Strain Name:

B6.Cg-Tg(Hist2h2be)2Dlc/J

Stock Number:

023821

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Availability:

Under Development - Now Accepting Orders

Estimated Available for Distribution Date: 02-MAR-15
Use Restrictions Apply, see Terms of Use
These BAC transgenic mice carry a single copy of Flag-tagged Hist2h2be (histone cluster 2, H2be), likely integrated on the X-chromosome. Flag-H2be mice have no apparent phenotype differences from wildtype littermates, but males do not efficiently transfer the transgene. Embryonic viability may be compromised among female embryos carrying the transgene contributed from the father.

Description

Strain Information

Type Congenic; Mutant Strain; Transgenic;
Additional information on Genetically Engineered and Mutant Mice.
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Mating SystemHemizygote x Noncarrier         (Female x Male)   25-NOV-14
Specieslaboratory mouse
GenerationN5+pN1 (25-NOV-14)
Generation Definitions
 
Donating Investigator Catherine Dulac,   Harvard University, HHMI

Description
Hist2h2be (histone cluster 2, H2be) is expressed exclusively in olfactory chemosensory neurons. Expression is reduced by sensory activity, and it promotes neuronal cell death, potentially modulating olfactory neuron population dynamics to adapt the olfactory repertoire to the environment.

These BAC transgenic mice carry a single copy of Flag-tagged Hist2h2be, likely integrated on the X-chromosome. Expression of the Flag-H2be transgene was examined by RT-qPCR in the main olfactory epithelium (MOE), where the expression level of the transgenic allele was found to be identical to that of the endogenous Hist2h2be allele. Expression was also examined by immunofluorescence and in situ hybridization in the MOE, vomeronasal organ, and brain, revealing indistinguishable patterns of expression (or lack of expression, in the case of the brain) for FLAG-H2BE protein and endogenous Hist2h2be mRNA. Males do not efficiently transfer the transgene (see maintenance note for more details).

Development
A Flag-encoding DNA sequence was inserted upstream of the Hist2h2be coding region within BAC RP23-16G3, which contains a 200-kb region of mouse genomic sequence surrounding the gene. The modified BAC was amplified in E. coli, confirmed by sequencing, and injected into B6D2F1 (C57BL/6J x DBA/2J) embryos. Transgenic founders were crossed to C57BL/6J mice for approximately 5 generations to establish founder line 2 which carries 1 copy of the transgene.

Control Information

  Control
   Noncarrier
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Hist2h2be
023820   B6.Cg-Tg(Omp-Hist2h2be)14Dlc/J
023819   B6;129S4-Hist2h2betm1Dlc/J
View Strains carrying other alleles of Hist2h2be     (2 strains)

Phenotype

Phenotype Information

View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Developmental Biology Research
Embryonic Lethality (Homozygous)

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Tg(Hist2h2be)2Dlc
Allele Name transgene insertion 2, Catherine Dulac
Allele Type Transgenic (Inserted expressed sequence)
Common Name(s) Flag-H2be; H2be:Flag-H2be; Tg(Hist2h2be)#aDlc;
Strain of Origin(C57BL/6J x DBA/2)F1
Expressed Gene Hist2h2be, histone cluster 2, H2be, mouse, laboratory
Molecular Note A Flag-encoding DNA sequence was inserted upstream of the coding region within BAC RP23-16G3, which contains a 200-kb region of mouse genomic sequence surrounding the gene. Line 2 contains one copy of the transgene. [MGI Ref ID J:197878]
 

Genotyping

Genotyping Information

Genotyping Protocols

Tg(Hist2h2be)2Dlc,

MELT


Tg(Hist2h2be)2Dlc, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Santoro SW; Dulac C. 2012. The activity-dependent histone variant H2BE modulates the life span of olfactory neurons. Elife 1:e00070. [PubMed: 23240083]  [MGI Ref ID J:197878]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX12

Colony Maintenance

Breeding & HusbandryHemizygotes are viable and fertile. The transgene is reportedly transferred from the father very inefficiently. The transgene is likely located on the X-chromosome, as males produced from a cross between a hemizygous male and a wild type female are invariably wild type. In addition, very few females are produced from the same cross, indicating that there may be a viability problem among female embryos carrying the transgene from the father (although there is no problem transferring the transgene from the mother). These issues may make in vitro fertilization from cryopreserved transgenic sperm problematic.
Mating SystemHemizygote x Noncarrier         (Female x Male)   25-NOV-14

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


 

This strain is currently Under Development - Now Accepting Orders.
Estimated Available for Distribution Date: 02-MAR-15

Please note: You may now place orders for this strain although it is not yet ready for distribution. Estimated available for distribution dates are provided to keep customers better informed on strains under development. Please note that our Colony Managers routinely monitor the target date and edit it based on breeding performance and other factors. The length of time it takes to make a new strain available for distribution depends on genotype, age, number of animals sent by the Donating Investigator, breeding performance, additional strain development (backcrossing, making homozygous), and anticipated demand for the strain.

Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $232.00Female or MaleHemizygous for Tg(Hist2h2be)2Dlc  
Price per Pair (US dollars $)Pair Genotype
$304.00Hemizygous for Tg(Hist2h2be)2Dlc x Noncarrier  

Standard Supply

Under Development - Now Accepting Orders The strain development process (i.e. importation, rederivation, and colony expansion) usually takes six to nine months.

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $301.60Female or MaleHemizygous for Tg(Hist2h2be)2Dlc  
Price per Pair (US dollars $)Pair Genotype
$395.20Hemizygous for Tg(Hist2h2be)2Dlc x Noncarrier  

Standard Supply

Under Development - Now Accepting Orders The strain development process (i.e. importation, rederivation, and colony expansion) usually takes six to nine months.

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Under Development - Now Accepting Orders The strain development process (i.e. importation, rederivation, and colony expansion) usually takes six to nine months.

Control Information

  Control
   Noncarrier
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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