Strain Name:

B6.129(Cg)-Gpx1tm1Ysh/MgoldJ

Stock Number:

023919

Order this mouse

Availability:

Repository- Live

This Gpx1 knockout strain is useful in studies of redox-mediated signaling and oxidative stress.

Description

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Mating SystemHeterozygote x Heterozygote         (Female x Male)   06-MAR-14
Specieslaboratory mouse
GenerationN9+pN2 (06-MAR-14)
Generation Definitions
 
Donating Investigator Matthew Goldberg,   University of Texas Southwestern Medical Center at Dallas

Description
Gpx1 encodes an intracellular selenium-containing glutathione peroxidase that detoxifies hydrogen peroxide and is involved in regulation of redox-balance and redox-mediated signaling as well as protection against reactive oxygen species. These mice carry a targeted mutation in which exon 2 is replaced by a NEO cassette. Mice that are homozygous for this targeted mutation are viable and fertile. No gene product (mRNA) is detected by Northern blot analysis of brain, heart, kidney, liver, and lung tissue from homozygous mice. Glutathione peroxidase activity is not detected in brain tissue and is significantly reduced in lens, heart, liver and small intestine. Homozygotes exhibit increased sensitivity to noise, with 4 week old mutant mice exhibiting more severe cochlear hair cell and nerve fiber loss after noise exposure than wildtype controls. Reactive oxygen species levels are increased in the brains of knock out mice, which are also resistant to kainic acid induced seizures and neuron damage. Knock out mice are less susceptible to striatal dopaminergic nerve injury after 1-methyl-4-phenyl-1,2,5,6-tetrahydropyridine (MPTP) treatment. Small intestine jejunum crypts regeneration is increased and ileal and jeunum crypts are more resistant to gamma radiation induced damage than wildtype controls. Knock out mice are more susceptible to myocarditis caused by coxsackievirus B3 infection. Arachidonic acid metabolism in mutant mice is impaired in platelets, which when incubated with arachidonic acid, produce decreased levels of 12-hydroxyeicosatetraenoic acid and more polar products when compared to wildtype controls. By 3 to 4 months of age, 13% of homozygous mice on the 129 background develop cataracts. During backcrossing, the Y chromosome may not have been fixed to the C57BL/6J genetic background.

Development
A targeting vector designed by Dr. Ye-Shih Ho (Wayne State University) containing a NEO cassette was used to disrupt exon 2. The construct was electroporated into 129 derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric animals were tested for germline transmission. The mice were crossed for approximately 6 generations to C57BL/6. Dr. Matthew Goldberg obtained the mice and backcrossed them to C57BL/6J for 3 or 4 generations. During backcrossing, the Y chromosome may not have been fixed to the C57BL/6 genetic background. The mice were bred to two other targeted mutant lines on the congenic C57BL/6 background, with some 129 contribution. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to obtain this single mutant line.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Related Strains

Strains carrying   Gpx1tm1Ysh allele
023968   B6.Cg-Park7tm1Shn Gpx1tm1Ysh Park2tm1Shn/MgoldJ
View Strains carrying   Gpx1tm1Ysh     (1 strain)

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested.
Glutathione Peroxidase Deficiency; GPXD   (GPX1)
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Gpx1tm1Ysh/Gpx1tm1Ysh

        B6.129-Gpx1tm1Ysh
  • digestive/alimentary phenotype
  • abnormal small intestine crypts of Lieberkuhn morphology
    • the jejunum crypt, but not ileal crypts, are more resistant to gamma radiation induced damage than controls   (MGI Ref ID J:63988)
  • cellular phenotype
  • decreased cellular sensitivity to gamma-irradiation
    • the jejunum crypt, but not ileal crypts, are more resistant to gamma radiation induced damage than controls   (MGI Ref ID J:63988)
  • endocrine/exocrine gland phenotype
  • abnormal small intestine crypts of Lieberkuhn morphology
    • the jejunum crypt, but not ileal crypts, are more resistant to gamma radiation induced damage than controls   (MGI Ref ID J:63988)

