Strain Name:

B6.Cg-Lin28atm1.2Gqda/J

Stock Number:

023913

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Availability:

Under Development - Now Accepting Orders

Estimated Available for Distribution Date: 12-MAY-14
Use Restrictions Apply, see Terms of Use
Exon 2 of the Lin28a (lin-28 homolog A (C. elegans)) gene is flanked by loxP sites in this conditional targeted mutation strain. Cre excision of the floxed segment produces a knockout allele.

Description

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Mating SystemHeterozygote x Heterozygote         (Female x Male)   02-JAN-14
Specieslaboratory mouse
GenerationN6+pN1 (28-FEB-14)
Generation Definitions
 
Donating Investigator George Q. Daley,   Children's Hospital Boston

Description
LIN28A (lin-28 homolog A (C. elegans)) is an RNA-binding protein that blocks biogenesis of Mirlet7 (let-7) microRNAs. Let-7 targets are known regulators of mammalian body size and metabolism.

Exon 2 of the gene is flanked by loxP sites in this conditional targeted mutation strain. Cre excision of the floxed segment produces a knockout allele.

Muscle-specific knockout mice created through crosses with Myf5 (myogenic factor 5)-cre animals display glucose intolerance and reduced Insr (insulin receptor) expression.

Mice created from crosses with germline-specific Ddx4-cre mice (see Stock No. 006954) show dwarfism as early as E13.5 and by E18.5 show 20% reduction in weight relative to heterozygous controls. Although born at expected Mendelian ratios, roughly 93% of Lin28a knockout mice die within 1 day of birth (2 out of 5 mice harboring a cardiac ventricular septal defect). Those animals that survive are 30-50% smaller in weight and 20-30% shorter in length than controls. Dual-energy x-ray absorptiometry imaging confirms the decreased fat mass and also revealed decreased bone mineral density in the knockout animals, suggesting that these animals suffer from metabolic dysregulation.

Development
Exon 2 was flanked by loxP sites and an FRT-flanked neomycin cassette was introduced through homologous recombination in (C57BL/6 x 129S4/SvJae)F1-derived v6.5 embryonic stem (ES) cells. The neomycin cassette was excised via a Flp recombinase. This strain was backcrossed to C57BL/6 for 6 generations by the donating lab.

Control Information

  Control
   Wild-type from the colony
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Lin28a
023915   B6.Cg-Lin28atm2.1Gqda Lin28btm2.1Gqda/J
023916   FVB.Cg-Lin28atm1.1Gqda/J
View Strains carrying other alleles of Lin28a     (2 strains)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Lin28atm1.2Gqda/Lin28atm1.2Gqda

        B6.Cg-Lin28atm1.2Gqda/J
  • normal phenotype
  • no abnormal phenotype detected   (MGI Ref ID J:101977)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Research Tools
Cre-lox System
      loxP-flanked Sequences
Diabetes and Obesity Research
      loxP

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Lin28atm1.2Gqda
Allele Name targeted mutation 1.2, George Q Daley
Allele Type Targeted (Floxed/Frt)
Strain of Origin(C57BL/6 x 129S4/SvJae)F1
Gene Symbol and Name Lin28a, lin-28 homolog A (C. elegans)
Chromosome 4
Gene Common Name(s) AL024421; CSDD1; LIN-28; LIN28; Lin28; RGD1566408; Tex17; ZCCHC1; expressed sequence AL024421; lin-28 homolog (C. elegans); lin-28A; testis expressed gene 17;
Molecular Note A loxP site was inserted upstream of exon 2. An FRT flanked neo cassette with a 5' loxP site was inserted downstream of exon 2 via homologous recombination. FLP-mediated recombination removed the neomycin cassette. [MGI Ref ID J:101977] [MGI Ref ID J:177113]

Genotyping

Genotyping Information

Genotyping Protocols

Lin28atm1.2Gqda, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Zhu H; Shyh-Chang N; Segre AV; Shinoda G; Shah SP; Einhorn WS; Takeuchi A; Engreitz JM; Hagan JP; Kharas MG; Urbach A; Thornton JE; Triboulet R; Gregory RI; Altshuler D; Daley GQ. 2011. The Lin28/let-7 Axis Regulates Glucose Metabolism. Cell 147(1):81-94. [PubMed: 21962509]  [MGI Ref ID J:177113]

Additional References

Lin28atm1.2Gqda related

The Jackson Laboratory. 2005. Information obtained from The Jackson Laboratory, Bar Harbor, ME Unpublished :.  [MGI Ref ID J:101977]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX18

Colony Maintenance

Breeding & HusbandryHomozygous and heterozygous floxed mice are viable and fertile.
Mating SystemHeterozygote x Heterozygote         (Female x Male)   02-JAN-14

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


 

This strain is currently Under Development - Now Accepting Orders.
Estimated Available for Distribution Date: 12-MAY-14

Please note: You may now place orders for this strain although it is not yet ready for distribution. Estimated available for distribution dates are provided to keep customers better informed on strains under development. Please note that our Colony Managers routinely monitor the target date and edit it based on breeding performance and other factors. The length of time it takes to make a new strain available for distribution depends on genotype, age, number of animals sent by the Donating Investigator, breeding performance, additional strain development (backcrossing, making homozygous), and anticipated demand for the strain.

Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $232.00Female or MaleHeterozygous for Lin28atm1.2Gqda  
$232.00Female or MaleHomozygous for Lin28atm1.2Gqda  
Price per Pair (US dollars $)Pair Genotype
$464.00Heterozygous for Lin28atm1.2Gqda x Heterozygous for Lin28atm1.2Gqda  

Standard Supply

Under Development - Now Accepting Orders The strain development process (i.e. importation, rederivation, and colony expansion) usually takes six to nine months.

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $301.60Female or MaleHeterozygous for Lin28atm1.2Gqda  
$301.60Female or MaleHomozygous for Lin28atm1.2Gqda  
Price per Pair (US dollars $)Pair Genotype
$603.20Heterozygous for Lin28atm1.2Gqda x Heterozygous for Lin28atm1.2Gqda  

Standard Supply

Under Development - Now Accepting Orders The strain development process (i.e. importation, rederivation, and colony expansion) usually takes six to nine months.

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Under Development - Now Accepting Orders The strain development process (i.e. importation, rederivation, and colony expansion) usually takes six to nine months.

Control Information

  Control
   Wild-type from the colony
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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