Strain Name:

C3A.Cg-Pde6b+ Bmp4tm1Blh/SjJ

Stock Number:

024700

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Estimated Available for Distribution Date: 16-MAR-15
Bmp4tm1Blh homozygous targeted mutation mice show developmental defects including polydactyly, craniofacial defects, and cystic kidneys. Heterozygotes have also been reported to show eye abnormalities.

Description

Strain Information

Type Wild-Type Allele;
Type Congenic; Mutant Strain; Targeted Mutation;
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Additional information on Congenic nomenclature.
Mating SystemWild-type x Heterozygote         (Female x Male)   15-MAY-14
Mating SystemHeterozygote x Wild-type         (Female x Male)   15-MAY-14
Specieslaboratory mouse
 
Donating InvestigatorDr. Simon John,   The Jackson Laboratory

Description
Mice homozygous for the Bmp4tm1Blh targeted mutation mice die early in embryogenesis. Fifteen to twenty percent of heterozygous mice show developmental defects including polydactyly, craniofacial defects, and cystic kidneys. Heterozygotes have also been reported to show eye abnormalities.

This strain is on a genetic background different from that on which the allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.

Development
This strain was developed in the laboratory of Dr. Brigid Hogan at Vanderbilt Medical School. Coding sequences located after the seventh codon of exon 1 were replaced with a neomycin cassette via homologous recombination in 129S2/SvPas-derived D3 ES cells. The mutation was backcrossed to the C57BL/6 background for 11 generations (N11), then backcrossed to C3.BliA Pde6b+ (see Stock No. 001912) for 40 generations by the donating laboratory.

Control Information

  Control
   Wild-type from the colony
 
  Considerations for Choosing Controls

Related Strains

Strains carrying   Bmp4tm1Blh allele
002612   B6.129S2-Bmp4tm1Blh/J
View Strains carrying   Bmp4tm1Blh     (1 strain)

View Strains carrying   Pde6b+     (10 strains)

Strains carrying other alleles of Bmp4
016878   B6;129S4-Bmp4tm1Jfm/J
View Strains carrying other alleles of Bmp4     (1 strain)

Strains carrying other alleles of Pde6b
004202   B6.C3 Pde6brd1 Hps4le/+ +-Lmx1adr-8J/J
000002   B6.C3-Pde6brd1 Hps4le/J
004297   B6.CXB1-Pde6brd10/J
001022   B6C3FeF1/J a/a
000652   BDP/J
000653   BUB/BnJ
002439   C3.129P2(B6)-B2mtm1Unc/J
005494   C3.129S1(B6)-Grm1rcw/J
000509   C3.Cg-Lystbg-2J/J
000480   C3.MRL-Faslpr/J
001957   C3A Pde6brd1.O20/A-Prph2Rd2/J
004326   C3Bir.129P2(B6)-Il10tm1Cgn/Lt
003968   C3Bir.129P2(B6)-Il10tm1Cgn/LtJ
006435   C3Fe.SW-Soaa/MonJ
001904   C3H-Atcayji-hes/J
000659   C3H/HeJ
000511   C3H/HeJ-Ap3d1mh-2J/J
000784   C3H/HeJ-Faslgld/J
002433   C3H/HeJ-Sptbn4qv-lnd2J/J
005972   C3H/HeJBirLtJ
001824   C3H/HeJSxJ
000635   C3H/HeOuJ
000474   C3H/HeSn
001431   C3H/HeSn-ocd/J
000661   C3H/HeSnJ
002333   C3H/HeSnJ-gri/J
001576   C3He-Atp7btx-J/J
000658   C3HeB/FeJ
002588   C3HeB/FeJ-Eya1bor/J
001533   C3HeB/FeJ-Mc1rE-so Gli3Xt-J/J
001908   C3HfB/BiJ
001502   C3Sn.B6-Epha4rb/EiGrsrJ
002235   C3Sn.C3-Ctnna2cdf/J
001547   C3Sn.Cg-Cm/J
001906   C3fBAnl.Cg-Catb/AnlJ
004766   C57BL/6J-Pde6brd1-2J/J
000656   CBA/J
000813   CBA/J-Atp7aMo-pew/J
000660   DA/HuSnJ
000023   FL/1ReJ
000025   FL/4ReJ
003024   FVB.129P2(B6)-Fmr1tm1Cgr/J
002539   FVB.129P2-Abcb4tm1Bor/J
002935   FVB.129S2(B6)-Ccnd1tm1Wbg/J
002953   FVB.Cg-Tg(MMTVTGFA)254Rjc/J
003170   FVB.Cg-Tg(Myh6-tTA)6Smbf/J
003078   FVB.Cg-Tg(WapIgf1)39Dlr/J
003487   FVB.Cg-Tg(XGFAP-lacZ)3Mes/J
003257   FVB/N-Tg(GFAPGFP)14Mes/J
002856   FVB/N-Tg(TIE2-lacZ)182Sato/J
002384   FVB/N-Tg(UcpDta)1Kz/J
001800   FVB/NJ
001491   FVB/NMob
000804   HPG/BmJ
000734   MOLD/RkJ
000550   MOLF/EiJ
002423   NON/ShiLtJ
000679   P/J
000680   PL/J
000268   RSV/LeJ
000269   SB/LeJ
010968   SB;C3Sn-Lrp4mdig-2J/GrsrJ
005651   SJL.AK-Thy1a/TseJ
000686   SJL/J
000688   ST/bJ
004808   STOCK Mapttm1(EGFP)Klt Tg(MAPT)8cPdav/J
002648   STOCK a/a Cln6nclf/J
000279   STOCK gr +/+ Ap3d1mh/J
005965   STOCK Tg(Pomc1-cre)16Lowl/J
004770   SW.B6-Soab/J
002023   SWR.M-Emv21 Emv22/J
000689   SWR/J
000939   SWR/J-Clcn1adr-mto/J
000692   WB/ReJ KitW/J
100410   WBB6F1/J-KitW/KitW-v/J
000693   WC/ReJ KitlSl/J
View Strains carrying other alleles of Pde6b     (76 strains)

