Strain Name:

B6.Cg-Col1a1tm1(tetO-Lin28a)Gqda/J

Stock Number:

023910

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Availability:

Under Development - Now Accepting Orders

Estimated Available for Distribution Date: 27-OCT-14
Use Restrictions Apply, see Terms of Use
These knock-in mice express Lin28a under the regulatory control of a Col1a1-driven tetO promoter. These mice are larger than non-transgenic animals, even in the absence of tTA/rtTA, show delayed estrus, and a resistance to age/diet-induced obesity.

Description

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
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Additional information on Congenic nomenclature.
Mating SystemHeterozygote x Heterozygote         (Female x Male)   09-MAY-14
Specieslaboratory mouse
GenerationN8+pN1 (28-JUL-14)
Generation Definitions
 
Donating Investigator George Q. Daley,   Children's Hospital Boston

Description
LIN28A (lin-28 homolog A (C. elegans)) is an RNA-binding protein that blocks biogenesis of Mirlet7 (let-7) microRNAs. Let-7 targets are known regulators of mammalian body size and metabolism.

These knock-in mice express Lin28a under the regulatory control of a Col1a1 (collagen, type I, alpha 1)-driven tetO promoter. These mice are larger than non-transgenic animals, show wider faces, larger ears, and coarser hair, even in the absence of tTA/rtTA/doxycyline induction. RT-PCR demonstrates that there is some ectopic expression of the transgene in both neonates and adults. In adults, there is a sevenfold increase in the level of Lin28a mRNA in skeletal muscle, a fivefold increase in the ovary, and fourfold increase in the hypothalamus.

Fasting and fed glucose levels are lower in transgenic mice. Both males and females clear glucose more efficiently. Transgenic mice also have increased insulin sensitivity and are resistant to age-induced and diet-induced obesity, as well as hepatosteatosis. Mice show markedly improved glucose tolerance and insulin sensitivity under high fat diet conditions. Transgenic mice achieve first estrus at day 31.8, as compared to day 27.3 in wildtype mice. Transgenic animals show an approximated 3 day delay to the date of the first litter. Overall fertility in mutant and wildtype mice is about the same over the first 3 months of life.

Lin28a expression can be induced with doxycycline in progeny derived from a cross with a Gt(ROSA)26Sor M2-rtTA strain (see Stock No. 006965). This leads to rapid death associated with marked gut pathology. Uninduced mice have thickened skin, larger bones and proportionately enlarged visceral organs.

Uninduced Gt(ROSA)26Sor M2-rtTA/tetO-Lin28a compound mutant mice are said to have the same phenotype as animals carrying only the tetO-Lin28a mutation.

Development
A targeting vector containing a splice acceptor, 2 polyadenylation sequences, a tetracycline operator, flag-tagged mouse Lin28a cDNA, and an SV40 polyadenylation signal within 3'UTR of the mouse Col1a1 gene was introduced to (C57BL/6 x 129S4/SvJae)F1-derived v6.5 embryonic stem (ES) cells containing M2-rtTA (optimized rtTA) targeted to the Gt(ROSA)26Sor locus promoter (see Stock No. 006965 for mutation details). Resultant chimeric males were crossed with outbred CD1 females. The Gt(ROSA)26Sor M2-rtTA mutation was bred away from this strain. The line was backcrossed to C57BL/6 for 8 generations by the donating lab.

Control Information

  Control
   Wild-type from the colony
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Related Strains

View Strains carrying other alleles of Col1a1     (18 strains)