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Gpx1tm1Ysh/Gpx1+

        involves: 129S1/Sv * 129X1/SvJ
  • homeostasis/metabolism phenotype
  • abnormal enzyme/coenzyme activity
    • heterozygous mice show a 40-50% reduction is Gpx1 activity in various tissues compared to wild-type   (MGI Ref ID J:110762)

Gpx1tm1Ysh/Gpx1tm1Ysh

        involves: 129S1/Sv * 129X1/SvJ * C57BL/6J
  • digestive/alimentary phenotype
  • abnormal small intestine crypts of Lieberkuhn morphology
    • ileal crypts and the jejunum crypt are more resistant to gamma radiation induced damage than controls   (MGI Ref ID J:63988)
  • cellular phenotype
  • decreased cellular sensitivity to gamma-irradiation
    • ileal crypts and the jejunum crypt are more resistant to gamma radiation induced damage than controls   (MGI Ref ID J:63988)
  • hematopoietic system phenotype
  • abnormal platelet physiology
    • platelets incubated with arachidonic acid generate less 12-hydroxyeicosatetraenoic acid and more polar products relative to control platelets at a higher concentration of arachidonic acid, however homozygotes are healthy and fertile, show no increase in sensitivity to hyperoxia, have no abnormalities in the brain, heart, intestine, kidney, liver, lung, and spleen, and have normal numbers of total blood cells, red cells, reticulocytes, lymphocytes, monocytes, neutrophils, eosinophils, and platelets   (MGI Ref ID J:41169)
  • homeostasis/metabolism phenotype
  • abnormal platelet physiology
    • platelets incubated with arachidonic acid generate less 12-hydroxyeicosatetraenoic acid and more polar products relative to control platelets at a higher concentration of arachidonic acid, however homozygotes are healthy and fertile, show no increase in sensitivity to hyperoxia, have no abnormalities in the brain, heart, intestine, kidney, liver, lung, and spleen, and have normal numbers of total blood cells, red cells, reticulocytes, lymphocytes, monocytes, neutrophils, eosinophils, and platelets   (MGI Ref ID J:41169)
  • endocrine/exocrine gland phenotype
  • abnormal small intestine crypts of Lieberkuhn morphology
    • ileal crypts and the jejunum crypt are more resistant to gamma radiation induced damage than controls   (MGI Ref ID J:63988)
  • hearing/vestibular/ear phenotype
  • cochlear inner hair cell degeneration
    • at 4 weeks after noise exposure, homozygotes display a more severe IHC loss in the most basal 40% of the cochlea than wild-type mice   (MGI Ref ID J:113167)
  • cochlear outer hair cell degeneration
    • at 4 weeks after noise exposure, homozygotes display a more severe OHC loss in the most basal 40% of the cochlea than wild-type mice   (MGI Ref ID J:113167)
  • increased or absent threshold for auditory brainstem response
    • prior to noise exposure, 2-month-old homozygotes exhibit significantly higher ABR thresholds only at 28.3 kHz (16.4 dB), but not at 5 kHz (1.3 dB), 10 kHz (0.3 dB) or 20 kHz (8.3 dB), relative to wild-type mice   (MGI Ref ID J:113167)
  • increased susceptibility to noise-induced hearing loss
    • at 4 weeks after broadband noise exposure (110 dB SPL; 1 hr), 2-month-old homozygotes display up to 15 dB higher ABR thresholds than wild-type mice across all test frequencies, with significant ABR elevations at 20 kHz (~23 db) and 28.3 kHz (~15 dB)   (MGI Ref ID J:113167)
    • the contribution of null genotype to noise-induced hearing loss ranges from ~0 dB at 5 kHz to an estimated 15 dB at 20 kHz   (MGI Ref ID J:113167)
  • nervous system phenotype
  • cochlear ganglion degeneration
    • at 4 weeks after noise exposure, homozygotes exhibit a ~50% greater loss of nerve fibers per habenula in the cochlear base than wild-type mice   (MGI Ref ID J:113167)
  • cochlear inner hair cell degeneration
    • at 4 weeks after noise exposure, homozygotes display a more severe IHC loss in the most basal 40% of the cochlea than wild-type mice   (MGI Ref ID J:113167)
  • cochlear outer hair cell degeneration
    • at 4 weeks after noise exposure, homozygotes display a more severe OHC loss in the most basal 40% of the cochlea than wild-type mice   (MGI Ref ID J:113167)