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Model with phenotypic similarity to human disease where etiologies are distinct. Human genes are associated with this disease. Orthologs of these genes do not appear in the mouse genotype(s).
Axenfeld-Rieger Syndrome, Type 3; RIEG3
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested.
Microphthalmia, Syndromic 6; MCOPS6   (BMP4)
Orofacial Cleft 11; OFC11   (BMP4)
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Bmp4tm1Blh/Bmp4+

        involves: 129S2/SvPas * C57BL/6 * CBA
  • reproductive system phenotype
  • decreased primordial germ cell number
    • fewer PGCs due to a reduced founder population rather than impaired expansion   (MGI Ref ID J:53311)
    • estimated 62% reduction in PGC founder population of mice on the C57BL/6, CBA mixed background   (MGI Ref ID J:53311)
    • PGCs were absent in 9% of mutant mice on the C57BL/6, CBA mixed background   (MGI Ref ID J:53311)
    • fewer Dppa3+ primordial germ cells than in wild-type mice   (MGI Ref ID J:199855)
  • behavior/neurological phenotype
  • circling
    • in 22 of 60 of mice with bilateral lateral semicircular canal defects   (MGI Ref ID J:159227)
  • hearing/vestibular/ear phenotype
  • abnormal lateral semicircular canal morphology
    • only 9% of mice exhibit abnormal semicircular canal on a mixed 129S2/SvPas, C57BL/6, and CBA background compared to 70% of females and 62% of males on a mixed 129S2/SvPas and C57BL/6 background   (MGI Ref ID J:159227)