Strains carrying other alleles of tetO
008079   129S-Ppargtm2Yba/J
016178   B6(Cg)-Tg(tetO-Cry2)3Jt/J
016176   B6(Cg)-Tg(tetO-Per2)2Jt/J
023757   B6(Cg)-Tg(tetO-tetX,lacZ)1Gogo/UmriJ
009602   B6.129S4(Cg)-Kcnn2tm2Jpad/J
009603   B6.129S4-Kcnn3tm1Jpad/J
023911   B6.Cg-Col1a1tm2(tetO-LIN28B)Gqda/J
023912   B6.Cg-Col1a1tm3(tetO-Mirlet7g/Mir21)Gqda/J
017983   B6.Cg-Col1a1tm9(tetO-Dnmt3b_i1)Jae Gt(ROSA)26Sortm1(rtTA*M2)Jae/J
014588   B6.Cg-Gt(ROSA)26Sortm1(rtTA*M2)Jae Col1a1tm6(tetO-MSI2)Jae/J
023749   B6.Cg-Gt(ROSA)26Sortm1(rtTA*M2)Jae Tg(tetO-Pou5f1,-Sox2,-Klf4,-Myc)1Srn/J
014648   B6.Cg-Gt(ROSA)26Sortm37(H1/tetO-RNAi:Taz)Arte/ZkhuJ
006361   B6.Cg-Tg(Sp7-tTA,tetO-EGFP/cre)1Amc/J
016998   B6.Cg-Tg(TetO-Axin1,EGFP)TA6Cos/J
003762   B6.Cg-Tg(tetFosb)4468Nes/J
007051   B6.Cg-Tg(tetO-APPSwInd)102Dbo/Mmjax
007052   B6.Cg-Tg(tetO-APPSwInd)107Dbo/Mmjax
007049   B6.Cg-Tg(tetO-APPSwInd)885Dbo/Mmjax
007618   B6.Cg-Tg(tetO-Arntl)1Jt/J
017555   B6.Cg-Tg(tetO-CALY)5Cber/J
024114   B6.Cg-Tg(tetO-CHRM4*)2Blr/J
008277   B6.Cg-Tg(tetO-Clockm1Jt)CL57Jt/J
008468   B6.Cg-Tg(tetO-DTA)1Gfi/J
017791   B6.Cg-Tg(tetO-Hamp)2181Nca/J
009344   B6.Cg-Tg(tetO-Ifng)184Pop/J
009136   B6.Cg-Tg(tetO-Kcnj2,lacZ)1Gogo/J
013583   B6.Cg-Tg(tetO-LRRK2)C7874Cai/J
020652   B6.Cg-Tg(tetO-Mif)279Aren/J
017331   B6.Cg-Tg(tetO-Ppp3ca*)11255Kndl/J
017332   B6.Cg-Tg(tetO-Ppp3ca*)13967Kndl/J
017330   B6.Cg-Tg(tetO-TAg*)175Kndl/J
006234   B6.Cg-Tg(tetO-cre)1Jaw/J
005738   B6.FVB-Tg(tetO-EGFP,-Tgfbr2)8Mcle/J
021025   B6;129-Gt(ROSA)26Sortm1(rtTA*M2)Jae Col1a1tm1(tetO-cre)Haho/J
006911   B6;129-Gt(ROSA)26Sortm1(rtTA*M2)Jae Col1a1tm2(tetO-Pou5f1)Jae/J
011001   B6;129S4-Col1a1tm1(tetO-Pou5f1,-Klf4,-Sox2,-Myc)Hoch/J
016836   B6;129S4-Gt(ROSA)26Sortm1(rtTA*M2)Jae Col1a1tm7(tetO-HIST1H2BJ/GFP)Jae/J
012433   B6;C3-Tg(ACTA1-rtTA,tetO-cre)102Monk/J
002709   B6;C3-Tg(TettTALuc)1Dgs/J
023598   B6;C3-Tg(tetO-AIMP2)630Tmd/J
023642   B6;C3-Tg(tetO-AIMP2)634Tmd/J
016841   B6;C3-Tg(tetO-TARDBP)12Vle/J
014650   B6;C3-Tg(tetO-TARDBP*)4Vle/J
012450   B6;D2-Tg(tetO-SNCA)1Cai/J
