Strain Name:

B6;129S-Pkmtm1.1Mgvh/J

Stock Number:

024048

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Availability:

Repository- Live

Use Restrictions Apply, see Terms of Use
These Pkm2fl/fl floxed mutant mice possess loxP sites flanking exon 10 of the Pkm gene. This strain may be useful for generating conditional mutations in applications related to the role of pyruvate kinase M2 isoform in tumor formation.

Description

Strain Information

Type Mutant Stock; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
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Mating SystemHeterozygote x Heterozygote         (Female x Male)   03-APR-14
Specieslaboratory mouse
Generation?+pN1 (20-JUN-14)
Generation Definitions
 
Donating Investigator Matthew G. Vander Heiden,  

Description
The Pkm, pyruvate kinase muscle, gene encodes different splicing variant isoenzymes which are involved in glycolysis. The pyruvate kinase M2 isoform is found in tumor cells and some normal tissues. These mice possess loxP sites on either side of exon 10 of the targeted gene. Mice that are homozygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 10 deleted in the cre-expressing tissues: pyruvate kinase M2 isoform expression is elimated, while pyruvate kinase M1 isoform expression is unchanged.
When bred to a cancer model, this mutant mouse strain may be useful in studies of acceleration of tumorigenesis and metastasis. It could also be used to test the role of PKM2 in normal tissue physiology.

Development
A targeting vector containing a FRT site flanked NEO cassette upstream of exon 9 of the targeted gene, and loxP sites flanking exon 10 was electroporated into 129S6/SvEvTac derived TC1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric animals were tested for germline transmission. The mice were crossed to mice (on an unconfirmed B6;129 genetic background) expressing FLP1 (FlpO) recombinase (ROSA26Flpo) to excise the NEO cassette. Mice that retained the loxP site flanked exon 10 were then bred to remove the FLP1 (FlpO) recombinase allele. Heterozygotes were crossed to generate homozygotes. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.

Control Information

  Control
   101043 B6129SF1/J (approximate)
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Pkm
000573   IS/CamRkJ
View Strains carrying other alleles of Pkm     (1 strain)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Pkmtm1.1Mgvh/Pkmtm1.1Mgvh

        involves: 129S4/SvJaeSor * 129S6/SvEvTac
  • normal phenotype
  • no abnormal phenotype detected
    • mice are healthy and have no overt phenotype   (MGI Ref ID J:205213)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Cancer Research
Cre-lox System
      loxP-flanked Sequences

Research Tools
Cre-lox System
      loxP-flanked Sequences

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Pkmtm1.1Mgvh
Allele Name targeted mutation 1.1, Matthew G Vander Heiden
Allele Type Targeted (Conditional ready (e.g. floxed), No functional change)
Common Name(s) Pkm2fl;
Strain of Origin129S6/SvEvTac
Gene Symbol and Name Pkm, pyruvate kinase, muscle
Chromosome 9
Gene Common Name(s) AA414905; AL024370; AL024424; CTHBP; HEL-S-30; OIP3; PK3; PKM12; PKM2; Pk-2; Pk-3; Pk3; Pkm2; TCB; THBP1; expressed sequence AA414905; expressed sequence AL024370; expressed sequence AL024424; pyruvate kinase; pyruvate kinase 3;
Molecular Note A targeting vector was designed to contain an FRT-flanked neomycin cassette upstream of exon 9, and loxP sites that flank exon 10. Mutant mice were bred with mice expressing FlpO recombinase (Gt(ROSA)26Sor) to remove the neomycin selection cassette. [MGI Ref ID J:205213]

Genotyping

Genotyping Information

Genotyping Protocols

Pkmtm1.1Mgvh, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Israelsen WJ; Dayton TL; Davidson SM; Fiske BP; Hosios AM; Bellinger G; Li J; Yu Y; Sasaki M; Horner JW; Burga LN; Xie J; Jurczak MJ; DePinho RA; Clish CB; Jacks T; Kibbey RG; Wulf GM; Di Vizio D; Mills GB; Cantley LC; Vander Heiden MG. 2013. PKM2 isoform-specific deletion reveals a differential requirement for pyruvate kinase in tumor cells. Cell 155(2):397-409. [PubMed: 24120138]  [MGI Ref ID J:205213]

Additional References

Pkmtm1.1Mgvh related

McKnight SL. 2014. Please keep me 2uned to PKM2. Mol Cell 53(5):683-4. [PubMed: 24606916]  [MGI Ref ID J:210630]

Health & husbandry

Health & Colony Maintenance Information

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, these mice can be bred as homozygotes.
Mating SystemHeterozygote x Heterozygote         (Female x Male)   03-APR-14
Diet Information New Diet as of April 7, 2014: Lab Diet® 5K0Q

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $232.00Female or MaleHeterozygous for Pkmtm1.1Mgvh  
$232.00Female or MaleHomozygous for Pkmtm1.1Mgvh  
Price per Pair (US dollars $)Pair Genotype
$464.00Heterozygous for Pkmtm1.1Mgvh x Heterozygous for Pkmtm1.1Mgvh  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $301.60Female or MaleHeterozygous for Pkmtm1.1Mgvh  
$301.60Female or MaleHomozygous for Pkmtm1.1Mgvh  
Price per Pair (US dollars $)Pair Genotype
$603.20Heterozygous for Pkmtm1.1Mgvh x Heterozygous for Pkmtm1.1Mgvh  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Control Information

  Control
   101043 B6129SF1/J (approximate)
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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