrc
Allele Name targeted mutation 1.2, Benjamin N R Cheyette
Allele Type Targeted (Conditional ready (e.g. floxed), No functional change)
Common Name(s) Dact1flox;
Strain of Origin129
Gene Symbol and Name Dact1, dapper homolog 1, antagonist of beta-catenin (xenopus)
Chromosome 12
Gene Common Name(s) 4921528D17Rik; AI115603; DAPPER; DAPPER1; DPR1; FRODO; Frd1; Frodo1; HDPR1; RGD1564008; RIKEN cDNA 4921528D17 gene; THYEX3; expressed sequence AI115603;
Molecular Note A targeting vector was designed to insert a frt-flanked neomycin resistance (neo) cassette, followed by a loxP site, upstream of exon 2, and a second loxP site downstream of exon 2. Flp-mediated recombination removed the neo cassette leaving exon 2 floxed. [MGI Ref ID J:152701] [MGI Ref ID J:159217]

Genotyping

Genotyping Information

Genotyping Protocols

Dact1tm1.2Bnrc, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Okerlund ND; Kivimae S; Tong CK; Peng IF; Ullian EM; Cheyette BN. 2010. Dact1 is a postsynaptic protein required for dendrite, spine, and excitatory synapse development in the mouse forebrain. J Neurosci 30(12):4362-8. [PubMed: 20335472]  [MGI Ref ID J:159217]

Suriben R; Kivimae S; Fisher DA; Moon RT; Cheyette BN. 2009. Posterior malformations in Dact1 mutant mice arise through misregulated Vangl2 at the primitive streak. Nat Genet 41(9):977-85. [PubMed: 19701191]  [MGI Ref ID J:152701]

Additional References

Dact1tm1.2Bnrc related

Arguello A; Yang X; Vogt D; Stanco A; Rubenstein JL; Cheyette BN. 2013. Dapper antagonist of catenin-1 cooperates with Dishevelled-1 during postsynaptic development in mouse forebrain GABAergic interneurons. PLoS One 8(6):e67679. [PubMed: 23826333]  [MGI Ref ID J:203731]

Health & husbandry

Health & Colony Maintenance Information

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, homozygous mice may be bred together.
Mating SystemHeterozygote x Heterozygote         (Female x Male)   28-MAY-14

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


 

This strain is currently Under Development - Now Accepting Orders.
Estimated Available for Distribution Date: 17-NOV-14

Please note: You may now place orders for this strain although it is not yet ready for distribution. Estimated available for distribution dates are provided to keep customers better informed on strains under development. Please note that our Colony Managers routinely monitor the target date and edit it based on breeding performance and other factors. The length of time it takes to make a new strain available for distribution depends on genotype, age, number of animals sent by the Donating Investigator, breeding performance, additional strain development (backcrossing, making homozygous), and anticipated demand for the strain.

Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $232.00Female or MaleHeterozygous for Dact1tm1.2Bnrc  
$232.00Female or MaleHomozygous for Dact1tm1.2Bnrc  
Price per Pair (US dollars $)Pair Genotype
$464.00Heterozygous for Dact1tm1.2Bnrc x Heterozygous for Dact1tm1.2Bnrc  

Standard Supply

Under Development - Now Accepting Orders The strain development process (i.e. importation, rederivation, and colony expansion) usually takes six to nine months.

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $301.60Female or MaleHeterozygous for Dact1tm1.2Bnrc  
$301.60Female or MaleHomozygous for Dact1tm1.2Bnrc  
Price per Pair (US dollars $)Pair Genotype
$603.20Heterozygous for Dact1tm1.2Bnrc x Heterozygous for Dact1tm1.2Bnrc  

Standard Supply

Under Development - Now Accepting Orders The strain development process (i.e. importation, rederivation, and colony expansion) usually takes six to nine months.

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Under Development - Now Accepting Orders The strain development process (i.e. importation, rederivation, and colony expansion) usually takes six to nine months.

Control Information

  Control
   None Available (approximate)
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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