008344   B6;DBA-Tg(Fos-tTA,Fos-EGFP*)1Mmay Tg(tetO-lacZ,tTA*)1Mmay/J
024742   B6;DBA-Tg(tetO-GCaMP6s)2Niell/J
024088   B6;FVB-Tg(tetO-AML1/ETO)8Dzh/J
008082   B6;SJL-Tg(Tagln-tTA)1Mrab Tg(tetO-Mcpt1)1Mrab/J
010575   B6;SJL-Tg(tetO-Egfr*)2-9Jek/J
010577   B6;SJL-Tg(tetO-Erbb2*)8-4Jek/J
002621   B6;SJL-Tg(tetop-lacZ)2Mam/J
006004   B6C3-Tg(tetO-APPSwInd)885Dbo/Mmjax
016976   B6C3-Tg(tetO-SNCA*A53T)33Vle/J
018913   B6N.Cg-Tg(tetO-GFP,-lacZ)G3Rsp/J
006244   C.Cg-Tg(tetO-cre)1Jaw/J
017719   C3HeB/FeJ-Tg(tetO-TAg)1Efr/J
017955   C57BL/6-Tg(Gfap-rtTA,tetO-MAOB,-lacZ)1Jkan/J
005706   C57BL/6-Tg(tetO-CDK5R1/GFP)337Lht/J
006618   C57BL/6-Tg(tetO-COX8A/EYFP)1Ksn/J
017613   C57BL/6-Tg(tetO-Cdkn1b)1Scpr/J
013729   C57BL/6-Tg(tetO-EDN1,-lacZ)9Mhus/J
016260   C57BL/6-Tg(tetO-Fbxl21)38Jt/J
016179   C57BL/6-Tg(tetO-Fbxl21*)11Jt/J
010713   C57BL/6-Tg(tetO-GFP/tetX)5696Stl/J
013728   C57BL/6-Tg(tetO-NOS2,-lacZ)240iMhus/J
016181   C57BL/6-Tg(tetO-Nr1d1)1Schb/J
016581   C57BL/6J-Tg(tetO-Btrc*)1Jt/J
008278   C57BL/6J-Tg(tetO-Clock)1Jt/J
016580   C57BL/6J-Tg(tetO-Usf1)2Jt/J
021065   FVB(C)-Tg(tetO-Npc1/YFP)1Mps/J
017542   FVB-Tg(Myh6/tetO-ATP2B4)1Jmol/J
016571   FVB-Tg(Myh6/tetO-Gata6)2Jmol/J
014155   FVB-Tg(Myh6/tetO-Itpr1)22.3Jmol/J
014153   FVB-Tg(Myh6/tetO-Itpr2)3.11Jmol/J
014154   FVB-Tg(Myh6/tetO-Itpr2)4.9Jmol/J
012684   FVB-Tg(Myh6/tetO-POSTN)22.1Jmol/J
010580   FVB-Tg(Myh6/tetO-PRKCA*)1Jmk/J
013156   FVB-Tg(tetO-CDK5R1*)1Vln/J
013777   FVB-Tg(tetO-Cacna1g)1Jmol/J
013778   FVB-Tg(tetO-Cacnb2)1Jmol/J
013779   FVB-Tg(tetO-Cacnb2)2Jmol/J
013780   FVB-Tg(tetO-Cib1)1Jmol/J
010578   FVB-Tg(tetO-Dusp6)1Jmol/J
017333   FVB-Tg(tetO-Gnai2*,-lacZ)382Kndl/J
008685   FVB-Tg(tetO-Kdr*)4377.5Rwng/J
023397   FVB-Tg(tetO-Lmnb1)AF1Yfu/J
015815   FVB-Tg(tetO-MAPT*P301L)#Kha/JlwsJ
008695   FVB-Tg(tetO-MET)23Rwng/J
012387   FVB-Tg(tetO-Ppargc1a)1Dpk/J
012385   FVB-Tg(tetO-Ppargc1b)7Dpk/J
022979   FVB-Tg(tetO-Thbs4)17.7Jmol/J
006439   FVB-Tg(tetO/CMV-KRAS*G12C)9.1Msmi/J
019038   FVB.Cg-Tg(tetO-GLI1)10Rup/Mmjax
019039   FVB.Cg-Tg(tetO-KLF4)32831Rup/Mmjax
