Strain Name:

STOCK Lin28btm1.2Gqda/J

Stock Number:

023914

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Availability:

Cryopreserved - Ready for recovery

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Exon 2 of the Lin28b (lin-28 homolog B (C. elegans)) gene, encoding the functional cold shock domain, was flanked by loxP sites in this conditional mutation strain. Cre-mediated excision of the floxed segment creates a knockout allele.

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names B6.Cg-Lin28btm1.2Gqda/J    (Changed: 19-JUN-14 )
Type Mutant Stock; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Specieslaboratory mouse
Generation+pN1
Generation Definitions
 
Donating Investigator George Q. Daley,   Children's Hospital Boston

Description
Lin28b (lin-28 homolog B (C. elegans)) is highly expressed during normal embryogenesis and is upregulated in some cancers to potently and selectively block the maturation of let-7 (Mirlet7) tumor suppressor microRNA's.

Exon 2 of the gene, encoding the functional cold shock domain, was flanked by loxP sites in this conditional mutation strain. Cre-mediated excision of the floxed segment creates a knockout allele.

Male (but not female) knockout mice created through crosses with germline-specific Ddx4-cre mice (e.g. Stock No. 006954) show postnatal dwarfism and reduced organ weights.

Skeletal muscle-directed knockouts of Lin28b, created through crosses with Myf5 (myogenic factor 5)-cre mice, produce males with phenotypic dwarfism that recapitulates that of the germline deletion. Muscle-specific loss of LIN28B leads to insulin resistance and impaired glucose uptake.

Development
Exon 2, encoding the functional cold shock domain, was flanked by loxP sites and an FRT-flanked PGK-neomycin cassette was introduced to intron 2. The mutation was created in (C57BL/6 x 129S4/SvJae)F1-derived v6.5 embryonic stem (ES) cells. Chimeric males were bred to BALB/c females. The neomycin cassette was excised via a Flp recombinase. This strain was backcrossed to C57BL/6 for 6 generations by the donating lab (see SNP note below).

A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. Eight of the 27 markers throughout the genome were segregating suggested an incomplete backcross. Also, 2 of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a mixed C57BL/6J ; C57BL/6N genetic.

Control Information

  Control
   Wild-type from the colony
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Lin28b
023917   B6.Cg-Lin28btm1.1Gqda/J
025324   C57BL/6N-Lin28btm1a(EUCOMM)Hmgu/J
023915   STOCK Lin28atm2.1Gqda Lin28btm2.1Gqda/J
View Strains carrying other alleles of Lin28b     (3 strains)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Lin28btm1.2Gqda/Lin28btm1.2Gqda

        STOCK Lin28btm1.2Gqda/J
  • normal phenotype
  • no abnormal phenotype detected   (MGI Ref ID J:101977)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Research Tools
Cre-lox System
      loxP-flanked Sequences
Developmental Biology Research
      Cre-lox System
Diabetes and Obesity Research
      loxP

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Lin28btm1.2Gqda
Allele Name targeted mutation 1.2, George Q Daley
Allele Type Targeted (Conditional ready (e.g. floxed), No functional change)
Strain of Origin(C57BL/6 x 129S4/SvJae)F1
Gene Symbol and Name Lin28b, lin-28 homolog B (C. elegans)
Chromosome 10
Gene Common Name(s) 2810403D23Rik; CSDD2; D030047M17Rik; RIKEN cDNA 2810403D23 gene; RIKEN cDNA D030047M17 gene;
Molecular Note A loxP site was inserted upstream of exon 2. An FRT-flanked neomycin resistance cassette with a 5' loxP site was inserted downstream of exon 2. FLP-mediated recombination removed the neomycin cassette. [MGI Ref ID J:101977] [MGI Ref ID J:199720]

Genotyping

Genotyping Information

Genotyping Protocols

Lin28btm1.2Gqda, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Shinoda G; Shyh-Chang N; de Soysa TY; Zhu H; Seligson MT; Shah SP; Abo-Sido N; Yabuuchi A; Hagan JP; Gregory RI; Asara JM; Cantley LC; Moss EG; Daley GQ. 2013. Fetal Deficiency of Lin28 Programs Life-Long Aberrations in Growth and Glucose Metabolism. Stem Cells :. [PubMed: 23666760]  [MGI Ref ID J:199720]

Additional References

Lin28btm1.2Gqda related

The Jackson Laboratory. 2005. Information obtained from The Jackson Laboratory, Bar Harbor, ME Unpublished :.  [MGI Ref ID J:101977]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryHomozygous and heterozygous floxed mice are viable and fertile.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2140.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2782.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   Wild-type from the colony
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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