Strain Name:

STOCK Hcn4tm3Sev/J

Stock Number:

024284

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Availability:

Repository- Live

Use Restrictions Apply, see Terms of Use
HCN4nLacZ/H2BGFP mice are useful for visualizing cells in the first heart field of the developing cardiac crescent.

Description

Strain Information

Type Mutant Stock; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Mating SystemWild-type x Heterozygote         (Female x Male)   25-MAR-14
Mating SystemHeterozygote x Wild-type         (Female x Male)   25-MAR-14
Specieslaboratory mouse
 
Donating Investigator Sylvia Evans,   University of California, San Diego

Description
Heterozygous mice are viable and fertile, homozygotes are embryonic lethal. The HCN4nLacZ/H2BGFP allele expresses a floxed nuclear lacZ and a H2BGFP fusion protein from the hyperpolarization-activated, cyclic nucleotide-gated K+ 4 (HCN4) promoter/enhancer elements. The frt-flanked neo is still present in this strain. HCN4 is expressed in the first heart field of differentiating myocytes in the developing cardiac crescent. These mice express in the first heart field of the cardiac conduction system (CCS). When these mice are bred to mice that express Cre recombinase, resulting offspring will have the floxed-lacZ sequence removed, resulting in EGFP fluorescence in the CCS.

Development
A targeting vector was designed to insert a floxed nuclear lacZ followed by H2BGFP fusion protein, and frt-flanked neomycin resistance selection cassette, immediately before endogenous start codon of hyperpolarization-activated, cyclic nucleotide-gated K+ 4 (HCN4) gene. Specifically, the H2BGFP fusion protein consists of the coding sequence for the human HIST1H2BB gene [histone 1 H2bb] followed C-terminally by Enhanced Green Fluorescent Protein gene. This construct was electroporated into embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts and chimeric males were bred. These mice were maintained on a Black Swiss outbred background. Upon arrival at The Jackson Laboratory, mice were bred with C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.

Control Information

  Control
   Wild-type from the colony
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Hcn4
008780   B6.Cg-Hcn4tm1Rsei/J
024283   STOCK Hcn4tm2.1(cre/ERT2)Sev/J
View Strains carrying other alleles of Hcn4     (2 strains)

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested.
Brugada Syndrome 8; BRGDA8   (HCN4)
Sick Sinus Syndrome 2, Autosomal Dominant; SSS2   (HCN4)
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Hcn4tm3Sev/Hcn4tm3Sev

        involves: Black Swiss * C57BL/6J
  • mortality/aging
  • complete embryonic lethality   (MGI Ref ID J:101977)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Cardiovascular Research

Research Tools
lacZ Expression
Genetics Research
      Tissue/Cell Markers
      Tissue/Cell Markers: Cre-lox System

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Hcn4tm3Sev
Allele Name targeted mutation 3, Sylvia Evans
Allele Type Targeted (Null/Knockout, Reporter)
Common Name(s) HCN4H2BGFP; HCN4nlacZ;
Gene Symbol and Name Hcn4, hyperpolarization-activated, cyclic nucleotide-gated K+ 4
Chromosome 9
Gene Common Name(s) SSS2;
Molecular Note A targeting vector was designed to insert a floxed nuclear lacZ followed by H2BGFP fusion protein, and frt-flanked neomycin resistance selection cassette, immediately before endogenous start codon. The H2BGFP fusion protein consists of the coding sequence for the human HIST1H2BB gene [histone 1 H2bb] followed C-terminally by Enhanced Green Fluorescent Protein (EGFP) gene. [MGI Ref ID J:204126]

Genotyping

Genotyping Information

Genotyping Protocols

Fluorescent Proteins (Generic GFP),

Probe


Hcn4tm3,

MELT



Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Liang X; Wang G; Lin L; Lowe J; Zhang Q; Bu L; Chen Y; Chen J; Sun Y; Evans SM. 2013. HCN4 dynamically marks the first heart field and conduction system precursors. Circ Res 113(4):399-407. [PubMed: 23743334]  [MGI Ref ID J:204126]

Additional References

Hcn4tm3Sev related

The Jackson Laboratory. 2005. Information obtained from The Jackson Laboratory, Bar Harbor, ME Unpublished :.  [MGI Ref ID J:101977]

Health & husbandry

Health & Colony Maintenance Information

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, heterozygous mice may be bred to wildtype (noncarrier) mice from the colony. Homozygotes are embryonic lethal.
Mating SystemWild-type x Heterozygote         (Female x Male)   25-MAR-14
Heterozygote x Wild-type         (Female x Male)   25-MAR-14

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $232.00Female or MaleHeterozygous for Hcn4tm3.1Sev  
Price per Pair (US dollars $)Pair Genotype
$304.00Heterozygous for Hcn4tm3.1Sev x Wild-type for Hcn4tm3.1Sev  
$304.00Wild-type for Hcn4tm3.1Sev x Heterozygous for Hcn4tm3.1Sev  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $301.60Female or MaleHeterozygous for Hcn4tm3.1Sev  
Price per Pair (US dollars $)Pair Genotype
$395.20Heterozygous for Hcn4tm3.1Sev x Wild-type for Hcn4tm3.1Sev  
$395.20Wild-type for Hcn4tm3.1Sev x Heterozygous for Hcn4tm3.1Sev  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Control Information

  Control
   Wild-type from the colony
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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