Strain Name:

B6.129S6(Cg)-Wwc1tm1.1Rlh/J

Stock Number:

024415

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Availability:

Under Development - Now Accepting Orders

Estimated Available for Distribution Date: 30-MAR-15
Use Restrictions Apply, see Terms of Use
This strain carries a targeted knockout of the mouse Wwc1 (KIBRA) gene and is useful in studies of synaptic plasticity, learning, and memory.

Description

Strain Information

Former Names B6.129S6(Cg)-Wwc1tm1.2Rlh/J    (Changed: 06-MAY-14 )
Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
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Additional information on Congenic nomenclature.
Mating SystemHeterozygote x Heterozygote         (Female x Male)   20-NOV-14
Specieslaboratory mouse
 
Donating Investigator Richard L Huganir,   Johns Hopkins University; HHMI

Description
Mutations in the WWC1 (WW, C2 and coiled-coil domain containing 1; also called KIBRA) gene have been associated with human memory performance.

This strain carries a targeted knockout of the mouse Wwc1 gene. Western blot analysis confirmed the absence of protein expression in the brain. Adult homozygous mice demonstrate impaired long-term depression and long-term potentiation at hippocampal Schaffer collateral-CA1 synapses. They also show severe deficits in contextual fear learning and memory. WWC1/KIBRA directly binds PICK1 in vitro and in vivo and interacts with GluA1 (GRIA1), GluA2 (GRIA2), and several other synaptic proteins in an in vivo protein complex. Evidence suggests that the gene plays and important role in regulating AMPAR (α-amino-3-hydroxyl-5-methyl-4-isoxazole-propionate receptor) trafficking underlying synaptic plasticity and learning.

Development
A loxP site was introduced upstream of exon 4 and an FRT-flanked PGK-neomycin cassette followed by a loxP site was placed downstream of exon 5. The targeting vector was introduced to 129S6/SvEvTac-derived MC1 embryonic stem (ES) cells. Resultant germline mice were crossed with a CMV-Cre strain on a C57BL/6 background to excise the floxed region, resulting in an out-of-frame mutation. This strain was backcrossed to C57BL/6 for 10 generations by the donating laboratory.

Control Information

  Control
   Wild-type from the colony
 
  Considerations for Choosing Controls

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Wwc1tm1.1Rlh/Wwc1tm1.1Rlh

        involves: 129 * C57BL/6
  • behavior/neurological phenotype
  • impaired contextual conditioning behavior
    • during the training session and 24 hours after training   (MGI Ref ID J:178552)
    • however, mice exhibit normal conditioning immediately after training   (MGI Ref ID J:178552)
  • impaired cued conditioning behavior
    • during the training session and 24 hours after training   (MGI Ref ID J:178552)
    • however, mice exhibit normal conditioning immediately after training   (MGI Ref ID J:178552)
  • nervous system phenotype
  • reduced long term depression
    • long term depression induced by NMDA in adult is delayed compared to in wild-type mice   (MGI Ref ID J:178552)
    • however, juvenile mice exhibit normal synaptic plasticity   (MGI Ref ID J:178552)
  • reduced long term potentiation
    • long term potentiation induced with theta-burst stimulation in adult mice is delayed compared to in wild-type mice   (MGI Ref ID J:178552)
    • however, juvenile mice exhibit normal synaptic plasticity   (MGI Ref ID J:178552)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Neurobiology Research
Alzheimer's Disease
Behavioral and Learning Defects
      high anxiety

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Wwc1tm1.1Rlh
Allele Name targeted mutation 1.1, Richard L Huganir
Allele Type Targeted (Null/Knockout)
Gene Symbol and Name Wwc1, WW, C2 and coiled-coil domain containing 1
Chromosome 11
Gene Common Name(s) AA408228; AU017197; BC037006; HBEBP3; HBEBP36; KIBRA; MEMRYQTL; MGC:47054; PPP1R168; RGD1308329; cDNA sequence BC037006; expressed sequence AA408228; expressed sequence AU017197;
Molecular Note A loxP site was inserted upstream of exon 4. An FRT-flanked neo cassette with a 3' loxP site was inserted downstream of exon 5. Cre-mediated recombination removed the exon 4, exon 5, and the neo cassette. Western blot analysis confirmed the absence of protein expression in the brain. [MGI Ref ID J:178552]

Genotyping

Genotyping Information


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Additional References

Wwc1tm1.1Rlh related

Makuch L; Volk L; Anggono V; Johnson RC; Yu Y; Duning K; Kremerskothen J; Xia J; Takamiya K; Huganir RL. 2011. Regulation of AMPA receptor function by the human memory-associated gene KIBRA. Neuron 71(6):1022-9. [PubMed: 21943600]  [MGI Ref ID J:178552]

Health & husbandry

Health & Colony Maintenance Information

Colony Maintenance

Breeding & HusbandryHomozygotes and heterozygotes are viable and fertile.
Mating SystemHeterozygote x Heterozygote         (Female x Male)   20-NOV-14

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


 

This strain is currently Under Development - Now Accepting Orders.
Estimated Available for Distribution Date: 30-MAR-15

Please note: You may now place orders for this strain although it is not yet ready for distribution. Estimated available for distribution dates are provided to keep customers better informed on strains under development. Please note that our Colony Managers routinely monitor the target date and edit it based on breeding performance and other factors. The length of time it takes to make a new strain available for distribution depends on genotype, age, number of animals sent by the Donating Investigator, breeding performance, additional strain development (backcrossing, making homozygous), and anticipated demand for the strain.

Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $232.00Female or MaleHeterozygous for Wwc1tm1.1Rlh  
$232.00Female or MaleHomozygous for Wwc1tm1.1Rlh  
Price per Pair (US dollars $)Pair Genotype
$464.00Heterozygous for Wwc1tm1.1Rlh x Heterozygous for Wwc1tm1.1Rlh  

Standard Supply

Under Development - Now Accepting Orders The strain development process (i.e. importation, rederivation, and colony expansion) usually takes six to nine months.

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $301.60Female or MaleHeterozygous for Wwc1tm1.1Rlh  
$301.60Female or MaleHomozygous for Wwc1tm1.1Rlh  
Price per Pair (US dollars $)Pair Genotype
$603.20Heterozygous for Wwc1tm1.1Rlh x Heterozygous for Wwc1tm1.1Rlh  

Standard Supply

Under Development - Now Accepting Orders The strain development process (i.e. importation, rederivation, and colony expansion) usually takes six to nine months.

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Under Development - Now Accepting Orders The strain development process (i.e. importation, rederivation, and colony expansion) usually takes six to nine months.

Control Information

  Control
   Wild-type from the colony
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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