Gpx1tm1Ysh/Gpx1tm1Ysh

        involves: 129S1/Sv * 129X1/SvJ
  • homeostasis/metabolism phenotype
  • abnormal catalase activity
    • catalase activity varies considerably among tissues examined, from only 6% of the Gpx1 activity in the lens to 222% in the liver   (MGI Ref ID J:110762)
    • the lens has the lowest Gpx1 and catalase activities of any tissue based on activity per mg of total lens protein   (MGI Ref ID J:110762)
  • decreased glutathione peroxidase activity
    • glutathione peroxidase activity is very low or undetectable in mutants in the lens or other tissues   (MGI Ref ID J:110762)
    • the lens has the lowest Gpx1 and catalase activities of any tissue based on activity per mg of total lens protein   (MGI Ref ID J:110762)
  • vision/eye phenotype
  • cataracts
    • 13% of mice develop cataracts between 3 and 4 months of age   (MGI Ref ID J:142446)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Cell Biology Research
Signal Transduction

Neurobiology Research
Vestibular Defects

Research Tools
Cell Biology Research

Sensorineural Research
Cataracts
Vestibular Defects

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Gpx1tm1Ysh
Allele Name targeted mutation 1, Ye-Shih Ho
Allele Type Targeted (Null/Knockout)
Common Name(s) GPX1 KO; GSHPx-1-deficient; GpX-1 KO; Gpx1-;
Strain of Origin(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
Gene Symbol and Name Gpx1, glutathione peroxidase 1
Chromosome 9
Gene Common Name(s) AI195024; AL033363; CGPx; GPXD; GPx-1; GSHPX1; GSHPx; GSHPx-1; Gpx; cellular GPx; expressed sequence AI195024; expressed sequence AL033363; glutathione peroxidase;
General Note Phenotypic Similarity to Human Syndrome: Inflammatory Bowel Disease (J:71506) as heterozygote or homozygote on a Gpx2tm2Coh homozygote background.
Molecular Note Insertion of a neomycin resistance cassette disrupted exon 2 of the gene. Northern blots of total RNA isolated from brain, heart, kidney, liver, and lung of homozygous mutant mice showed no detectable transcript of the targeted gene when probed with an a gene-specific cDNA probe. [MGI Ref ID J:41169]

Genotyping

Genotyping Information

Genotyping Protocols

Gpx1tm1Yshalternate1, Standard PCR
Park2tm1Shn, Melt Curve Analysis
Park7tm1Shn, High Resolution Melting


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Ho YS; Magnenat JL; Bronson RT; Cao J; Gargano M; Sugawara M ; Funk CD. 1997. Mice deficient in cellular glutathione peroxidase develop normally and show no increased sensitivity to hyperoxia. J Biol Chem 272(26):16644-51. [PubMed: 9195979]  [MGI Ref ID J:41169]

Additional References

Gpx1tm1Ysh related

Beck MA; Esworthy RS; Ho YS; Chu FF. 1998. Glutathione peroxidase protects mice from viral-induced myocarditis. FASEB J 12(12):1143-9. [PubMed: 9737717]  [MGI Ref ID J:82372]

Chan AW; Ho YS; Chung SK; Chung SS. 2008. Synergistic effect of osmotic and oxidative stress in slow-developing cataract formation. Exp Eye Res 87(5):454-61. [PubMed: 18760274]  [MGI Ref ID J:142446]

Cheng F; Torzewski M; Degreif A; Rossmann H; Canisius A; Lackner KJ. 2013. Impact of glutathione peroxidase-1 deficiency on macrophage foam cell formation and proliferation: implications for atherogenesis. PLoS One 8(8):e72063. [PubMed: 23991041]  [MGI Ref ID J:204544]