008244   FVB.Cg-Tg(tetO-cre)1Jaw/J
012459   FVB/N-Tg(Myh6*/tetO-Capn1)L2Gwd/J
005941   FVB/N-Tg(tetO-Aurkb,lacZ)41Kra/J
006202   FVB/N-Tg(tetO-BCR/ABL1)2Dgt/J
014547   FVB/N-Tg(tetO-Fasl)BDepa/J
019376   FVB/N-Tg(tetO-MYC)36aBop/J
022938   FVB/N-Tg(tetO-Wnt5a)17Rva/J
003315   FVB/N-Tg(tetORo1-lacZ)3Conk/J
005076   NOD.Cg-Tg(tetO-EGFP/FADD)1Doi/DoiJ
006999   STOCK Dbttm1Geh Tg(Cebpb-tTA)5Bjd Tg(tetO-DBT)A1Geh/J
011004   STOCK Gt(ROSA)26Sortm1(rtTA*M2)Jae Col1a1tm3(tetO-Pou5f1,-Sox2,-Klf4,-Myc)Jae/J
011011   STOCK Gt(ROSA)26Sortm1(rtTA*M2)Jae Col1a1tm4(tetO-Pou5f1,-Sox2,-Klf4,-Myc)Jae/J
011013   STOCK Gt(ROSA)26Sortm1(rtTA*M2)Jae Col1a1tm5(tetO-Pou5f1,-Klf4,-Myc)Jae/J
018999   STOCK Gt(ROSA)26Sortm1(tTA,tetO-Mir155)Fjsl/J
017596   STOCK Gt(ROSA)26Sortm1.1(rtTA,EGFP)Nagy Tg(SMN2)89Ahmb Smn1tm1Msd Tg(SMN2*delta7)4299Ahmb Tg(tetO-SMN2,-luc)#aAhmb/J
017597   STOCK Gt(ROSA)26Sortm1.1(rtTA,EGFP)Nagy Tg(SMN2)89Ahmb Smn1tm1Msd Tg(SMN2*delta7)4299Ahmb Tg(tetO-SMN2,-luc)#bAhmb/J
024854   STOCK Tg(Camk2a-tTA)1Mmay Tg(tetO-MAPT*P301L)#Kha/J
008755   STOCK Tg(Ins2-rtTA)2Efr Tg(teto-DTA)1Gfi/J
012477   STOCK Tg(Myh6*/tetO-GCaMP2)1Mik/J
016572   STOCK Tg(Myh6/tetO-Gata4)1Jmol/J
014544   STOCK Tg(tetO-ABL1*P242E*P249E)CPdav/J
014093   STOCK Tg(tetO-CHRM3*)1Blr/J
008790   STOCK Tg(tetO-DISC1*)1001Plet/J
008168   STOCK Tg(tetO-DTA)1Gfi/J
017755   STOCK Tg(tetO-GCAMP2)12iRyu/J
024509   STOCK Tg(tetO-Gata6)1Abl/J
016970   STOCK Tg(tetO-HCV)1Mlch/Mmjax
005104   STOCK Tg(tetO-HIST1H2BJ/GFP)47Efu/J
005699   STOCK Tg(tetO-Ipf1,EGFP)956.6Macd/J
005728   STOCK Tg(tetO-Ipf1,lacZ)958.1Macd/J
012441   STOCK Tg(tetO-LRRK2*G2019S)E3Cai/J
017918   STOCK Tg(tetO-MAML1*/EGFP)2Akar/J
017599   STOCK Tg(tetO-SMN2,-luc)#aAhmb/J
017600   STOCK Tg(tetO-SMN2,-luc)#bAhmb/J
012442   STOCK Tg(tetO-SNCA*A53T)E2Cai/J
006224   STOCK Tg(tetO-cre)1Jaw/J
017906   STOCK Tg(tetO-hop/EGFP,-COP4/mCherry)6Kftnk/J
012345   STOCK Tg(tetO-tdTomato,-Syp/EGFP*)1.1Luo/J
012449   STOCK Tg(teto-LRRK2)C7874Cai/J
View Strains carrying other alleles of tetO     (133 strains)