Bmp4tm1Blh/Bmp4+

        B6.129S2-Bmp4tm1Blh/J
  • mortality/aging
  • partial perinatal lethality
    • less than half as many heterozygotes are born as expected   (MGI Ref ID J:42445)
  • vision/eye phenotype
  • abnormal eye electrophysiology
    • in some eyes, both a- and b-wave amplitude are reduced, due in part to poor pupillary dilation, with preferential loss of b-wave relative to a-wave   (MGI Ref ID J:82877)
  • abnormal eye morphology
    • mutants on a C57BL/6J background exhibit variable anterior and posterior segment abnormalities that are reduced on a mixed C3Hf/HeA and C57BL/LiA background and are rarely seen in mutants on CAST/Ei, 129S6/SvEvTac, or BALB/cJ background   (MGI Ref ID J:82877)
    • abnormal anterior eye segment morphology
      • variable anterior segment abnormalities in mutants 3-5 months of age   (MGI Ref ID J:82877)
      • abnormal cornea morphology
        • extracellular matrix (ECM) abnormalities are seen in the peripheral cornea, showing irregularly arranged collagen bundles   (MGI Ref ID J:82877)
        • anterior iris synechia
          • abnormal iridocorneal attachments (anterior synechiae) of variable extent   (MGI Ref ID J:82877)
        • corneal opacity
          • some eyes exhibit scleralization (opacity) of the the peripheral cornea or diffuse corneal haze   (MGI Ref ID J:82877)
        • decreased cornea thickness
          • peripheral cornea is often thinner with neovascularization   (MGI Ref ID J:82877)
      • abnormal iridocorneal angle
        • abnormal in most eyes   (MGI Ref ID J:82877)
        • abnormal canal of Schlemm morphology
          • small or absent   (MGI Ref ID J:82877)
          • absent Schlemm's canal
            • small or absent   (MGI Ref ID J:82877)
        • abnormal line of Schwalbe morphology
          • displaced Schwalbe's line   (MGI Ref ID J:82877)
        • absent trabecular meshwork
          • hypoplastic or absent trabecular meshwork that appears compressed and stalled in development   (MGI Ref ID J:82877)
        • hypoplastic trabecular meshwork
          • hypoplastic or absent trabecular meshwork that appears compressed and stalled in development   (MGI Ref ID J:82877)
      • abnormal placement of pupils
        • pupils are often eccentrically located   (MGI Ref ID J:82877)
      • cataracts   (MGI Ref ID J:82877)
        • anterior cortical cataracts
          • anterior subcapsular and cortical contaracts occur in most mutants   (MGI Ref ID J:82877)
        • anterior subcapsular cataracts
          • anterior subcapsular and cortical contaracts occur in most mutants   (MGI Ref ID J:82877)
      • iris hypoplasia
        • iris is generally normal, however in some eyes, the iris is hypoplastic and malformed; occasionally the malformation is extensive involving both iris and ciliary body   (MGI Ref ID J:82877)
      • irregularly shaped pupil   (MGI Ref ID J:82877)
    • abnormal eye size   (MGI Ref ID J:42445)
      • microphthalmia
        • frequency increased 3 fold   (MGI Ref ID J:42445)
    • abnormal posterior eye segment morphology
      • variable posterior eye segment abnormalities   (MGI Ref ID J:82877)
      • abnormal optic nerve morphology
        • abnormalities of the optic nerve head are frequently observed   (MGI Ref ID J:82877)
        • optic nerve abnormalities range from normal to absent, and are most often severely abnormal consisting of loose connective tissue, with absence of neural tissue   (MGI Ref ID J:82877)
        • absent optic nerve
          • the optic nerve is sometimes absent   (MGI Ref ID J:82877)
      • abnormal retina morphology   (MGI Ref ID J:82877)
        • abnormal retinal blood vessel pattern
          • the main retinal vessels branch close to the optic nerve and are irregularly arranged compared to wild-type   (MGI Ref ID J:82877)
        • abnormal retinal photoreceptor layer morphology
          • photoreceptor layer typically appears normal, however there are foci of retinal dysplasia, characterized by rosette formation   (MGI Ref ID J:82877)
        • retinal detachment
          • about 25% of heterozygotes exhibit retinal detachment as early as P30, with increased incidence as mice age   (MGI Ref ID J:82877)
        • thin retinal ganglion layer
          • retinal ganglion cell layer on average contains about 50% the normal number of cells   (MGI Ref ID J:82877)
        • thin retinal inner nuclear layer   (MGI Ref ID J:82877)
      • abnormal vitreous body morphology
        • dense network of small tortuous vessels throughout the vitreous that leak fluorescein, indicating compromised integrity of the vessels   (MGI Ref ID J:82877)
        • opacity of vitreous body
          • irregular white patches in the vitreous and dense vitreous haze in the majority of eyes   (MGI Ref ID J:82877)
        • persistence of hyaloid vascular system
          • abnormal persistence of the anterior hyaloid vessels   (MGI Ref ID J:82877)
          • posterior hyaloid vessels persist throughout the 17 month period studied and increase in number and size beyond that normally seen at birth   (MGI Ref ID J:82877)
    • anophthalmia
      • frequency increased 3 fold   (MGI Ref ID J:42445)
  • ocular hypertension
    • mutants with severe drainage structure abnormalities over 80% or more of their angle's extent have elevated intraocular pressure   (MGI Ref ID J:82877)
  • hearing/vestibular/ear phenotype
  • abnormal organ of Corti morphology
    • 2 of 4 circler and 2 of 4 non-circler heterozygotes show reduced neuronal processes in the organ of Corti   (MGI Ref ID J:118380)
    • in contrast, the saccule, utricle and ampullae of heterozygotes show normal numbers of neuronal processes   (MGI Ref ID J:118380)
  • abnormal vestibulocollic reflex
    • circling heterozygotes display low gain ratios with yaw axis rotation in the vestibulo-collic reflex, indicating poorer head stability relative to wild-type mice; poor response is consistent with horizontal semicircular canal dysfunction   (MGI Ref ID J:118380)
    • in the pitch axis, circling heterozygotes display as good or better head stability than wild-type mice, with gain ratios being greater or equal to 1   (MGI Ref ID J:118380)
    • in contrast, non-circling heterozygotes have gain ratios of greater or equal to 1 in both the yaw and pitch axes, indicating normal VCR function   (MGI Ref ID J:118380)
  • decreased vestibular hair cell stereocilia number
    • 4 of 6 circling heterozygotes display a significantly reduced number of stereocilia in their ampullae relative to wild-type; the other two circlers show a patchy decrease in the number of stereocilia   (MGI Ref ID J:118380)
    • in contrast, circling heterozygotes show normal stereocilia in the organ of Corti, saccule and utricle   (MGI Ref ID J:118380)
    • non-circling heterozygotes have normal-appearing stereocilia in both auditory and vestibular tissues   (MGI Ref ID J:118380)
  • impaired hearing
    • both circling and non-circling heterozygotes display a partial hearing loss, as assessed by ABR testing   (MGI Ref ID J:118380)
  • increased or absent threshold for auditory brainstem response
    • most circling heterozygotes exhibit elevated ABR thresholds across all test frequencies (7 out of 11 mice at 6 kHz and 12 kHz; 8 out of 11 mice at 24 kHz)   (MGI Ref ID J:118380)
    • non-circlers display elevated ABR thresholds similar to those of circlers   (MGI Ref ID J:118380)
  • nervous system phenotype
  • abnormal optic nerve morphology
    • abnormalities of the optic nerve head are frequently observed   (MGI Ref ID J:82877)
    • optic nerve abnormalities range from normal to absent, and are most often severely abnormal consisting of loose connective tissue, with absence of neural tissue   (MGI Ref ID J:82877)
    • absent optic nerve
      • the optic nerve is sometimes absent   (MGI Ref ID J:82877)
  • abnormal sensory neuron innervation pattern
    • neuronal processes innervating the cochlea of both circling and non-circling heterozygotes mice are reduced in number   (MGI Ref ID J:118380)
  • decreased vestibular hair cell stereocilia number
    • 4 of 6 circling heterozygotes display a significantly reduced number of stereocilia in their ampullae relative to wild-type; the other two circlers show a patchy decrease in the number of stereocilia   (MGI Ref ID J:118380)
    • in contrast, circling heterozygotes show normal stereocilia in the organ of Corti, saccule and utricle   (MGI Ref ID J:118380)
    • non-circling heterozygotes have normal-appearing stereocilia in both auditory and vestibular tissues   (MGI Ref ID J:118380)
  • behavior/neurological phenotype
  • abnormal vestibulocollic reflex
    • circling heterozygotes display low gain ratios with yaw axis rotation in the vestibulo-collic reflex, indicating poorer head stability relative to wild-type mice; poor response is consistent with horizontal semicircular canal dysfunction   (MGI Ref ID J:118380)
    • in the pitch axis, circling heterozygotes display as good or better head stability than wild-type mice, with gain ratios being greater or equal to 1   (MGI Ref ID J:118380)
    • in contrast, non-circling heterozygotes have gain ratios of greater or equal to 1 in both the yaw and pitch axes, indicating normal VCR function   (MGI Ref ID J:118380)
  • circling
    • by 2 weeks of age, 10% of heterozygous pups display circling behavior   (MGI Ref ID J:118380)
    • 1 of 2 circlers spent 50% of the time circling in a clockwise direction, 17% in a counterclockwise direction and 33% not circling   (MGI Ref ID J:118380)
    • the second circler spent 65% of the time circling in a clockwise direction, 1% in a counterclockwise direction and 34% not circling   (MGI Ref ID J:118380)
  • reproductive system phenotype
  • abnormal fertility/fecundity   (MGI Ref ID J:118380)
    • decreased litter size
      • average litter from mating a wild-type C57BL/6 mouse with a C57BL/6 heterozygote yields only 1 heterozygous pup   (MGI Ref ID J:118380)
    • reduced female fertility
      • female heterozygotes are poorer breeders relative to wild-type females   (MGI Ref ID J:118380)
  • abnormal male reproductive system morphology   (MGI Ref ID J:42445)
    • abnormal prostate gland anterior lobe morphology
      • sometimes cystic   (MGI Ref ID J:42445)
      • sometimes with abnormal lobulation   (MGI Ref ID J:42445)
    • abnormal seminiferous tubule morphology
      • sometimes cystic   (MGI Ref ID J:42445)
  • cardiovascular system phenotype
  • abnormal retinal blood vessel pattern
    • the main retinal vessels branch close to the optic nerve and are irregularly arranged compared to wild-type   (MGI Ref ID J:82877)
  • renal/urinary system phenotype
  • hydronephrosis
    • marked hydronephrosis in 12% of heterozygotes but ureter is undilated   (MGI Ref ID J:42445)
  • kidney atrophy
    • cortex of remaining kidney is atrophic   (MGI Ref ID J:42445)
  • kidney cysts
    • single cystic kidney in about 12% of heterozygotes   (MGI Ref ID J:42445)
    • multiple cysts involving both tubules and glomeruli   (MGI Ref ID J:42445)
  • single kidney
    • single cystic kidney in about 12% of heterozygotes   (MGI Ref ID J:42445)
  • craniofacial phenotype
  • short frontal bone
    • in about 12% of individuals   (MGI Ref ID J:42445)
  • short nasal bone
    • in about 12% of individuals   (MGI Ref ID J:42445)
  • limbs/digits/tail phenotype
  • polydactyly
    • 12% with unilateral anterior polydactyly involving the right hind limb only   (MGI Ref ID J:42445)
  • skeleton phenotype
  • short frontal bone
    • in about 12% of individuals   (MGI Ref ID J:42445)
  • short nasal bone
    • in about 12% of individuals   (MGI Ref ID J:42445)
  • endocrine/exocrine gland phenotype
  • abnormal prostate gland anterior lobe morphology
    • sometimes cystic   (MGI Ref ID J:42445)
    • sometimes with abnormal lobulation   (MGI Ref ID J:42445)
  • abnormal seminiferous tubule morphology
    • sometimes cystic   (MGI Ref ID J:42445)