Cheng W; Fu YX; Porres JM; Ross DA; Lei XG. 1999. Selenium-dependent cellular glutathione peroxidase protects mice against a pro-oxidant-induced oxidation of NADPH, NADH, lipids, and protein. FASEB J 13(11):1467-75. [PubMed: 10428770]  [MGI Ref ID J:56648]

Cheng WH; Quimby FW; Lei XG. 2003. Impacts of glutathione peroxidase-1 knockout on the protection by injected selenium against the pro-oxidant-induced liver aponecrosis and signaling in selenium-deficient mice. Free Radic Biol Med 34(7):918-27. [PubMed: 12654481]  [MGI Ref ID J:118014]

Chu FF; Esworthy RS; Chu PG; Longmate JA; Huycke MM; Wilczynski S; Doroshow JH. 2004. Bacteria-Induced Intestinal Cancer in Mice with Disrupted Gpx1 and Gpx2 Genes. Cancer Res 64(3):962-968. [PubMed: 14871826]  [MGI Ref ID J:88070]

Dayal S; Brown KL; Weydert CJ; Oberley LW; Arning E; Bottiglieri T; Faraci FM; Lentz SR. 2002. Deficiency of glutathione peroxidase-1 sensitizes hyperhomocysteinemic mice to endothelial dysfunction. Arterioscler Thromb Vasc Biol 22(12):1996-2002. [PubMed: 12482825]  [MGI Ref ID J:103367]

Demchenko IT; Atochin DN; Gutsaeva DR; Godfrey RR; Huang PL; Piantadosi CA; Allen BW. 2008. Contributions of nitric oxide synthase isoforms to pulmonary oxygen toxicity, local vs. mediated effects. Am J Physiol Lung Cell Mol Physiol 294(5):L984-90. [PubMed: 18326824]  [MGI Ref ID J:136632]

Esworthy RS; Aranda R; Martin MG; Doroshow JH; Binder SW; Chu FF. 2001. Mice with combined disruption of Gpx1 and Gpx2 genes have colitis. Am J Physiol Gastrointest Liver Physiol 281(3):G848-55. [PubMed: 11518697]  [MGI Ref ID J:71506]

Esworthy RS; Binder SW; Doroshow JH; Chu FF. 2003. Microflora trigger colitis in mice deficient in selenium-dependent glutathione peroxidase and induce Gpx2 gene expression. Biol Chem 384(4):597-607. [PubMed: 12751789]  [MGI Ref ID J:103116]

Esworthy RS; Ho YS; Chu FF. 1997. The Gpx1 gene encodes mitochondrial glutathione peroxidase in the mouse liver. Arch Biochem Biophys 340(1):59-63. [PubMed: 9126277]  [MGI Ref ID J:39714]

Esworthy RS; Kim BW; Chow J; Shen B; Doroshow JH; Chu FF. 2014. Nox1 causes ileocolitis in mice deficient in glutathione peroxidase-1 and -2. Free Radic Biol Med 68:315-25. [PubMed: 24374371]  [MGI Ref ID J:211847]

Esworthy RS; Kim BW; Larson GP; Yip ML; Smith DD; Li M; Chu FF. 2011. Colitis locus on chromosome 2 impacting the severity of early-onset disease in mice deficient in GPX1 and GPX2. Inflamm Bowel Dis 17(6):1373-86. [PubMed: 20872835]  [MGI Ref ID J:171572]

Esworthy RS; Kim BW; Rivas GE; Leto TL; Doroshow JH; Chu FF. 2012. Analysis of candidate colitis genes in the Gdac1 locus of mice deficient in glutathione peroxidase-1 and -2. PLoS One 7(9):e44262. [PubMed: 22970191]  [MGI Ref ID J:191887]

Esworthy RS; Mann JR; Sam M; Chu FF. 2000. Low glutathione peroxidase activity in Gpx1 knockout mice protects jejunum crypts from gamma-irradiation damage. Am J Physiol Gastrointest Liver Physiol 279(2):G426-36. [PubMed: 10915653]  [MGI Ref ID J:63988]