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested.
Caffey Disease   (COL1A1)
Collagen, Type I, Alpha-1; COL1A1   (COL1A1)
Ehlers-Danlos Syndrome, Type I   (COL1A1)
Ehlers-Danlos Syndrome, Type VII, Autosomal Dominant   (COL1A1)
Osteogenesis Imperfecta, Type I   (COL1A1)
Osteogenesis Imperfecta, Type II   (COL1A1)
Osteogenesis Imperfecta, Type III   (COL1A1)
Osteogenesis Imperfecta, Type IV   (COL1A1)
Osteoporosis   (COL1A1)
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Col1a1tm1(tetO-Lin28a)Gqda/Col1a1+

        involves: 129S4/SvJae * C57BL/6 * CD-1
  • mortality/aging
  • premature death
    • following induction with doxycycline associated with marked gut pathology   (MGI Ref ID J:166568)
  • reproductive system phenotype
  • *normal* reproductive system phenotype
    • despite delayed sexual maturation in female mice, fertility over the first 3 months of life is normal   (MGI Ref ID J:166568)
    • delayed female fertility
      • time to first litter is delayed compared with wild-type mice   (MGI Ref ID J:166568)
      • however, timing of first estrus is normal   (MGI Ref ID J:166568)
    • delayed sexual maturation
      • puberty is delayed in female mice compared with wild-type mice   (MGI Ref ID J:166568)
      • at P26, uterine plus ovarian mass is less than in wild-type mice indicating delayed sexual development   (MGI Ref ID J:166568)
      • however, timing of first estrus is normal   (MGI Ref ID J:166568)
    • delayed vaginal opening   (MGI Ref ID J:166568)
    • increased litter size
      • in the first litter   (MGI Ref ID J:166568)
  • growth/size/body phenotype
  • abnormal facial morphology
    • mice exhibit wider facies compared with wild-type mice   (MGI Ref ID J:166568)
  • big ears   (MGI Ref ID J:166568)
  • decreased susceptibility to age related obesity
    • gain less fat mass with age compared to wild-type mice on a normal diet   (MGI Ref ID J:177113)
  • decreased susceptibility to diet-induced obesity
    • gain less fat mass when fed a high fat diet compared to wild-type mice   (MGI Ref ID J:177113)
    • no difference in the consumption of high fat food is detected   (MGI Ref ID J:177113)
  • decreased total body fat amount
    • decrease in the percentage of body fat   (MGI Ref ID J:177113)
  • increased body size
    • regardless of doxycycline induction   (MGI Ref ID J:166568)
    • increased body length
      • after weaning   (MGI Ref ID J:166568)
    • increased body weight
      • after weaning   (MGI Ref ID J:166568)
  • increased growth rate   (MGI Ref ID J:166568)
  • increased lean body mass
    • increase in the percentage of lean mass   (MGI Ref ID J:177113)
  • homeostasis/metabolism phenotype
  • decreased circulating glucose level
    • in fed and fasted states   (MGI Ref ID J:166568)
  • decreased circulating insulin level
    • after glucose stimulation   (MGI Ref ID J:166568)
  • decreased susceptibility to diet-induced obesity
    • gain less fat mass when fed a high fat diet compared to wild-type mice   (MGI Ref ID J:177113)
    • no difference in the consumption of high fat food is detected   (MGI Ref ID J:177113)
  • improved glucose tolerance
    • without increased insulin secretion   (MGI Ref ID J:166568)
    • under high fat diet conditions   (MGI Ref ID J:177113)
  • increased insulin sensitivity   (MGI Ref ID J:166568)
    • under high fat diet conditions   (MGI Ref ID J:177113)
  • skeleton phenotype
  • abnormal skeleton morphology
    • mice exhibit large bones compared with wild-type mice   (MGI Ref ID J:166568)
    • mice exhibit bone hyperplasia compared with wild-type mice   (MGI Ref ID J:166568)
    • increased bone mineral density
      • bone mineral content and density are increased compared to in wild-type mice   (MGI Ref ID J:166568)
  • craniofacial phenotype
  • abnormal facial morphology
    • mice exhibit wider facies compared with wild-type mice   (MGI Ref ID J:166568)
  • big ears   (MGI Ref ID J:166568)
  • liver/biliary system phenotype
  • decreased susceptibility to hepatic steatosis
    • under high fat diet conditions   (MGI Ref ID J:177113)
  • increased hepatocyte proliferation
    • mice exhibit an increase in cell number compared with wild-type mice without a change in cell size   (MGI Ref ID J:166568)
  • liver hyperplasia
    • mice exhibit an increase in cell number compared with wild-type mice without a change in cell size   (MGI Ref ID J:166568)
  • cellular phenotype
  • increased cell proliferation
    • doxycycline-treated mice exhibit an increase in cell proliferation in the intestinal crypts, blood, and skin compared with wild-type mice   (MGI Ref ID J:166568)
    • mice exhibit cell hyperplasia without an increase in cell size   (MGI Ref ID J:166568)
    • increased hepatocyte proliferation
      • mice exhibit an increase in cell number compared with wild-type mice without a change in cell size   (MGI Ref ID J:166568)
  • digestive/alimentary phenotype
  • abnormal digestive system morphology
    • following induction with doxycycline, mice exhibit marked gut pathology unlike wild-type mice   (MGI Ref ID J:166568)
  • hearing/vestibular/ear phenotype
  • big ears   (MGI Ref ID J:166568)
  • endocrine/exocrine gland phenotype
  • small pancreatic islets   (MGI Ref ID J:166568)
  • integument phenotype
  • coarse hair   (MGI Ref ID J:166568)
  • skin hyperplasia   (MGI Ref ID J:166568)
  • thick skin   (MGI Ref ID J:166568)
  • adipose tissue phenotype
  • decreased total body fat amount
    • decrease in the percentage of body fat   (MGI Ref ID J:177113)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Developmental Biology Research
Growth Defects