Bmp4tm1Blh/Bmp4+

        involves: 129S2/SvPas * C3Hf/HeA * C57BL/LiA
  • vision/eye phenotype
  • abnormal anterior eye segment morphology
    • on a C3Hf/HeA and C57BL/LiA background, fewer mutants exhibit anterior segment abnormalities, with only half showing defects compared to 2/3 of mutants on a C57BL/6J background   (MGI Ref ID J:82877)
    • abnormal iridocorneal angle   (MGI Ref ID J:82877)
    • mydriasis
      • 5 of 13 mutants exhibit enlarged pupils   (MGI Ref ID J:82877)
  • buphthalmos   (MGI Ref ID J:82877)

Bmp4tm1Blh/Bmp4+

        involves: 129S2/SvPas * CAST/Ei
  • vision/eye phenotype
  • cataracts
    • on a CAST/Ei background, only one of seven mice develops a cataract compared to multiple and variable eye abnormalities in mice on a C57BL/6J background   (MGI Ref ID J:82877)

Bmp4tm1Blh/Bmp4+

        involves: 129S2/SvPas * 129S6/SvEvTac
  • vision/eye phenotype
  • abnormal eye morphology
    • incidence of eye abnormalities is much lower on the 129S6/SvEvTac background than on a C57BL/6J background, with only 1 of 12 mice showing persistent vitreous vessels and abnormal pupil/iris   (MGI Ref ID J:82877)
    • abnormal iris morphology
      • 1 of 12 mice shows persistent vitreous vessels   (MGI Ref ID J:82877)
    • persistence of hyaloid vascular system
      • 1 of 12 mice shows abnormal pupil/iris   (MGI Ref ID J:82877)
  • reproductive system phenotype
  • decreased primordial germ cell number
    • fewer primordial germ cells due to a reduced founder population rather than impaired expansion   (MGI Ref ID J:53311)
    • estimated 55% reduction in PGC founder population of mice on the 129S/SvEv, Black Swiss mixed background   (MGI Ref ID J:53311)

Bmp4tm1Blh/Bmp4+

        involves: 129S2/SvPas * BALB/cJ
  • vision/eye phenotype
  • persistence of hyaloid vascular system
    • only 1 out of 15 mice on a BALB/cJ background exhibit eye abnormalities (persistent vitreous vessels) compared to multiple and variable eye abnormalities seen on a C57BL/6J background   (MGI Ref ID J:82877)

Bmp4tm1Blh/Bmp4+

        involves: 129S2/SvPas * 129S6/SvEvTac * Black Swiss
  • behavior/neurological phenotype
  • *normal* behavior/neurological phenotype
    • mice do not display circling behavior on Black Swiss background but a small percentage do on a C57BL/6 background   (MGI Ref ID J:136636)

Bmp4tm1Blh/Bmp4+

        involves: 129S2/SvPas * C57BL/6
  • growth/size/body phenotype
  • decreased body weight
    • diabetic mutants (generated by treatment with streptozotocin, STZ) exhibit decreased body weight compared to untreated controls   (MGI Ref ID J:173500)
  • hearing/vestibular/ear phenotype
  • *normal* hearing/vestibular/ear phenotype
    • mice exhibit normal hearing   (MGI Ref ID J:159227)
    • abnormal ear development
      • at E12, mice exhibit small or absent lateral plate epithelium compared with wild-type mice with the central part least affected of all the parts   (MGI Ref ID J:159227)
    • abnormal lateral semicircular canal morphology
      • higher in the left ear than the right ear   (MGI Ref ID J:159227)
      • 70% of females and 62% of males on a mixed 129S2/SvPas and C57BL/6 background exhibit abnormal semicircular canal compared to only 9% of mice on a mixed 129S2/SvPas, C57BL/6, and CBA background   (MGI Ref ID J:159227)
      • absent lateral semicircular canal
        • the lateral duct and cartilage lining are absent in some mice   (MGI Ref ID J:159227)
      • decreased lateral semicircular canal size
        • partially truncated, constricted, or absent in some mice   (MGI Ref ID J:159227)
  • behavior/neurological phenotype
  • circling
    • in 37% of females and 24% of males strongly associated with bilateral defects in the lateral semicircular canal   (MGI Ref ID J:159227)
  • homeostasis/metabolism phenotype
  • increased circulating glucose level
    • diabetic mutants (generated by treatment with streptozotocin, STZ) have increased blood glucose similar to STZ-treated wild-type mice   (MGI Ref ID J:173500)
  • renal/urinary system phenotype
  • abnormal renal glomerulus morphology
    • mice show attenuation of mesangial expansion compare to diabetic (STZ-treated) wild-type   (MGI Ref ID J:173500)

Bmp4tm1Blh/Bmp4tm1Blh

        involves: 129S2/SvPas * Black Swiss
  • mortality/aging
  • complete embryonic lethality during organogenesis
  • growth/size/body phenotype
  • embryonic growth retardation   (MGI Ref ID J:53311)
    • at E10.5, homozygotes have 20-27 somites, while littermates have greater than 35   (MGI Ref ID J:51570)
  • vision/eye phenotype
  • abnormal lens induction
    • failure of lens placode induction   (MGI Ref ID J:51570)
  • embryogenesis phenotype
  • absent allantois
    • all embryos lacked an allantois   (MGI Ref ID J:53311)
  • embryonic growth retardation   (MGI Ref ID J:53311)
    • at E10.5, homozygotes have 20-27 somites, while littermates have greater than 35   (MGI Ref ID J:51570)
  • reproductive system phenotype
  • absent primordial germ cells   (MGI Ref ID J:53311)
  • endocrine/exocrine gland phenotype
  • absent Rathke's pouch
    • no sign of Rathke's pouch formation (either ectodermal thickening or invagination) is noted at E9.5-E9.75   (MGI Ref ID J:50517)
  • nervous system phenotype
  • absent Rathke's pouch
    • no sign of Rathke's pouch formation (either ectodermal thickening or invagination) is noted at E9.5-E9.75   (MGI Ref ID J:50517)