Esworthy RS; Yang L; Frankel PH; Chu FF. 2005. Epithelium-specific glutathione peroxidase, gpx2, is involved in the prevention of intestinal inflammation in selenium-deficient mice. J Nutr 135(4):740-5. [PubMed: 15795427]  [MGI Ref ID J:97545]

Forgione MA; Cap A; Liao R; Moldovan NI; Eberhardt RT; Lim CC; Jones J; Goldschmidt-Clermont PJ; Loscalzo J. 2002. Heterozygous cellular glutathione peroxidase deficiency in the mouse: abnormalities in vascular and cardiac function and structure Circulation 106(9):1154-1158. [PubMed: 12196344]  [MGI Ref ID J:111478]

Forgione MA; Weiss N; Heydrick S; Cap A; Klings ES; Bierl C; Eberhardt RT; Farber HW; Loscalzo J. 2002. Cellular glutathione peroxidase deficiency and endothelial dysfunction. Am J Physiol Heart Circ Physiol 282(4):H1255-61. [PubMed: 11893559]  [MGI Ref ID J:75857]

Fu Y; Cheng WH; Porres JM; Ross DA; Lei XG. 1999. Knockout of cellular glutathione peroxidase gene renders mice susceptible to diquat-induced oxidative stress. Free Radic Biol Med 27(5-6):605-11. [PubMed: 10490281]  [MGI Ref ID J:59488]

Galasso G; Schiekofer S; Sato K; Shibata R; Handy DE; Ouchi N; Leopold JA; Loscalzo J; Walsh K. 2006. Impaired angiogenesis in glutathione peroxidase-1-deficient mice is associated with endothelial progenitor cell dysfunction. Circ Res 98(2):254-61. [PubMed: 16373599]  [MGI Ref ID J:118088]

Gao J; Xiong Y; Ho YS; Liu X; Chua CC; Xu X; Wang H; Hamdy R; Chua BH. 2008. Glutathione peroxidase 1-deficient mice are more susceptible to doxorubicin-induced cardiotoxicity. Biochim Biophys Acta 1783(10):2020-9. [PubMed: 18602426]  [MGI Ref ID J:140888]

Hahn MA; Hahn T; Lee DH; Esworthy RS; Kim BW; Riggs AD; Chu FF; Pfeifer GP. 2008. Methylation of polycomb target genes in intestinal cancer is mediated by inflammation. Cancer Res 68(24):10280-9. [PubMed: 19074896]  [MGI Ref ID J:142237]

Han C; Someya S. 2013. Mouse models of age-related mitochondrial neurosensory hearing loss. Mol Cell Neurosci 55:95-100. [PubMed: 22820179]  [MGI Ref ID J:203587]

Han ES; Muller FL; Perez VI; Qi W; Liang H; Xi L; Fu C; Doyle E; Hickey M; Cornell J; Epstein CJ; Roberts LJ; Van Remmen H; Richardson A. 2008. The in vivo gene expression signature of oxidative stress. Physiol Genomics 34(1):112-26. [PubMed: 18445702]  [MGI Ref ID J:145302]

Hennis MR; Marvin MA; Taylor CM 2nd; Goldberg MS. 2013. Surprising behavioral and neurochemical enhancements in mice with combined mutations linked to Parkinson's disease. Neurobiol Dis 62C:113-123. [PubMed: 24075852]  [MGI Ref ID J:202221]

Hill KE; Wu S; Motley AK; Stevenson TD; Winfrey VP; Capecchi MR; Atkins JF; Burk RF. 2012. Production of selenoprotein P (Sepp1) by hepatocytes is central to selenium homeostasis. J Biol Chem 287(48):40414-24. [PubMed: 23038251]  [MGI Ref ID J:192636]

Jiang D; Akopian G; Ho YS; Walsh JP; Andersen JK. 2000. Chronic brain oxidation in a glutathione peroxidase knockout mouse model results in increased resistance to induced epileptic seizures. Exp Neurol 164(2):257-68. [PubMed: 10915565]  [MGI Ref ID J:115327]

