Strain Name:

B6.Cg-Tg(Omp-Hist2h2be)14Dlc/J

Stock Number:

023820

Order this mouse

Availability:

Repository- Live

Use Restrictions Apply, see Terms of Use
These transgenic mice carry flag-tagged mouse Hist2h2be (histone cluster 2, H2be) downstream of the rat Omp (olfactory marker protein) promoter. High levels of FLAG-H2BE are seen in all mature olfactory neurons except for a subset in zone 2.

Description

Strain Information

Type Mutant Strain; Transgenic;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Mating SystemNoncarrier x Hemizygote         (Female x Male)   20-JAN-14
Mating SystemHemizygote x Noncarrier         (Female x Male)   20-JAN-14
Specieslaboratory mouse
GenerationN5+pN1 (04-FEB-14)
Generation Definitions
 
Donating Investigator Catherine Dulac,   Harvard University, HHMI

Description
Hist2h2be (histone cluster 2, H2be) is expressed exclusively in olfactory chemosensory neurons. Expression is reduced by sensory activity, and it alters gene expression and promotes neuronal cell death in mature olfactory sensory neurons, potentially modulating olfactory neuronal population dynamics to adapt the olfactory repertoire to the environment.

These transgenic mice carry flag-tagged mouse Hist2h2be (histone cluster 2, H2be) downstream of the rat Omp (olfactory marker protein) promoter. The flag reporter facilitates and characterization and unambiguous identification of Hist2h2be and distinguishes it from canonical H2B. Immunofluorescent examination in the main olfactory epithelium (MOE), reveals a 10 fold increase in nuclear expression in a pattern similar to that of endogenous OMP, with the exception of a small patch of neurons in the medial part of the MOE that lack expression. High levels of FLAG-H2BE are seen in all mature olfactory neurons except for a subset in zone 2. Transgenic mice display higher levels of apoptosis among olfactory sensory neurons in the MOE.

Development
A transgenic vector carrying flag-tagged mouse Hist2h2be was placed downstream of the rat Omp (olfactory marker protein) promoter was introduced to C57BL/6J x DBA/2J (B6D2F1) embryos. Resultant founder line 14, carrying an estimated 12 copies of the transgene, was backcrossed to C57BL/6J for approximately 5 generations by the donating laboratory.

Control Information

  Control
   Noncarrier
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Hist2h2be
023821   B6.Cg-Tg(Hist2h2be)2Dlc/J
023819   B6;129S4-Hist2h2betm1Dlc/J
View Strains carrying other alleles of Hist2h2be     (2 strains)

View Strains carrying other alleles of Omp     (7 strains)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Tg(Omp-Hist2h2be)14Dlc/0

        involves: C57BL/6
  • nervous system phenotype
  • abnormal olfactory sensory neuron morphology   (MGI Ref ID J:197878)
  • increased neuron apoptosis   (MGI Ref ID J:197878)
  • cellular phenotype
  • increased neuron apoptosis   (MGI Ref ID J:197878)
  • craniofacial phenotype
  • abnormal olfactory sensory neuron morphology   (MGI Ref ID J:197878)
  • respiratory system phenotype
  • abnormal olfactory sensory neuron morphology   (MGI Ref ID J:197878)
  • taste/olfaction phenotype
  • abnormal olfactory sensory neuron morphology   (MGI Ref ID J:197878)
  • growth/size/body phenotype
  • abnormal olfactory sensory neuron morphology   (MGI Ref ID J:197878)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Sensorineural Research
Olfactory Defects

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Tg(Omp-Hist2h2be)14Dlc
Allele Name transgene insertion 14, Catherine Dulac
Allele Type Transgenic (Inserted expressed sequence)
Common Name(s) H2be-GF; Omp:Flag-H2be; Tg(Omp-Hist2h2be)#aDlc;
Strain of Origin(C57BL/6J x DBA/2J)F1
Expressed Gene Hist2h2be, histone cluster 2, H2be, mouse, laboratory
Promoter Omp, olfactory marker protein, mouse, laboratory
Molecular Note The promoter drives olfactory-specific expression of a FLAG-tagged coding sequence. Line 14 contains 12 copies of the transgene. [MGI Ref ID J:197878]
 
 

Genotyping

Genotyping Information

Genotyping Protocols

Tg(Omp-Hist2h2be)14Dlc, High Resolution Melting


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Additional References

Tg(Omp-Hist2h2be)14Dlc related

Santoro SW; Dulac C. 2012. The activity-dependent histone variant H2BE modulates the life span of olfactory neurons. Elife 1:e00070. [PubMed: 23240083]  [MGI Ref ID J:197878]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX10

Colony Maintenance

Breeding & HusbandryHeterozygous and homozygous mice are viable and fertile.
Mating SystemNoncarrier x Hemizygote         (Female x Male)   20-JAN-14
Hemizygote x Noncarrier         (Female x Male)   20-JAN-14
Diet Information LabDiet® 5K52/5K67

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $232.00Female or MaleHemizygous for Tg(Omp-Hist2h2be)14Dlc  
Price per Pair (US dollars $)Pair Genotype
$304.00Hemizygous for Tg(Omp-Hist2h2be)14Dlc x Noncarrier  
$304.00Noncarrier x Hemizygous for Tg(Omp-Hist2h2be)14Dlc  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $301.60Female or MaleHemizygous for Tg(Omp-Hist2h2be)14Dlc  
Price per Pair (US dollars $)Pair Genotype
$395.20Hemizygous for Tg(Omp-Hist2h2be)14Dlc x Noncarrier  
$395.20Noncarrier x Hemizygous for Tg(Omp-Hist2h2be)14Dlc  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Control Information

  Control
   Noncarrier
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.


See Terms of Use tab for General Terms and Conditions


The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
Ordering Information
JAX® Mice
Surgical and Preconditioning Services
JAX® Services
Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form

Terms of Use

Terms of Use


General Terms and Conditions


For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.

Contact information

General inquiries regarding Terms of Use

Contracts Administration

phone:207-288-6470

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


(6.8)