Diabetes and Obesity Research
Obesity Without Diabetes
      diet-induced, resistant

Reproductive Biology Research
Fertility Defects

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Col1a1tm1(tetO-Lin28a)Gqda
Allele Name targeted mutation 1, George Daley
Allele Type Targeted (Inducible, Inserted expressed sequence)
Common Name(s) Lin28a Tg;
Strain of Origin(C57BL/6 x 129S4/SvJae)F1
Expressed Gene tetO, tet operator,
General Note The allele was made in KH2 cells that carry Gt(ROSA)26Sortm1(rtTA*M2)Jae and Col1a1tm13(neo/hygro*)Jae.
Molecular Note RMCE on KH2 cells inserted a cassette that contains a splice acceptor, 2 polyadenylation sequence, tetracycline operator, flag-tagged mouse cDNA, and an SV40 polyadenylation signal into the 3'UTR. Leaky ectopic expression in the absence of doxycycline induction was confirmed by quantitative RT-PCR and immunohistochemistry. [MGI Ref ID J:166568]
 
Gene Symbol and Name Col1a1, collagen, type I, alpha 1
Chromosome 11
Gene Common Name(s) COLIA1; Col1a-1; Cola-1; Cola1; Moloney leukemia virus 13; Mov-13; OI4;

Genotyping

Genotyping Information

Genotyping Protocols

Col1a1tm1(tetO-Lin28a)Gqda, Separated PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Zhu H; Shah S; Shyh-Chang N; Shinoda G; Einhorn WS; Viswanathan SR; Takeuchi A; Grasemann C; Rinn JL; Lopez MF; Hirschhorn JN; Palmert MR; Daley GQ. 2010. Lin28a transgenic mice manifest size and puberty phenotypes identified in human genetic association studies. Nat Genet 42(7):626-30. [PubMed: 20512147]  [MGI Ref ID J:166568]