Johnson RM; Goyette G Jr; Ravindranath Y; Ho YS. 2005. Hemoglobin autoxidation and regulation of endogenous H(2)O(2) levels in erythrocytes. Free Radic Biol Med 39(11):1407-17. [PubMed: 16274876]  [MGI Ref ID J:102737]

Johnson RM; Goyette G; Ravindranath Y; Ho YS. 2000. Red cells from glutathione peroxidase-1-deficient mice have nearly normal defenses against exogenous peroxides Blood 96(5):1985-8. [PubMed: 10961904]  [MGI Ref ID J:64216]

Johnson RM; Ho YS; Yu DY; Kuypers FA; Ravindranath Y; Goyette GW. 2010. The effects of disruption of genes for peroxiredoxin-2, glutathione peroxidase-1, and catalase on erythrocyte oxidative metabolism. Free Radic Biol Med 48(4):519-525. [PubMed: 19969073]  [MGI Ref ID J:156771]

Keller JN; Huang FF; Zhu H; Yu J; Ho YS; Kindy TS. 2000. Oxidative stress-associated impairment of proteasome activity during ischemia-reperfusion injury. J Cereb Blood Flow Metab 20(10):1467-73. [PubMed: 11043909]  [MGI Ref ID J:133829]

Kim BW; Esworthy RS; Hahn MA; Pfeifer GP; Chu FF. 2012. Expression of lactoperoxidase in differentiated mouse colon epithelial cells. Free Radic Biol Med 52(9):1569-76. [PubMed: 22343415]  [MGI Ref ID J:183260]

Klivenyi P; Andreassen OA; Ferrante RJ; Dedeoglu A; Mueller G; Lancelot E; Bogdanov M; Andersen JK; Jiang D; Beal MF. 2000. Mice deficient in cellular glutathione peroxidase show increased vulnerability to malonate, 3-nitropropionic acid, and 1-methyl-4-phenyl-1,2,5,6-tetrahydropyridine. J Neurosci 20(1):1-7. [PubMed: 10627575]  [MGI Ref ID J:59142]

Lee DH; Esworthy RS; Chu C; Pfeifer GP; Chu FF. 2006. Mutation accumulation in the intestine and colon of mice deficient in two intracellular glutathione peroxidases. Cancer Res 66(20):9845-51. [PubMed: 17047045]  [MGI Ref ID J:114994]

Lee S; Shin HS; Shireman PK; Vasilaki A; Van Remmen H; Csete ME. 2006. Glutathione-peroxidase-1 null muscle progenitor cells are globally defective. Free Radic Biol Med 41(7):1174-84. [PubMed: 16962942]  [MGI Ref ID J:112583]

Lei XG; Zhu JH; McClung JP; Aregullin M; Roneker CA. 2006. Mice deficient in Cu,Zn-superoxide dismutase are resistant to acetaminophen toxicity. Biochem J 399(3):455-61. [PubMed: 16831125]  [MGI Ref ID J:116036]

Lim CC; Bryan NS; Jain M; Garcia-Saura MF; Fernandez BO; Sawyer DB; Handy DE; Loscalzo J; Feelisch M; Liao R. 2009. Glutathione peroxidase deficiency exacerbates ischemia-reperfusion injury in male but not female myocardium: insights into antioxidant compensatory mechanisms. Am J Physiol Heart Circ Physiol 297(6):H2144-53. [PubMed: 19801492]  [MGI Ref ID J:158230]

Liu G; Feinstein SI; Wang Y; Dodia C; Fisher D; Yu K; Ho YS; Fisher AB. 2010. Comparison of glutathione peroxidase 1 and peroxiredoxin 6 in protection against oxidative stress in the mouse lung. Free Radic Biol Med 49(7):1172-81. [PubMed: 20627125]  [MGI Ref ID J:164603]

Lubos E; Mahoney CE; Leopold JA; Zhang YY; Loscalzo J; Handy DE. 2010. Glutathione peroxidase-1 modulates lipopolysaccharide-induced adhesion molecule expression in endothelial cells by altering CD14 expression. FASEB J 24(7):2525-32. [PubMed: 20219985]  [MGI Ref ID J:162352]