Bmp4tm1Blh/Bmp4tm1Blh

        involves: 129S2/SvPas * C57BL/6
  • mortality/aging
  • complete embryonic lethality
    • animals die between E7.5 and E10.5; most are arrested at the egg cylinder stage   (MGI Ref ID J:28717)
  • embryogenesis phenotype
  • abnormal extraembryonic mesoderm development
    • absent extraembryonic mesoderm   (MGI Ref ID J:28717)
  • abnormal rostral-caudal axis patterning
    • posterior patterning abnormalities   (MGI Ref ID J:28717)
  • abnormal visceral yolk sac morphology
    • visceral yolk sac often have a "blebby" appearance attributable to the paucity of extraembryonic mesoderm and blood islands underlying the endoderm layer   (MGI Ref ID J:28717)
    • absent visceral yolk sac blood islands
      • embryos that develop past the egg cylinder stage have a paucity of blood islands   (MGI Ref ID J:28717)
  • embryonic growth retardation
    • embryos are grossly retarded but have undergone turning, have a beating heart and forelimb buds   (MGI Ref ID J:28717)
  • growth/size/body phenotype
  • embryonic growth retardation
    • embryos are grossly retarded but have undergone turning, have a beating heart and forelimb buds   (MGI Ref ID J:28717)

Bmp4tm1Blh/Bmp4tm1Blh

        B6.129S2-Bmp4tm1Blh/J
  • mortality/aging
  • complete embryonic lethality between implantation and somite formation
    • none survive beyond the egg cylinder stage   (MGI Ref ID J:28717)
  • embryogenesis phenotype
  • abnormal extraembryonic mesoderm development
    • hypoplastic extraembryonic mesoderm: small amount can be distinguished   (MGI Ref ID J:28717)
  • abnormal visceral yolk sac morphology   (MGI Ref ID J:28717)
    • absent visceral yolk sac blood islands
      • there is a relative paucity of blood islands containing red blood cells in the visceral yolk sac   (MGI Ref ID J:28717)
  • failure of primitive streak formation
    • absence of organized primitive streak   (MGI Ref ID J:28717)
  • hematopoietic system phenotype
  • decreased erythrocyte cell number
    • few red blood cells are found in the heart, dorsal aorta, and vessels of an E8.5 embryo   (MGI Ref ID J:28717)

Bmp4tm1Blh/Bmp4tm1Blh

        129S2/SvPas-Bmp4tm1Blh
  • mortality/aging
  • complete embryonic lethality between implantation and somite formation
    • none survive beyond the egg cylinder stage   (MGI Ref ID J:28717)
  • embryogenesis phenotype
  • abnormal extraembryonic mesoderm development
    • hypoplastic extraembryonic mesoderm: small amount can be distinguished   (MGI Ref ID J:28717)
  • abnormal visceral yolk sac morphology   (MGI Ref ID J:28717)
    • absent visceral yolk sac blood islands   (MGI Ref ID J:28717)
  • failure of primitive streak formation
    • absence of organized primitive streak   (MGI Ref ID J:28717)

Bmp4tm1Blh/Bmp4tm1Blh

        involves: 129S2/SvPas * ICR
  • mortality/aging
  • complete embryonic lethality during organogenesis
    • embryos die between E9.5-E11.5   (MGI Ref ID J:28717)
  • growth/size/body phenotype
  • embryonic growth retardation
    • are grossly retarded but have undergone turning, have a beating heart and forelimb buds   (MGI Ref ID J:28717)
  • embryogenesis phenotype
  • embryonic growth retardation
    • are grossly retarded but have undergone turning, have a beating heart and forelimb buds   (MGI Ref ID J:28717)

Bmp4tm1Blh/Bmp4tm1Blh

        involves: 129S2/SvPas * C57BL/6 * CBA
  • mortality/aging
  • complete prenatal lethality   (MGI Ref ID J:53311)
  • embryogenesis phenotype
  • absent allantois
    • all embryos lacked an allantois   (MGI Ref ID J:53311)
  • embryonic growth retardation   (MGI Ref ID J:53311)
  • reproductive system phenotype
  • absent primordial germ cells
    • absent primordial germ cells   (MGI Ref ID J:53311)
  • growth/size/body phenotype
  • embryonic growth retardation   (MGI Ref ID J:53311)

Bmp4tm1Blh/Bmp4tm1Blh

        involves: 129S2/SvPas * 129S6/SvEvTac * Black Swiss
  • embryogenesis phenotype
  • abnormal amniotic cavity morphology
    • much of the posterior amniotic cavity is filled up by a mass of mesodermal cells that extends to the apical surface of the ectoderm   (MGI Ref ID J:79391)
  • abnormal mesoderm development
    • a mass of mesodermal cells extending to the apical surface of the ectoderm fills much of the posterior amniotic cavity   (MGI Ref ID J:79391)
    • abnormal lateral plate mesoderm morphology
      • at the 2 to 8 somite stage, over 75% of embryos show patch or reduced Nodal expression and lack expression in the left lateral plate mesoderm   (MGI Ref ID J:79391)
      • in about 65% of embryos at the 3 to 4 somite stage Lefty2 expression is absent from the lateral plate mesoderm   (MGI Ref ID J:79391)
  • abnormal posterior primitive streak morphology
    • an abnormal posterior bulge is present from the headfold to 6 somite stage   (MGI Ref ID J:79391)
  • abnormal primitive node morphology
    • node is either flat or slightly convex with an irregular periphery in embryos from the headfold to 6 somite stage unlike in controls where it is concave   (MGI Ref ID J:79391)
    • one embryo had large endoderm-like cells within the node   (MGI Ref ID J:79391)
  • cardiovascular system phenotype
  • abnormal heart looping
    • at the 9 to 10 somite stage in about 50% of embryos the heart tube lies centrally along the midline and shows no signs of looping   (MGI Ref ID J:79391)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Cancer Research
Genes Regulating Growth and Proliferation
Growth Factors/Receptors/Cytokines

