Strain Name:


Stock Number:


Order this mouse


Under Development - Now Accepting Orders

Estimated Available for Distribution Date: 11-AUG-14
Use Restrictions Apply, see Terms of Use
These Pkm2fl/fl floxed mutant mice possess loxP sites flanking exon 10 of the Pkm gene. This strain may be useful for generating conditional mutations in applications related to the role of pyruvate kinase M2 isoform in tumor formation.


Strain Information

Type Mutant Stock; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Mating SystemHeterozygote x Heterozygote         (Female x Male)   03-APR-14
Specieslaboratory mouse
Generation?+pN1 (20-JUN-14)
Generation Definitions
Donating Investigator Matthew G. Vander Heiden,  

The Pkm, pyruvate kinase muscle, gene encodes different splicing variant isoenzymes which are involved in glycolysis. The pyruvate kinase M2 isoform is found in tumor cells and some normal tissues. These mice possess loxP sites on either side of exon 10 of the targeted gene. Mice that are homozygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 10 deleted in the cre-expressing tissues: pyruvate kinase M2 isoform expression is elimated, while pyruvate kinase M1 isoform expression is unchanged.
When bred to a cancer model, this mutant mouse strain may be useful in studies of acceleration of tumorigenesis and metastasis. It could also be used to test the role of PKM2 in normal tissue physiology.

A targeting vector containing a FRT site flanked NEO cassette upstream of exon 9 of the targeted gene, and loxP sites flanking exon 10 was electroporated into 129S6/SvEvTac derived TC1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric animals were tested for germline transmission. The mice were crossed to mice (on an unconfirmed B6;129 genetic background) expressing FLP1 (FlpO) recombinase (ROSA26Flpo) to excise the NEO cassette. Mice that retained the loxP site flanked exon 10 were then bred to remove the FLP1 (FlpO) recombinase allele. Heterozygotes were crossed to generate homozygotes. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.

Control Information

   101043 B6129SF1/J (approximate)
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Pkm
000573   IS/CamRkJ
View Strains carrying other alleles of Pkm     (1 strain)


Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.


        involves: 129S4/SvJaeSor * 129S6/SvEvTac
  • normal phenotype
  • no abnormal phenotype detected
    • mice are healthy and have no overt phenotype   (MGI Ref ID J:205213)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Cancer Research
Cre-lox System
      loxP-flanked Sequences

Research Tools
Cre-lox System
      loxP-flanked Sequences

Genes & Alleles

Gene & Allele Information provided by MGI

Allele Symbol Pkmtm1.1Mgvh
Allele Name targeted mutation 1.1, Matthew G Vander Heiden
Allele Type Targeted (Conditional ready (e.g. floxed), No functional change)
Common Name(s) Pkm2fl;
Strain of Origin129S6/SvEvTac
Gene Symbol and Name Pkm, pyruvate kinase, muscle
Chromosome 9
Gene Common Name(s) AA414905; AL024370; AL024424; CTHBP; HEL-S-30; OIP3; PK3; PKM12; PKM2; Pk-2; Pk-3; Pk3; Pkm2; TCB; THBP1; expressed sequence AA414905; expressed sequence AL024370; expressed sequence AL024424; pyruvate kinase; pyruvate kinase 3;
Molecular Note A targeting vector was designed to contain an FRT-flanked neomycin cassette upstream of exon 9, and loxP sites that flank exon 10. Mutant mice were bred with mice expressing FlpO recombinase (Gt(ROSA)26Sor) to remove the neomycin selection cassette. [MGI Ref ID J:205213]


Genotyping Information

Genotyping Protocols

Pkmtm1.1Mgvh, Standard PCR

Helpful Links

Genotyping resources and troubleshooting


References provided by MGI

Selected Reference(s)

Israelsen WJ; Dayton TL; Davidson SM; Fiske BP; Hosios AM; Bellinger G; Li J; Yu Y; Sasaki M; Horner JW; Burga LN; Xie J; Jurczak MJ; DePinho RA; Clish CB; Jacks T; Kibbey RG; Wulf GM; Di Vizio D; Mills GB; Cantley LC; Vander Heiden MG. 2013. PKM2 isoform-specific deletion reveals a differential requirement for pyruvate kinase in tumor cells. Cell 155(2):397-409. [PubMed: 24120138]  [MGI Ref ID J:205213]

Additional References

Pkmtm1.1Mgvh related

McKnight SL. 2014. Please keep me 2uned to PKM2. Mol Cell 53(5):683-4. [PubMed: 24606916]  [MGI Ref ID J:210630]

Health & husbandry

Health & Colony Maintenance Information

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, these mice can be bred as homozygotes.
Mating SystemHeterozygote x Heterozygote         (Female x Male)   03-APR-14

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


This strain is currently Under Development - Now Accepting Orders.
Estimated Available for Distribution Date: 11-AUG-14

Please note: You may now place orders for this strain although it is not yet ready for distribution. Estimated available for distribution dates are provided to keep customers better informed on strains under development. Please note that our Colony Managers routinely monitor the target date and edit it based on breeding performance and other factors. The length of time it takes to make a new strain available for distribution depends on genotype, age, number of animals sent by the Donating Investigator, breeding performance, additional strain development (backcrossing, making homozygous), and anticipated demand for the strain.

Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $232.00Female or MaleHeterozygous for Pkmtm1.1Mgvh  
$232.00Female or MaleHomozygous for Pkmtm1.1Mgvh  
Price per Pair (US dollars $)Pair Genotype
$464.00Heterozygous for Pkmtm1.1Mgvh x Heterozygous for Pkmtm1.1Mgvh  

Standard Supply

Under Development - Now Accepting Orders The strain development process (i.e. importation, rederivation, and colony expansion) usually takes six to nine months.

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $301.60Female or MaleHeterozygous for Pkmtm1.1Mgvh  
$301.60Female or MaleHomozygous for Pkmtm1.1Mgvh  
Price per Pair (US dollars $)Pair Genotype
$603.20Heterozygous for Pkmtm1.1Mgvh x Heterozygous for Pkmtm1.1Mgvh  

Standard Supply

Under Development - Now Accepting Orders The strain development process (i.e. importation, rederivation, and colony expansion) usually takes six to nine months.

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Under Development - Now Accepting Orders The strain development process (i.e. importation, rederivation, and colony expansion) usually takes six to nine months.

Control Information

   101043 B6129SF1/J (approximate)
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

See Terms of Use tab for General Terms and Conditions

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
Ordering Information
JAX® Mice
Surgical and Preconditioning Services
JAX® Services
Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form

Terms of Use

Terms of Use

General Terms and Conditions

For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
- Use of MICE by companies or for-profit entities requires a license.

Contact information

General inquiries regarding Terms of Use

Contracts Administration


JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

No Warranty


In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.