Strain Name:

B6.129S4-Prkcdbptm1Pemi/J

Stock Number:

023823

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Availability:

Repository- Live

Use Restrictions Apply, see Terms of Use
Cavin-3 knock-out mice exhibit cachexia and increased glycolytic metabolism. They are suitable for use in applications related to the study of ERK and Akt signaling.

Description

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
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Additional information on Congenic nomenclature.
Mating SystemHeterozygote x Heterozygote         (Female x Male)   05-FEB-14
Specieslaboratory mouse
GenerationN8+pN1 (21-APR-14)
Generation Definitions
 
Donating Investigator Peter Michaely,   UT Southwestern Medical Center

Description
Protein kinase C, delta binding protein, or Cavin-3, forms a complex with cavin-1 (polymerase I and transcript release factor) and caveolin to anchor caveolae to the plasma membrane at the actin cytoskeleton via myosin 1c protein. Cavin-3 is involved in ERK and AKT signaling and circadian clock regulation. These mice carry a targeted mutation in which exon 1 and most of exon 2 is replaced by a floxed NEO cassette. Homozygous mice are viable and fertile, although with a shortened lifespan. At 12 months of age homozygotes have an approximately 80% survival rate, dropping to an approximately 40% survival rate at 24 months of age. No gene product (protein) is detected by Western blot analysis of tissue (lung) or of MEFs isolated from homozygous animals. Homozygotes exhibit cachexia (40% reduction in body weight and severe lipodystrophy), hepatic steatosis, and increased glycolytic metabolism. MEFs from homozygous animals proliferate faster than MEFs from wildtype controls, exhibit Warburg metabolism, are more resistant to TNF-alpha and the MAPK/ERK Kinase (MEK) inhibitor, PD98059, and are more sensitive to the PI3K inhibitor, LY294002. During backcrossing, the Y chromosome may not have been fixed to the C57BL/6J genetic background.

Development
A targeting vector containing a loxP site flanked NEO cassette was used to disrupt exon 1 and most of exon 2. The construct was electroporated into 129S4/SvJae derived J1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric animals were tested for germline transmission. The mice were backcrossed to C57BL/6J for 8 generations. During backcrossing, the Y chromosome may not have been fixed to the C57BL/6J genetic background. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Prkcdbptm1Pemi/Prkcdbptm1Pemi

        B6.129S4-Prkcdbptm1Pemi
  • cellular phenotype
  • abnormal cellular respiration
    • increased fermentative glycolysis observed in lung tissue (Warburg metabolism)   (MGI Ref ID J:202374)
    • lung tissue from 6 week old mice has increased glucose consumption and increased lactate production   (MGI Ref ID J:202374)
  • adipose tissue phenotype
  • lipodystrophy
    • severe lipdystrophy as compared to wild type   (MGI Ref ID J:202374)
  • growth/size/body phenotype
  • decreased body weight
    • 40% reduction in body mass by 24 months as compared to wild type   (MGI Ref ID J:202374)
  • lipodystrophy
    • severe lipdystrophy as compared to wild type   (MGI Ref ID J:202374)
  • liver/biliary system phenotype
  • hepatic steatosis
    • areas of steatosis observed in liver   (MGI Ref ID J:202374)
  • mortality/aging
  • premature death
    • 40% of mice die by 24 months of age   (MGI Ref ID J:202374)
    • death is the result of cachexia   (MGI Ref ID J:202374)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Cell Biology Research
Genes Regulating Growth and Proliferation
Signal Transduction

Internal/Organ Research
Liver Defects

Metabolism Research
Lipid Metabolism

Research Tools
Metabolism Research

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Prkcdbptm1Pemi
Allele Name targeted mutation 1, Peter Michaely
Allele Type Targeted (Null/Knockout)
Common Name(s) Cavin-3 KO; Prkcdbp-;
Strain of Origin129S4/SvJae
Gene Symbol and Name Prkcdbp, protein kinase C, delta binding protein
Chromosome 7
Gene Common Name(s) 3110015B12Rik; 6330514M23Rik; CAVIN3; DIG-2; HSRBC; RIKEN cDNA 3110015B12 gene; RIKEN cDNA 6330514M23 gene; SRBC; cavin 3; cavin-3;
Molecular Note A targeting vector containing a loxP-flanked NEO cassette was used to disrupt exon 1 and most of exon 2. No gene product (protein) is detected by Western blot analysis of MEFs isolated from homozygous animals. [MGI Ref ID J:202374]

Genotyping

Genotyping Information

Genotyping Protocols

Prkcdbptm1Pemi-Alternate 1, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Additional References

Prkcdbptm1Pemi related

Hernandez VJ; Weng J; Ly P; Pompey S; Dong H; Mishra L; Schwarz M; Anderson RG; Michaely P. 2013. Cavin-3 dictates the balance between ERK and Akt signaling. Elife 2:e00905. [PubMed: 24069528]  [MGI Ref ID J:202374]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX18

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, these mice can be bred as homozygotes. Homozygotes have a shortened lifespan when compared to wildtype controls (approximately 40% survival rate at 24 months of age).
Mating SystemHeterozygote x Heterozygote         (Female x Male)   05-FEB-14
Diet Information LabDiet® 5K52/5K67

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $232.00Female or MaleHeterozygous for Prkcdbptm1Pemi  
$232.00Female or MaleHomozygous for Prkcdbptm1Pemi  
Price per Pair (US dollars $)Pair Genotype
$464.00Heterozygous for Prkcdbptm1Pemi x Heterozygous for Prkcdbptm1Pemi  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $301.60Female or MaleHeterozygous for Prkcdbptm1Pemi  
$301.60Female or MaleHomozygous for Prkcdbptm1Pemi  
Price per Pair (US dollars $)Pair Genotype
$603.20Heterozygous for Prkcdbptm1Pemi x Heterozygous for Prkcdbptm1Pemi  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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