Dermatology Research
Skin and Hair Texture Defects

Developmental Biology Research
Craniofacial and Palate Defects
Embryonic Lethality (Homozygous)
Eye Defects
Growth Defects
Internal/Organ Defects
      kidney
Skeletal Defects

Immunology, Inflammation and Autoimmunity Research
Growth Factors/Receptors/Cytokines

Internal/Organ Research
Kidney Defects

Reproductive Biology Research
Developmental Defects Affecting Gonads
Fertility Defects

Sensorineural Research
Eye Defects

Bmp4tm1Blh related

Cancer Research
Genes Regulating Growth and Proliferation
Growth Factors/Receptors/Cytokines

Dermatology Research
Skin and Hair Texture Defects

Developmental Biology Research
Craniofacial and Palate Defects
Embryonic Lethality (Homozygous)
Eye Defects
Growth Defects
Internal/Organ Defects
      kidney
Skeletal Defects

Endocrine Deficiency Research
Bone/Bone Marrow Defects
Gonad Defects
Kidney Defects

Immunology, Inflammation and Autoimmunity Research
Growth Factors/Receptors/Cytokines

Internal/Organ Research
Kidney Defects

Reproductive Biology Research
Developmental Defects Affecting Gonads
Fertility Defects

Sensorineural Research
Eye Defects

Pde6b+ related
Retinal Degeneration
      wild-type

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Bmp4tm1Blh
Allele Name targeted mutation 1, Brigid L Hogan
Allele Type Targeted (Null/Knockout)
Common Name(s) Bmp-4tm1blh; Bmp4-; Bmp4delta; Bmp4tm1;
Mutation Made By Brigid Hogan,   Duke University Medical Center
Strain of Origin129S2/SvPas
ES Cell Line NameD3
ES Cell Line Strain129S2/SvPas
Gene Symbol and Name Bmp4, bone morphogenetic protein 4
Chromosome 14
Gene Common Name(s) BMP2B; BMP2B1; BOMPR4A; Bmp-4; Bmp2b; Bmp2b-1; Bmp2b1; MCOPS6; OFC11; ZYME; bone morphogenetic protein 2b; bone morphogenetic protein 2b 1;
Molecular Note A neomycin selection cassette replaced a genomic fragment containing all of the coding sequence of the gene after the seventh codon of exon 1. An oligonucleotide encoding a stop codon in all three reading frames was also inserted into exon 2. [MGI Ref ID J:28717]
 
Allele Symbol Pde6b+
Allele Name wild type
Allele Type Not Applicable
Mutation Made By Frank Kooy,   University of Antwerp
Gene Symbol and Name Pde6b, phosphodiesterase 6B, cGMP, rod receptor, beta polypeptide
Chromosome 5
Gene Common Name(s) CSNB3; CSNBAD2; PDEB; Pdeb; RP40; nmf137; phosphodiesterase, cGMP, rod receptor, beta polypeptide; r; rd; rd-1; rd1; rd10; retinal degeneration; retinal degeneration 1; retinal degeneration 10;

Genotyping

Genotyping Information

Genotyping Protocols

Bmp4tm1Blh, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Winnier G; Blessing M; Labosky PA; Hogan BL. 1995. Bone morphogenetic protein-4 is required for mesoderm formation and patterning in the mouse. Genes Dev 9(17):2105-16. [PubMed: 7657163]  [MGI Ref ID J:28717]

Additional References

Bmp4tm1Blh related

Blauwkamp MN; Beyer LA; Kabara L; Takemura K; Buck T; King WM; Dolan DF; Barald KF; Raphael Y; Koenig RJ. 2007. The role of bone morphogenetic protein 4 in inner ear development and function. Hear Res 225(1-2):71-9. [PubMed: 17275231]  [MGI Ref ID J:118380]

Chang B; Hawes NL; Hurd RE; Wang J; Howell D; Davisson MT; Roderick TH; Nusinowitz S; Heckenlively JR. 2005. Mouse models of ocular diseases. Vis Neurosci 22(5):587-93. [PubMed: 16332269]  [MGI Ref ID J:156373]

Chang B; Smith RS; Peters M; Savinova OV; Hawes NL; Zabaleta A; Nusinowitz S; Martin JE; Davisson ML; Cepko CL; Hogan BL; John SW. 2001. Haploinsufficient Bmp4 ocular phenotypes include anterior segment dysgenesis with elevated intraocular pressure. BMC Genet 2(1):18. [PubMed: 11722794]  [MGI Ref ID J:82877]

Chang W; Lin Z; Kulessa H; Hebert J; Hogan BL; Wu DK. 2008. Bmp4 is essential for the formation of the vestibular apparatus that detects angular head movements. PLoS Genet 4(4):e1000050. [PubMed: 18404215]  [MGI Ref ID J:136636]

Christ A; Christa A; Kur E; Lioubinski O; Bachmann S; Willnow TE; Hammes A. 2012. LRP2 Is an Auxiliary SHH Receptor Required to Condition the Forebrain Ventral Midline for Inductive Signals. Dev Cell 22(2):268-78. [PubMed: 22340494]  [MGI Ref ID J:181474]

Dunn NR; Winnier GE; Hargett LK; Schrick JJ; Fogo AB; Hogan BL. 1997. Haploinsufficient phenotypes in Bmp4 heterozygous null mice and modification by mutations in Gli3 and Alx4. Dev Biol 188(2):235-47. [PubMed: 9268572]  [MGI Ref ID J:42445]

Fujiwara T; Dehart DB; Sulik KK; Hogan BL. 2002. Distinct requirements for extra-embryonic and embryonic bone morphogenetic protein 4 in the formation of the node and primitive streak and coordination of left-right asymmetry in the mouse. Development 129(20):4685-96. [PubMed: 12361961]  [MGI Ref ID J:79391]

Fujiwara T; Dunn NR; Hogan BL. 2001. Bone morphogenetic protein 4 in the extraembryonic mesoderm is required for allantois development and the localization and survival of primordial germ cells in the mouse. Proc Natl Acad Sci U S A 98(24):13739-44. [PubMed: 11707591]  [MGI Ref ID J:72979]

