Strain Name:

B6.129P2-Senp1Gt(XG001)Byg/J

Stock Number:

024090

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Availability:

Cryopreserved - Ready for recovery

This Senp1 gene trap (knock out) strain is useful in studies of SUMOylation, regulation of protein-protein interactions and definitive erythropoiesis.

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Congenic; Gene Trap; Mutant Strain;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Specieslaboratory mouse
Generation+pN1
Generation Definitions
 
Donating Investigator Edward TH Yeh,   University of Texas M.D. Anderson Cancer Center

Description
SUMO1/sentrin specific peptidase 1 (SENP1) has de-SUMOylation function (deconjugates sumoylated proteins) and is involved in processes such as nuclear transport, posttranslational modification, transcriptional regulation, apoptosis, and protein stability. These mice carry a gene trap mutation of the Senp1 (SUMO/sentrin specific peptidase 1) gene, which results in expression of a beta-geo/SENP1 truncated protein that lacks the C terminal catalytic domain. Mice that are heterozygous for the targeted mutation are viable and fertile. Homozygotes are not viable, with most dying before E15. A truncated transcript is detected by RT-PCR and Western blot analysis of homozygous embryos. Homozygous embryos are severely anemic at E12.5 to E15.5, with decreased number of peripheral red blood cells (more than 75% fewer erythrocytes compared to wildtype) and fewer liver erythropoietic foci. There is an increase of apoptotic erythroid precursor cells in the fetal liver. During backcrossing, the Y chromosome may not have been fixed to the C57BL/6J genetic background.

Development
A gene trap vector (pGT1Lxf) containing the reporter beta-galactosidase/neomycin-resistance fusion gene (beta-geo) was inserted into intron 8. The construct was electroporated into unspecified 129P2/OlaHsd derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were tested for germline transmission. The mice were then backcrossed to C57BL/6 for more than 50 generations (see SNP note below). During backcrossing, the Y chromosome may not have been fixed to the C57BL/6J genetic background. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.

A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While one of the 27 markers in the genome was segregating, all 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a C57BL/6N genetic background.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Senp1Gt(XG001)Byg/Senp1Gt(XG001)Byg

        involves: 129P2/OlaHsd
  • mortality/aging
  • partial lethality throughout fetal growth and development
    • most mice die between E13 and E15   (MGI Ref ID J:141453)
  • hematopoietic system phenotype
  • *normal* hematopoietic system phenotype
    • despite a reduction in erythroid progenitors, mice exhibit normal numbers of granulocyte-macrophage and pluripotent hematopoietic stem cells   (MGI Ref ID J:141453)
    • abnormal erythropoiesis
      • mice exhibit fewer nucleated cells in the fetal livers and a decrease in the number or erythropoietic foci compared to in wild-type mice   (MGI Ref ID J:141453)
      • decreased erythrocyte cell number
        • at E15.5, mice exhibit 75% fewer erythrocytes compared to in wild-type or heterozygous mice   (MGI Ref ID J:141453)
      • decreased erythroid progenitor cell number
        • at E13.5, the number of Ter-119+ erythroid precursors in the fetal liver is decreased compared to in wild-type mice due to apoptosis   (MGI Ref ID J:141453)
        • at E13.5, fetal liver cells produce 40% fewer erythroid colony-forming unit compared to wild-type cells   (MGI Ref ID J:141453)
        • however, the number of erythroid burst-forming unit are normal and supplementing medium with erythropoietin prevents the apoptosis of erythrocyte progenitor cells   (MGI Ref ID J:141453)
    • anemia
      • at E15.5, mice exhibit severe fetal anemia   (MGI Ref ID J:141453)
  • growth/size/body phenotype
  • decreased fetal size
  • liver/biliary system phenotype
  • small liver
    • fetal liver size is smaller than normal   (MGI Ref ID J:141453)
  • integument phenotype
  • pallor
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Developmental Biology Research
Embryonic Lethality (Homozygous)

Hematological Research
Hematopoietic Defects

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Senp1Gt(XG001)Byg
Allele Name gene trap XG001, BayGenomics
Allele Type Gene trapped
Strain of Origin129P2/OlaHsd
Gene Symbol and Name Senp1, SUMO1/sentrin specific peptidase 1
Chromosome 15
Gene Common Name(s) 2310046A20Rik; D15Ertd528e; DNA segment, Chr 15, ERATO Doi 528, expressed; E330036L07Rik; RIKEN cDNA 2310046A20 gene; RIKEN cDNA E330036L07 gene; SuPr-2;
Molecular Note A gene trap vector pGT1Lxf, which contains a beta-geo cassette, was inserted into intron 8. The presence of a truncated transcript was confirmed by RT-PCR. [MGI Ref ID J:141453]

Genotyping

Genotyping Information

Genotyping Protocols

Senp1Gt(XG001)Byg, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Cheng J; Kang X; Zhang S; Yeh ET. 2007. SUMO-specific protease 1 is essential for stabilization of HIF1alpha during hypoxia. Cell 131(3):584-95. [PubMed: 17981124]  [MGI Ref ID J:141453]

Additional References

Senp1Gt(XG001)Byg related

Sharma P; Yamada S; Lualdi M; Dasso M; Kuehn MR. 2013. Senp1 is essential for desumoylating Sumo1-modified proteins but dispensable for Sumo2 and Sumo3 deconjugation in the mouse embryo. Cell Rep 3(5):1640-50. [PubMed: 23684609]  [MGI Ref ID J:198680]

Stryke D; Kawamoto M; Huang CC; Johns SJ; King LA; Harper CA; Meng EC; Lee RE; Yee A; L'Italien L; Chuang PT; Young SG; Skarnes WC; Babbitt PC; Ferrin TE. 2003. BayGenomics: a resource of insertional mutations in mouse embryonic stem cells. Nucleic Acids Res 31(1):278-81. [PubMed: 12520002]  [MGI Ref ID J:91812]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, these mice can be bred as heterozygotes. Homozygotes are not viable.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2140.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2782.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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