Strain Name:

FVB.Cg-Lin28btm1.1Gqda/J

Stock Number:

023917

Availability:

Awaiting Transfer from the Donor

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Lin28b (lin-28 homolog B (C. elegans)) knockout males, but not females, show postnatal dwarfism.

Description

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation;
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Specieslaboratory mouse
 
Donating Investigator George Q. Daley,   Children's Hospital Boston

Description
Lin28b (lin-28 homolog B (C. elegans)) has been implicated with human growth and glucose metabolism.

Exon 2 of the Lin28b mouse gene, encoding the functional cold shock domain, was deleted in this targeted mutation strain. The weights of homozygous embryos and their placentae at E18.5 are comparable to those of heterozygotes, suggesting the gene is dispensible for embryonic growth. Males show postnatal dwarfism and organ weights are reduced in relative proportion to total body weight. Females show no postnatal growth defects.

Development
A targeting vector was used to introduce loxP sites on either side of exon 2 (encoding the functional cold shock domain) and an FRT-flanked PGK-neomycin cassette to intron 2 via homologous recombination in (C57BL/6 x 129S4/SvJae)F1-derived v6.5 embryonic stem (ES) cells. Chimeric males were bred to BALB/c females, then the line was backcrossed to C57BL/6 for 7 generations. Mice were crossed with FVB background Ddx4-cre mice (see Stock No. 006954) to excise the floxed region. This strain was then backcrossed to FVB for 5 generations by the donating laboratory.

Control Information

  Control
   Wild-type from the colony
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Lin28b
023915   B6.Cg-Lin28atm2.1Gqda Lin28btm2.1Gqda/J
023914   B6.Cg-Lin28btm1.2Gqda/J
View Strains carrying other alleles of Lin28b     (2 strains)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.

Lin28btm1.1Gqda/Lin28btm1.1Gqda

        involves: 129S4/SvJae * BALB/c * C57BL/6 * FVB
  • growth/size/body phenotype
  • decreased body size
    • postnatal dwarfism in male, but not female, mice   (MGI Ref ID J:199720)

The following phenotype relates to a compound genotype created using this strain.
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Lin28btm1Gqda/Lin28btm1.1Gqda Tg(UBC-cre/ERT2)1Ejb/0

        involves: 129S/SvEv * 129S4/SvJae * C57BL/6   (conditional)
  • growth/size/body phenotype
  • *normal* growth/size/body phenotype
    • mice exhibit normal growth when treated with tamoxifen at 7 to 9 days or 6 weeks of age   (MGI Ref ID J:199720)
    • decreased body size
      • postnatal dwarfism in male, but not female, mice treated with tamoxifen at E15.5   (MGI Ref ID J:199720)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Developmental Biology Research
Growth Defects
      Growth Defects (homozygous)

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Lin28btm1.1Gqda
Allele Name targeted mutation 1.1, George Q Daley
Allele Type Targeted (knock-out)
Strain of Origin(C57BL/6 x 129S4/SvJae)F1
Gene Symbol and Name Lin28b, lin-28 homolog B (C. elegans)
Chromosome 10
Gene Common Name(s) 2810403D23Rik; CSDD2; D030047M17Rik; RIKEN cDNA 2810403D23 gene; RIKEN cDNA D030047M17 gene;
Molecular Note A loxP site was inserted upstream of exon 2. An FRT-flanked neomycin resistance cassette with a 5' loxP site was inserted downstream of exon 2. Cre-mediated recombination removed exon 2. Western blot analysis confirmed the absence of protein expression in embryonic stem cells. [MGI Ref ID J:199720]

Genotyping

Genotyping Information


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Shinoda G; Shyh-Chang N; de Soysa TY; Zhu H; Seligson MT; Shah SP; Abo-Sido N; Yabuuchi A; Hagan JP; Gregory RI; Asara JM; Cantley LC; Moss EG; Daley GQ. 2013. Fetal Deficiency of Lin28 Programs Life-Long Aberrations in Growth and Glucose Metabolism. Stem Cells :. [PubMed: 23666760]  [MGI Ref ID J:199720]

Health & husbandry

Health & Colony Maintenance Information

Colony Maintenance

Breeding & HusbandryHeterozgyotes are viable and fertile.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


 

This strain is currently Awaiting Transfer from the Donor.

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Control Information

  Control
   Wild-type from the colony
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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