Strain Name:

B6.Cg-Tg(Sirt1)ASrn/J

Stock Number:

024510

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Availability:

Repository- Live

Use Restrictions Apply, see Terms of Use
Sirt1super mice have a threefold increase in SIRT1 expression directed by its endogenous promoter/enhancer regions on a BAC transgene. They may be useful when studying telomere deacetylation, DNA repair, energy homeostasis.

Description

Strain Information

Type Congenic; Mutant Strain; Transgenic;
Additional information on Genetically Engineered and Mutant Mice.
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Additional information on Congenic nomenclature.
Mating SystemNoncarrier x Hemizygote         (Female x Male)   05-FEB-14
Mating SystemHemizygote x Noncarrier         (Female x Male)   05-FEB-14
Specieslaboratory mouse
GenerationN5+pN1 (14-APR-14)
Generation Definitions
 
Donating Investigator Manuel Serrano,   INRIA Sophia Antipolis

Description
Mice homozygous for the Sirt1super BAC are viable and fertile. These mice express a wild-type mouse sirtuin 1 (Sirt1) gene directed by its endogenous promoter/enhancer regions on the BAC transgene. The donating investigator reports that genomic construct also contains the adjacent DnaJ (Hsp40) homolog, subfamily C, member 12 (Dnajc12) gene, but notes the absence of effect of DNAJC12 inhibition on the molecular pathways affected by SIRT1. SIRT1 is a protein deacetylase involved with telomere deacetylation, DNA repair, energy homeostasis. As such SIRT1 plays roles in telomere length maintenance, tumor growth, and improved metabolic syndrome caused by high fat diets. Sirt1super mice exhibit a threefold increase in SIRT1 expression in liver, brown adipose tissue, and muscle. On a high fat diet they show lower lipid induced inflammation, increased glucose tolerance, and are almost entirely protected from hepatic steatosis, although, they gain fat mass similar to wild-type mice. Sirt1super mice are protected from liver cancer promoted by high-fat diet, but are prone to thyroid and prostate cancer in combination with Pten-deficiency. Sirt1super mice have a normal longevity but some aging-associated phenotypes are improved.

Development
Bacterial artificial chromosome (BAC) library (CHORI) was used to obtain a 174 kb BAC (#RP23-119G23) containing the entire mouse Sirt1 (sirtuin 1) gene. This BAC was microinjected into fertilized (C57BL6 x CBA)F1 oocytes and founder mice, carrying one copy of the BAC, were bred to C57BL/6J.OlaHsd inbred mice for at least 5 generations. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.

Control Information

  Control
   Noncarrier
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Sirt1
026009   B6.129-Sirt1tm1Mcby/LguJ
008041   B6;129-Sirt1tm1Ygu/J
013080   C57BL/6-Actbtm3.1(Sirt1)Npa/J
008081   CBy.129(B6)-Sirt1tm1Ygu/J
View Strains carrying other alleles of Sirt1     (4 strains)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Tg(Sirt1)ASrn/?

        involves: C57BL/6 * CBA
  • behavior/neurological phenotype
  • increased food intake
    • increased food intake on high fat diet as compared to wild type control   (MGI Ref ID J:137868)
  • homeostasis/metabolism phenotype
  • improved glucose tolerance
    • improved glucose tolerance after 7 weeks on high fat diet as measured by area-under-the-curve values as compared to controls   (MGI Ref ID J:137868)
    • blood glucose levels in transgenic mice fed high fat diet decrease more rapidly than controls following administration of IP glucose   (MGI Ref ID J:137868)
    • blood glucose levels in transgenic mice fed high fat diet do not increase as much as controls following pyruvate tolerance test (PTT)   (MGI Ref ID J:137868)
  • increased energy expenditure
    • increased energy expenditure on high fat diet as compared to wild type control   (MGI Ref ID J:137868)
  • immune system phenotype
  • increased susceptibility to endotoxin shock   (MGI Ref ID J:137868)
  • liver/biliary system phenotype
  • decreased susceptibility to hepatic steatosis
    • reduced susceptibility to hepatosteatosis after 19 weeks on high fat diet   (MGI Ref ID J:137868)
    • transgenic mice present fewer lipid droplets in the liver than controls following 19 weeks on high fat diet   (MGI Ref ID J:137868)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Cancer Research
Increased Tumor Incidence
      Prostate Tumors
Other
      DNA Repair

Cell Biology Research
DNA Damage Response

Developmental Biology Research
Internal/Organ Defects
      liver
      prostate

Diabetes and Obesity Research

Endocrine Deficiency Research
Pancreas Defects
Thyroid Defects

Internal/Organ Research
Liver Defects
Prostate
Thyroid Defects

Metabolism Research
Lipid Metabolism

Research Tools
Metabolism Research

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Tg(Sirt1)ASrn
Allele Name transgene insertion A, Manuel Serrano
Allele Type Transgenic (Inserted expressed sequence)
Common Name(s) SIRT1super; Sirt1-tg;
Strain of Origin(C57BL/6 x CBA)F1
Expressed Gene Sirt1, sirtuin 1, mouse, laboratory
Promoter Sirt1, sirtuin 1, mouse, laboratory
Molecular Note Bacterial artificial chromosome (BAC) library (CHORI) was used to obtain a 174 kb BAC (#RP23-119G23) containing the entire mouse Sirt1 gene and the adjacent Dnajc12 gene. Overexpression of Dnajc12 is not expected to affect Sirt1 molecular pathways. The one founder mouse created exhibits a 2-4 fold overexpression of SIRT1 protein in liver, kidney, thymus, spleen, colon and jejunum/ileum. [MGI Ref ID J:137868]
 

Genotyping

Genotyping Information

Genotyping Protocols

Tg(Sirt1)ASrn,

MELT



Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Pfluger PT; Herranz D; Velasco-Miguel S; Serrano M; Tschop MH. 2008. Sirt1 protects against high-fat diet-induced metabolic damage. Proc Natl Acad Sci U S A 105(28):9793-8. [PubMed: 18599449]  [MGI Ref ID J:137868]

Additional References

Tg(Sirt1)ASrn related

Palacios JA; Herranz D; De Bonis ML; Velasco S; Serrano M; Blasco MA. 2010. SIRT1 contributes to telomere maintenance and augments global homologous recombination. J Cell Biol 191(7):1299-313. [PubMed: 21187328]  [MGI Ref ID J:167988]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX11

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony homozygous mice may be bred together.
Mating SystemNoncarrier x Hemizygote         (Female x Male)   05-FEB-14
Hemizygote x Noncarrier         (Female x Male)   05-FEB-14

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $232.00Female or MaleHemizygous for Tg(Sirt1)ASrn  
Price per Pair (US dollars $)Pair Genotype
$304.00Hemizygous for Tg(Sirt1)ASrn x Noncarrier  
$304.00Noncarrier x Hemizygous for Tg(Sirt1)ASrn  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $301.60Female or MaleHemizygous for Tg(Sirt1)ASrn  
Price per Pair (US dollars $)Pair Genotype
$395.20Hemizygous for Tg(Sirt1)ASrn x Noncarrier  
$395.20Noncarrier x Hemizygous for Tg(Sirt1)ASrn  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Control Information

  Control
   Noncarrier
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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