Additional References

Col1a1tm1(tetO-Lin28a)Gqda related

Kasinski AL; Slack FJ. 2011. Epigenetics and genetics. MicroRNAs en route to the clinic: progress in validating and targeting microRNAs for cancer therapy. Nat Rev Cancer 11(12):849-64. [PubMed: 22113163]  [MGI Ref ID J:178933]

Nishino J; Kim S; Zhu Y; Zhu H; Morrison SJ. 2013. A network of heterochronic genes including Imp1 regulates temporal changes in stem cell properties. Elife 2:e00924. [PubMed: 24192035]  [MGI Ref ID J:207231]

Shyh-Chang N; Zhu H; Yvanka de Soysa T; Shinoda G; Seligson MT; Tsanov KM; Nguyen L; Asara JM; Cantley LC; Daley GQ. 2013. Lin28 enhances tissue repair by reprogramming cellular metabolism. Cell 155(4):778-92. [PubMed: 24209617]  [MGI Ref ID J:205289]

Zhu H; Shyh-Chang N; Segre AV; Shinoda G; Shah SP; Einhorn WS; Takeuchi A; Engreitz JM; Hagan JP; Kharas MG; Urbach A; Thornton JE; Triboulet R; Gregory RI; Altshuler D; Daley GQ. 2011. The Lin28/let-7 Axis Regulates Glucose Metabolism. Cell 147(1):81-94. [PubMed: 21962509]  [MGI Ref ID J:177113]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX12

Colony Maintenance

Breeding & HusbandryHomozygous and heterozygous tetO-Lin28a are viable and fertile.
Mating SystemHeterozygote x Heterozygote         (Female x Male)   09-MAY-14

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


 

This strain is currently Under Development - Now Accepting Orders.
Estimated Available for Distribution Date: 27-OCT-14

Please note: You may now place orders for this strain although it is not yet ready for distribution. Estimated available for distribution dates are provided to keep customers better informed on strains under development. Please note that our Colony Managers routinely monitor the target date and edit it based on breeding performance and other factors. The length of time it takes to make a new strain available for distribution depends on genotype, age, number of animals sent by the Donating Investigator, breeding performance, additional strain development (backcrossing, making homozygous), and anticipated demand for the strain.

Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $232.00Female or MaleHeterozygous for Col1a1tm1(tetO-Lin28a)Gqda  
$232.00Female or MaleHomozygous for Col1a1tm1(tetO-Lin28a)Gqda  
Price per Pair (US dollars $)Pair Genotype
$464.00Heterozygous for Col1a1tm1(tetO-Lin28a)Gqda x Heterozygous for Col1a1tm1(tetO-Lin28a)Gqda  

Standard Supply

Under Development - Now Accepting Orders The strain development process (i.e. importation, rederivation, and colony expansion) usually takes six to nine months.

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $301.60Female or MaleHeterozygous for Col1a1tm1(tetO-Lin28a)Gqda  
$301.60Female or MaleHomozygous for Col1a1tm1(tetO-Lin28a)Gqda  
Price per Pair (US dollars $)Pair Genotype
$603.20Heterozygous for Col1a1tm1(tetO-Lin28a)Gqda x Heterozygous for Col1a1tm1(tetO-Lin28a)Gqda  

Standard Supply

Under Development - Now Accepting Orders The strain development process (i.e. importation, rederivation, and colony expansion) usually takes six to nine months.

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Standard Supply

Under Development - Now Accepting Orders The strain development process (i.e. importation, rederivation, and colony expansion) usually takes six to nine months.

Control Information

  Control
   Wild-type from the colony
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.


See Terms of Use tab for General Terms and Conditions


The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
Ordering Information
JAX® Mice
Surgical and Preconditioning Services
JAX® Services
Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form

Terms of Use

Terms of Use


General Terms and Conditions


For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.

Contact information

General inquiries regarding Terms of Use

Contracts Administration

phone:207-288-6470

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


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