Muller FL; Lustgarten MS; Jang Y; Richardson A; Van Remmen H. 2007. Trends in oxidative aging theories. Free Radic Biol Med 43(4):477-503. [PubMed: 17640558]  [MGI Ref ID J:123504]

Ohlemiller KK; McFadden SL; Ding DL; Lear PM; Ho YS. 2000. Targeted mutation of the gene for cellular glutathione peroxidase (Gpx1) increases noise-induced hearing loss in mice. J Assoc Res Otolaryngol 1(3):243-54. [PubMed: 11545230]  [MGI Ref ID J:113167]

Olson GE; Whitin JC; Hill KE; Winfrey VP; Motley AK; Austin LM; Deal J; Cohen HJ; Burk RF. 2009. EXTRACELLULAR GLUTATHIONE PEROXIDASE (Gpx3) BINDS SPECIFICALLY TO BASEMENT MEMBRANES OF MOUSE RENAL CORTEX TUBULE CELLS. Am J Physiol Renal Physiol :. [PubMed: 20015939]  [MGI Ref ID J:159745]

Rathore R; Zheng YM; Niu CF; Liu QH; Korde A; Ho YS; Wang YX. 2008. Hypoxia activates NADPH oxidase to increase [ROS]i and [Ca2+]i through the mitochondrial ROS-PKCepsilon signaling axis in pulmonary artery smooth muscle cells. Free Radic Biol Med 45(9):1223-31. [PubMed: 18638544]  [MGI Ref ID J:141206]

Reddy VN; Giblin FJ; Lin LR; Dang L; Unakar NJ; Musch DC; Boyle DL; Takemoto LJ; Ho YS; Knoernschild T; Juenemann A; Lutjen-Drecoll E. 2001. Glutathione peroxidase-1 deficiency leads to increased nuclear light scattering, membrane damage, and cataract formation in gene-knockout mice. Invest Ophthalmol Vis Sci 42(13):3247-55. [PubMed: 11726630]  [MGI Ref ID J:108431]

South PK; Levander OA; Smith AD. 2002. Effects of dietary iron overload on glutathione peroxidase knockout mice. Biol Trace Elem Res 88(1):79-85. [PubMed: 12117267]  [MGI Ref ID J:113070]

Spector A; Kuszak JR; Ma W; Wang RR. 2001. The effect of aging on glutathione peroxidase-i knockout mice-resistance of the lens to oxidative stress. Exp Eye Res 72(5):533-45. [PubMed: 11311045]  [MGI Ref ID J:69125]

Spector A; Kuszak JR; Ma W; Wang RR; Ho Y; Yang Y. 1998. The effect of photochemical stress upon the lenses of normal and glutathione peroxidase-1 knockout mice. Exp Eye Res 67(4):457-71. [PubMed: 9820794]  [MGI Ref ID J:106611]

Spector A; Yang Y; Ho YS; Magnenat JL; Wang RR; Ma W; Li WC. 1996. Variation in cellular glutathione peroxidase activity in lens epithelial cells, transgenics and knockouts does not significantly change the response to H2O2 stress. Exp Eye Res 62(5):521-40. [PubMed: 8759521]  [MGI Ref ID J:110762]

Torzewski M; Ochsenhirt V; Kleschyov AL; Oelze M; Daiber A; Li H; Rossmann H; Tsimikas S; Reifenberg K; Cheng F; Lehr HA; Blankenberg S; Forstermann U; Munzel T; Lackner KJ. 2007. Deficiency of glutathione peroxidase-1 accelerates the progression of atherosclerosis in apolipoprotein E-deficient mice. Arterioscler Thromb Vasc Biol 27(4):850-7. [PubMed: 17255533]  [MGI Ref ID J:135067]

Van Remmen H; Qi W; Sabia M; Freeman G; Estlack L; Yang H; Mao Guo Z; Huang TT; Strong R; Lee S; Epstein CJ; Richardson A. 2004. Multiple deficiencies in antioxidant enzymes in mice result in a compound increase in sensitivity to oxidative stress. Free Radic Biol Med 36(12):1625-34. [PubMed: 15182862]  [MGI Ref ID J:90710]

