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Under Development - Now Accepting Orders

Estimated Available for Distribution Date: 16-MAR-15
These Fa2hflox/flox mice may be useful for studying the lipid profile of myelin disorders of the central nervous system.


Strain Information

Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Mating SystemHeterozygote x Heterozygote         (Female x Male)   26-AUG-14
Specieslaboratory mouse
GenerationN7+pN1 (31-OCT-14)
Generation Definitions

These Fa2hflox/flox mice possess loxP sites flanking exons 5-6 of the fatty acid 2-hydroxylase (Fa2h) gene. FA2H catalyzes the synthesis of 2-hydroxysphingolipids, a subset of sphingolipids that contain 2-hydroxy fatty acids. Mutations in the human gene have been associated with leukodystrophy, spastic paraplegia, and neurodegeneration with brain iron accumulation. Mice that are homozygous for this allele are viable and fertile. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exons 5-6 deleted in the cre-expressing tissues.

A targeting vector was designed to insert a loxP site upstream of exon 5 followed by a frt-flanked neomycin resistance (neo) cassette, and a second loxP site downstream of exon 6 of the fatty acid 2-hydroxylase (Fa2h) gene. The construct was electroporated into 129Sv/J embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and resulting chimeric mice were bred with Flpe transgenic mice on a B6 background to delete the neo cassette. Progeny were crossed to remove the Flp-expressing transgene. These mice were bred to C57BL/6NCrl mice for 3 generations and then to C57BL/6J for at least 7 generations. Upon arrival, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.

Control Information

   000664 C57BL/6J (approximate)
  Considerations for Choosing Controls


Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested.
Spastic Paraplegia 35, Autosomal Recessive; SPG35   (FA2H)
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.


        involves: 129
  • no phenotypic analysis
  • *normal* no phenotypic analysis   (MGI Ref ID J:204276)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Metabolism Research
Lipid Metabolism

Neurobiology Research
Cre-lox System
      loxP-flanked Sequences
Myelination Defects

Research Tools
Cre-lox System
      loxP-flanked Sequences

Genes & Alleles

Gene & Allele Information provided by MGI

Allele Symbol Fa2htm1.2Hama
Allele Name targeted mutation 1.2, Hiroko Hama
Allele Type Targeted (Conditional ready (e.g. floxed), No functional change)
Strain of Origin129
Gene Symbol and Name Fa2h, fatty acid 2-hydroxylase
Chromosome 8
Gene Common Name(s) FAAH; FAH1; FAXDC1; Faxdc1; G630055L08Rik; RGD1310347; RIKEN cDNA G630055L08 gene; SCS7; SPG35; Wdr59; fatty acid hydroxylase domain containing 1;
Molecular Note A loxP site was inserted in intron 4, and a Frt-flanked neomycin resistance cassette and a loxP site were inserted in intron 6. Flp-mediated recombination removed the neomycin resistance cassette and left exons 5 and 6 floxed. [MGI Ref ID J:204276]


Genotyping Information

Genotyping Protocols

Fa2htm1.2Hama-Alternate 1, Standard PCR

Helpful Links

Genotyping resources and troubleshooting


References provided by MGI

Selected Reference(s)

Potter KA; Kern MJ; Fullbright G; Bielawski J; Scherer SS; Yum SW; Li JJ; Cheng H; Han X; Venkata JK; Akbar Ali Khan P; Rohrer B; Hama H. 2011. Central nervous system dysfunction in a mouse model of Fa2h deficiency. Glia 59(7):1009-21. [PubMed: 21491498]  [MGI Ref ID J:171655]

Additional References

Fa2htm1.2Hama related

Hama H. 2014. Direct Data Submission MGI Direct Data Submission :.  [MGI Ref ID J:204276]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX12

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, homozygous mice may be bred together.
Mating SystemHeterozygote x Heterozygote         (Female x Male)   26-AUG-14
Diet Information New Diet as of April 7, 2014: Lab Diet® 5K0Q

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


This strain is currently Under Development - Now Accepting Orders.
Estimated Available for Distribution Date: 16-MAR-15

Please note: You may now place orders for this strain although it is not yet ready for distribution. Estimated available for distribution dates are provided to keep customers better informed on strains under development. Please note that our Colony Managers routinely monitor the target date and edit it based on breeding performance and other factors. The length of time it takes to make a new strain available for distribution depends on genotype, age, number of animals sent by the Donating Investigator, breeding performance, additional strain development (backcrossing, making homozygous), and anticipated demand for the strain.

Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $232.00Female or MaleHeterozygous for Fa2htm1.2Hama  
$232.00Female or MaleHomozygous for Fa2htm1.2Hama  
Price per Pair (US dollars $)Pair Genotype
$464.00Heterozygous for Fa2htm1.2Hama x Heterozygous for Fa2htm1.2Hama  

Standard Supply

Under Development - Now Accepting Orders The strain development process (i.e. importation, rederivation, and colony expansion) usually takes six to nine months.

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $301.60Female or MaleHeterozygous for Fa2htm1.2Hama  
$301.60Female or MaleHomozygous for Fa2htm1.2Hama  
Price per Pair (US dollars $)Pair Genotype
$603.20Heterozygous for Fa2htm1.2Hama x Heterozygous for Fa2htm1.2Hama  

Standard Supply

Under Development - Now Accepting Orders The strain development process (i.e. importation, rederivation, and colony expansion) usually takes six to nine months.

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Under Development - Now Accepting Orders The strain development process (i.e. importation, rederivation, and colony expansion) usually takes six to nine months.

Control Information

   000664 C57BL/6J (approximate)
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.

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Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

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The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

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