Strain Name:

FVB-Tg(ACTA1*-TRPC3)1Jmol/J

Stock Number:

024052

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Availability:

Cryopreserved - Ready for recovery

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Mice overexpressing TRPC3 cDNA under the control of a modified human ACTA1 promoter show features of dystrophin-glycoprotein complex (DGC)-lacking dystrophic disease.

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Coisogenic; Mutant Strain; Transgenic;
Additional information on Genetically Engineered and Mutant Mice.
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Specieslaboratory mouse
 
Donating Investigator Jeffery D. Molkentin,   Cincinnati Children's Hospital

Description
TRPC (transient receptor potential canonical) genes have been implicated in the development of muscular dystrophy.

These transgenic animals overexpress human transient receptor potential canonical 3 (TRPC3) cDNA driven by a modified human ACTA1 (actin, alpha 1, skeletal muscle) promoter. A 6-fold increase in expression can be seen in skeletal muscle, but not other organs or tissues. Immunohistochemistry from the quadriceps confirms that the overexpressed protein is appropriately localized to the sarcolemma. An increase in calcium influx results in a phenotype similar to dystrophin-glycoprotein complex (DGC)-lacking dystrophic disease. Transgenic animals show profound wasting of all skeletal muscles examined at 2 months of age, as determined by direct measurement of muscle weights. Histological analysis of the muscles reveals increased central nucleation of fibers (due to degeneration/regeneration cycles), increased numbers of small myofibers, fibrosis, and infiltration of inflammatory cells. Disease progresses and by 6 months of age, fatty tissue replacement can be seen throughout muscle, along with increased fibrosis and greater variations in fiber area distributions with prominent macrophage infiltration. The heart is unaffected.

Development
This transgene expresses full-length human TRPC3 cDNA under the control of a modified human ACTA1 skeletal alpha-actin promoter (possibly a -2000 to +239 deletion construct). The transgenic vector was introduced to FVB embryos. Resultant mice were maintained on an FVB genetic background by the donating laboratory.

Control Information

  Control
   Noncarrier
   001800 FVB/NJ
 
  Considerations for Choosing Controls

Related Strains

View Strains carrying other alleles of ACTA1     (17 strains)

Strains carrying other alleles of TRPC3
016570   FVB-Tg(Myh6-TRPC3*)6.6Jmol/J
View Strains carrying other alleles of TRPC3     (1 strain)

Phenotype

Phenotype Information

View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Cell Biology Research
Channel and Transporter Defects
      calcium

Neurobiology Research
Muscular Dystrophy

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Tg(ACTA1-TRPC3)1Jmol
Allele Name transgene insertion 1, Jeffery Molkentin
Allele Type Transgenic (Inserted expressed sequence)
Common Name(s) TRPC3 TG;
Strain of OriginFVB
Expressed Gene TRPC3, transient receptor potential cation channel, subfamily C, member 3, human
Promoter ACTA1, actin, alpha 1, skeletal muscle, human
Molecular Note A modified human ACTA1 skeletal alpha-actin promoter (-2000 to +239) was used to drive muscle-specific expression of full-length human TRPC3 cDNA. Line 1 was generated. [MGI Ref ID J:154908]
 
 

Genotyping

Genotyping Information

Genotyping Protocols

Tg(ACTA1*-TRPC3)1Jmol, High Resolution Melting


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Millay DP; Goonasekera SA; Sargent MA; Maillet M; Aronow BJ; Molkentin JD. 2009. Calcium influx is sufficient to induce muscular dystrophy through a TRPC-dependent mechanism. Proc Natl Acad Sci U S A 106(45):19023-8. [PubMed: 19864620]  [MGI Ref ID J:154908]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryHemizygotes are viable and fertile. Homozygotes are viable, but the donating lab didn't use them in their breeding program.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2140.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We willfulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2782.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We willfulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   Noncarrier
   001800 FVB/NJ
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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