Vasilaki A; Csete M; Pye D; Lee S; Palomero J; McArdle F; Van Remmen H; Richardson A; McArdle A; Faulkner JA; Jackson MJ. 2006. Genetic modification of the manganese superoxide dismutase/glutathione peroxidase 1 pathway influences intracellular ROS generation in quiescent, but not contracting, skeletal muscle cells. Free Radic Biol Med 41(11):1719-25. [PubMed: 17145560]  [MGI Ref ID J:116698]

Walshe J; Serewko-Auret MM; Teakle N; Cameron S; Minto K; Smith L; Burcham PC; Russell T; Strutton G; Griffin A; Chu FF; Esworthy S; Reeve V; Saunders NA. 2007. Inactivation of glutathione peroxidase activity contributes to UV-induced squamous cell carcinoma formation. Cancer Res 67(10):4751-8. [PubMed: 17510403]  [MGI Ref ID J:121730]

Wang L; Jiang Z; Lei XG. 2012. Knockout of SOD1 alters murine hepatic glycolysis, gluconeogenesis, and lipogenesis. Free Radic Biol Med 53(9):1689-96. [PubMed: 22974764]  [MGI Ref ID J:190130]

Wolf N; Penn P; Pendergrass W; Van Remmen H; Bartke A; Rabinovitch P; Martin GM. 2005. Age-related cataract progression in five mouse models for anti-oxidant protection or hormonal influence. Exp Eye Res 81(3):276-85. [PubMed: 16129095]  [MGI Ref ID J:136934]

Yoshida T; Maulik N; Engelman RM; Ho YS; Magnenat JL; Rousou JA; Flack JE 3rd; Deaton D; Das DK. 1997. Glutathione peroxidase knockout mice are susceptible to myocardial ischemia reperfusion injury. Circulation 96(9 Suppl):II-216-20. [PubMed: 9386101]  [MGI Ref ID J:44499]

Zhu JH; Lei XG. 2006. Double null of selenium-glutathione peroxidase-1 and copper, zinc-superoxide dismutase enhances resistance of mouse primary hepatocytes to acetaminophen toxicity. Exp Biol Med (Maywood) 231(5):545-52. [PubMed: 16636302]  [MGI Ref ID J:135749]

Zhu JH; Lei XG. 2011. Lipopolysaccharide-induced hepatic oxidative injury is not potentiated by knockout of GPX1 and SOD1 in mice. Biochem Biophys Res Commun 404(1):559-63. [PubMed: 21145306]  [MGI Ref ID J:167441]

Zhu JH; Zhang X; McClung JP; Lei XG. 2006. Impact of Cu, Zn-superoxide dismutase and Se-dependent glutathione peroxidase-1 knockouts on acetaminophen-induced cell death and related signaling in murine liver. Exp Biol Med (Maywood) 231(11):1726-32. [PubMed: 17138759]  [MGI Ref ID J:135914]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX10

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, these mice can be bred as homozygotes.
Mating SystemHeterozygote x Heterozygote         (Female x Male)   06-MAR-14

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $232.00Female or MaleHeterozygous for Gpx1tm1Ysh  
$232.00Female or MaleHomozygous for Gpx1tm1Ysh  
Price per Pair (US dollars $)Pair Genotype
$464.00Heterozygous for Gpx1tm1Ysh x Heterozygous for Gpx1tm1Ysh  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $301.60Female or MaleHeterozygous for Gpx1tm1Ysh  
$301.60Female or MaleHomozygous for Gpx1tm1Ysh  
Price per Pair (US dollars $)Pair Genotype
$603.20Heterozygous for Gpx1tm1Ysh x Heterozygous for Gpx1tm1Ysh  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.


See Terms of Use tab for General Terms and Conditions


The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
Ordering Information
JAX® Mice
Surgical and Preconditioning Services
JAX® Services
Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form

Terms of Use

Terms of Use


General Terms and Conditions


Contact information

General inquiries regarding Terms of Use

Contracts Administration

phone:207-288-6470

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


(6.6)