Furuta Y; Hogan BLM. 1998. BMP4 is essential for lens induction in the mouse embryo. Genes Dev 12(23):3764-75. [PubMed: 9851982]  [MGI Ref ID J:51570]

Goncalves A; Zeller R. 2011. Genetic Analysis Reveals an Unexpected Role of BMP7 in Initiation of Ureteric Bud Outgrowth in Mouse Embryos. PLoS One 6(4):e19370. [PubMed: 21552539]  [MGI Ref ID J:172359]

Hu J; Chen YX; Wang D; Qi X; Li TG; Hao J; Mishina Y; Garbers DL; Zhao GQ. 2004. Developmental expression and function of Bmp4 in spermatogenesis and in maintaining epididymal integrity. Dev Biol 276(1):158-71. [PubMed: 15531371]  [MGI Ref ID J:95022]

Jia S; Zhou J; Gao Y; Baek JA; Martin JF; Lan Y; Jiang R. 2013. Roles of Bmp4 during tooth morphogenesis and sequential tooth formation. Development 140(2):423-32. [PubMed: 23250216]  [MGI Ref ID J:191714]

Jiao K; Kulessa H; Tompkins K; Zhou Y; Batts L; Baldwin HS; Hogan BL. 2003. An essential role of Bmp4 in the atrioventricular septation of the mouse heart. Genes Dev 17(19):2362-7. [PubMed: 12975322]  [MGI Ref ID J:86001]

Katagiri T; Boorla S; Frendo JL; Hogan BL; Karsenty G. 1998. Skeletal abnormalities in doubly heterozygous Bmp4 and Bmp7 mice. Dev Genet 22(4):340-8. [PubMed: 9664686]  [MGI Ref ID J:48538]

Kulessa H; Hogan BL. 2002. Generation of a loxP flanked bmp4(loxP-lacZ) allele marked by conditional lacZ expression. Genesis 32(2):66-8. [PubMed: 11857779]  [MGI Ref ID J:75136]

Lamm ML; Podlasek CA; Barnett DH; Lee J; Clemens JQ; Hebner CM; Bushman W. 2001. Mesenchymal factor bone morphogenetic protein 4 restricts ductal budding and branching morphogenesis in the developing prostate. Dev Biol 232(2):301-14. [PubMed: 11401393]  [MGI Ref ID J:69382]

Lawson KA; Dunn NR; Roelen BA; Zeinstra LM; Davis AM; Wright CV ; Korving JP ; Hogan BL. 1999. Bmp4 is required for the generation of primordial germ cells in the mouse embryo [see comments] Genes Dev 13(4):424-36. [PubMed: 10049358]  [MGI Ref ID J:53311]

Lopez-Rios J; Speziale D; Robay D; Scotti M; Osterwalder M; Nusspaumer G; Galli A; Hollander GA; Kmita M; Zeller R. 2012. GLI3 constrains digit number by controlling both progenitor proliferation and BMP-dependent exit to chondrogenesis. Dev Cell 22(4):837-48. [PubMed: 22465667]  [MGI Ref ID J:184012]

Miura S; Mishina Y. 2011. Hepatocyte growth factor-regulated tyrosine kinase substrate (Hgs) is involved in BMP signaling through phosphorylation of smads and TAK1 in early mouse embryo. Dev Dyn 240(11):2474-81. [PubMed: 21953618]  [MGI Ref ID J:177114]

Murali D; Yoshikawa S; Corrigan RR; Plas DJ; Crair MC; Oliver G; Lyons KM; Mishina Y; Furuta Y. 2005. Distinct developmental programs require different levels of Bmp signaling during mouse retinal development. Development 132(5):913-23. [PubMed: 15673568]  [MGI Ref ID J:96964]

Ohinata Y; Ohta H; Shigeta M; Yamanaka K; Wakayama T; Saitou M. 2009. A signaling principle for the specification of the germ cell lineage in mice. Cell 137(3):571-84. [PubMed: 19410550]  [MGI Ref ID J:148768]

Paine-Saunders S; Viviano BL; Zupicich J; Skarnes WC; Saunders S. 2000. glypican-3 controls cellular responses to Bmp4 in limb patterning and skeletal development. Dev Biol 225(1):179-87. [PubMed: 10964473]  [MGI Ref ID J:64330]

Pangas SA. 2012. Regulation of the ovarian reserve by members of the transforming growth factor beta family. Mol Reprod Dev 79(10):666-79. [PubMed: 22847922]  [MGI Ref ID J:190579]

Takuma N; Sheng HZ; Furuta Y; Ward JM; Sharma K; Hogan BL; Pfaff SL; Westphal H; Kimura S; Mahon KA. 1998. Formation of Rathke's pouch requires dual induction from the diencephalon. Development 125(23):4835-40. [PubMed: 9806931]  [MGI Ref ID J:50517]

Tanaka SS; Nakane A; Yamaguchi YL; Terabayashi T; Abe T; Nakao K; Asashima M; Steiner KA; Tam PP; Nishinakamura R. 2013. Dullard/Ctdnep1 modulates WNT signalling activity for the formation of primordial germ cells in the mouse embryo. PLoS One 8(3):e57428. [PubMed: 23469192]  [MGI Ref ID J:199855]

Tominaga T; Abe H; Ueda O; Goto C; Nakahara K; Murakami T; Matsubara T; Mima A; Nagai K; Araoka T; Kishi S; Fukushima N; Jishage K; Doi T. 2011. Activation of bone morphogenetic protein 4 signaling leads to glomerulosclerosis that mimics diabetic nephropathy. J Biol Chem 286(22):20109-16. [PubMed: 21471216]  [MGI Ref ID J:173500]

Uchimura T; Komatsu Y; Tanaka M; McCann KL; Mishina Y. 2009. Bmp2 and Bmp4 genetically interact to support multiple aspects of mouse development including functional heart development. Genesis 47(6):374-84. [PubMed: 19391114]  [MGI Ref ID J:149892]

Vervoort R; Ceulemans H; Van Aerschot L; D'Hooge R; David G. 2010. Genetic modification of the inner ear lateral semicircular canal phenotype of the Bmp4 haplo-insufficient mouse. Biochem Biophys Res Commun 394(3):780-5. [PubMed: 20233579]  [MGI Ref ID J:159227]

Wijgerde M; Karp S; McMahon J; McMahon AP. 2005. Noggin antagonism of BMP4 signaling controls development of the axial skeleton in the mouse. Dev Biol 286(1):149-57. [PubMed: 16122729]  [MGI Ref ID J:103548]

Yamaji M; Seki Y; Kurimoto K; Yabuta Y; Yuasa M; Shigeta M; Yamanaka K; Ohinata Y; Saitou M. 2008. Critical function of Prdm14 for the establishment of the germ cell lineage in mice. Nat Genet 40(8):1016-22. [PubMed: 18622394]  [MGI Ref ID J:138571]

Ying Y; Liu XM; Marble A; Lawson KA; Zhao GQ. 2000. Requirement of Bmp8b for the generation of primordial germ cells in the mouse Mol Endocrinol 14(7):1053-63. [PubMed: 10894154]  [MGI Ref ID J:63160]

Ying Y; Zhao GQ. 2001. Cooperation of endoderm-derived BMP2 and extraembryonic ectoderm-derived BMP4 in primordial germ cell generation in the mouse. Dev Biol 232(2):484-92. [PubMed: 11401407]  [MGI Ref ID J:69376]

Zakin L; De Robertis EM. 2004. Inactivation of mouse Twisted gastrulation reveals its role in promoting Bmp4 activity during forebrain development. Development 131(2):413-24. [PubMed: 14681194]  [MGI Ref ID J:90398]

de Sousa Lopes SM; Roelen BA; Monteiro RM; Emmens R; Lin HY; Li E; Lawson KA; Mummery CL. 2004. BMP signaling mediated by ALK2 in the visceral endoderm is necessary for the generation of primordial germ cells in the mouse embryo. Genes Dev 18(15):1838-49. [PubMed: 15289457]  [MGI Ref ID J:91660]

Pde6b+ related

Dobkin C; Rabe A; Dumas R; El Idrissi A; Haubenstock H; Brown WT. 2000. Fmr1 knockout mouse has a distinctive strain-specific learning impairment. Neuroscience 100(2):423-9. [PubMed: 11008180]  [MGI Ref ID J:119166]

Ivanco TL; Greenough WT. 2002. Altered mossy fiber distributions in adult Fmr1 (FVB) knockout mice. Hippocampus 12(1):47-54. [PubMed: 11918288]  [MGI Ref ID J:113177]

Sakamoto K; McCluskey M; Wensel TG; Naggert JK; Nishina PM. 2009. New mouse models for recessive retinitis pigmentosa caused by mutations in the Pde6a gene. Hum Mol Genet 18(1):178-92. [PubMed: 18849587]  [MGI Ref ID J:142108]

Zhao MG; Toyoda H; Ko SW; Ding HK; Wu LJ; Zhuo M. 2005. Deficits in trace fear memory and long-term potentiation in a mouse model for fragile X syndrome. J Neurosci 25(32):7385-92. [PubMed: 16093389]  [MGI Ref ID J:100197]

Health & husbandry

Health & Colony Maintenance Information

Colony Maintenance

Breeding & HusbandryHeterozygotes are viable and fertile. Females are reported to breed best as close to 6 weeks of age as possible.
Mating SystemWild-type x Heterozygote         (Female x Male)   15-MAY-14
Heterozygote x Wild-type         (Female x Male)   15-MAY-14

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


 

This strain is currently Under Development - Now Accepting Orders.
Estimated Available for Distribution Date: 16-MAR-15

Please note: You may now place orders for this strain although it is not yet ready for distribution. Estimated available for distribution dates are provided to keep customers better informed on strains under development. Please note that our Colony Managers routinely monitor the target date and edit it based on breeding performance and other factors. The length of time it takes to make a new strain available for distribution depends on genotype, age, number of animals sent by the Donating Investigator, breeding performance, additional strain development (backcrossing, making homozygous), and anticipated demand for the strain.

Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $232.00Female or MaleWild-type for Pde6b+, Heterozygous for Bmp4tm1Blh  
Price per Pair (US dollars $)Pair Genotype
$302.00Wild-type for Pde6b<+> Heterozygous for Bmp4 x Wild-type for Pde6b<+> Wild-type for Bmp4  
$302.00Wild-type for Pde6b<+> Wild-type for Bmp4 x Wild-type for Pde6b<+> Heterozygous for Bmp4  

Standard Supply

Under Development - Now Accepting Orders The strain development process (i.e. importation, rederivation, and colony expansion) usually takes six to nine months.

Cryopreserved

Frozen Products

Price (US dollars $)
Frozen Embryo $1650.00

Standard Supply

Under Development - Now Accepting Orders The strain development process (i.e. importation, rederivation, and colony expansion) usually takes six to nine months.

Supply Notes

  • Cryopreserved Embryos
    Available to most shipping destinations1
    This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.

    1 Shipments cannot be made to Australia due to Australian government import restrictions.
    2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.
Pricing for International shipping destinations View USA Canada and Mexico Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $301.60Female or MaleWild-type for Pde6b+, Heterozygous for Bmp4tm1Blh  
Price per Pair (US dollars $)Pair Genotype
$392.60Wild-type for Pde6b<+> Heterozygous for Bmp4 x Wild-type for Pde6b<+> Wild-type for Bmp4  
$392.60Wild-type for Pde6b<+> Wild-type for Bmp4 x Wild-type for Pde6b<+> Heterozygous for Bmp4  

Standard Supply

Under Development - Now Accepting Orders The strain development process (i.e. importation, rederivation, and colony expansion) usually takes six to nine months.

Cryopreserved

Frozen Products

Price (US dollars $)
Frozen Embryo $2145.00

Standard Supply

Under Development - Now Accepting Orders The strain development process (i.e. importation, rederivation, and colony expansion) usually takes six to nine months.

Supply Notes

  • Cryopreserved Embryos
    Available to most shipping destinations1
    This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.

    1 Shipments cannot be made to Australia due to Australian government import restrictions.
    2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.
View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Under Development - Now Accepting Orders The strain development process (i.e. importation, rederivation, and colony expansion) usually takes six to nine months.

Control Information

  Control
   Wild-type from the colony
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.


See Terms of Use tab for General Terms and Conditions


The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

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