Strain Name:

B6.129(Cg)-Fa2htm1.2Hama/J

Stock Number:

024877

Availability:

Awaiting Transfer from the Donor

On 10-APR-14 this strain was accepted for import and is now awaiting transfer to our campus.
Use Restrictions Apply, see Terms of Use
Register Interest
These Fa2hflox/flox mice may be useful for studying the lipid profile of myelin disorders of the central nervous system.

Description

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Specieslaboratory mouse
 
Donating Investigator Hiroko Hama,   Medical University of South Carolina

Description
These Fa2hflox/flox mice possess loxP sites flanking exons 5-6 of the fatty acid 2-hydroxylase (Fa2h) gene. FA2H catalyzes the synthesis of 2-hydroxysphingolipids, a subset of sphingolipids that contain 2-hydroxy fatty acids. Mutations in the human gene have been associated with leukodystrophy, spastic paraplegia, and neurodegeneration with brain iron accumulation. Mice that are homozygous for this allele are viable and fertile. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exons 5-6 deleted in the cre-expressing tissues.

Development
A targeting vector was designed to insert a loxP site upstream of exon 5 followed by a frt-flanked neomycin resistance (neo) cassette, and a second loxP site downstream of exon 6 of the fatty acid 2-hydroxylase (Fa2h) gene. The construct was electroporated into 129Sv/J embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and resulting chimeric mice were bred with Flpe transgenic mice on a B6 background to delete the neo cassette. Progeny were crossed to remove the Flp-expressing transgene. These mice were bred to C57BL/6NCrl mice for 3 generations and then to C57BL/6J for at least 7 generations. Upon arrival, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.

Control Information

  Control
   000664 C57BL/6J (approximate)
 
  Considerations for Choosing Controls

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested.
Spastic Paraplegia 35, Autosomal Recessive; SPG35   (FA2H)
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Fa2htm1.2Hama/Fa2h+

        involves: 129
  • no phenotypic analysis
  • *normal* no phenotypic analysis   (MGI Ref ID J:204276)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Metabolism Research
Lipid Metabolism

Neurobiology Research
Cre-lox System
      loxP-flanked Sequences
Myelination Defects
Neurodegeneration

Research Tools
Cre-lox System
      loxP-flanked Sequences

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Fa2htm1.2Hama
Allele Name targeted mutation 1.2, Hiroko Hama
Allele Type Targeted (Conditional ready (e.g. floxed), No functional change)
Strain of Origin129
Gene Symbol and Name Fa2h, fatty acid 2-hydroxylase
Chromosome 8
Gene Common Name(s) FAAH; FAH1; FAXDC1; Faxdc1; G630055L08Rik; RGD1310347; RIKEN cDNA G630055L08 gene; SCS7; SPG35; Wdr59; fatty acid hydroxylase domain containing 1;
Molecular Note A loxP site was inserted in intron 4, and a Frt-flanked neomycin resistance cassette and a loxP site were inserted in intron 6. Flp-mediated recombination removed the neomycin resistance cassette and left exons 5 and 6 floxed. [MGI Ref ID J:204276]

Genotyping

Genotyping Information


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Potter KA; Kern MJ; Fullbright G; Bielawski J; Scherer SS; Yum SW; Li JJ; Cheng H; Han X; Venkata JK; Akbar Ali Khan P; Rohrer B; Hama H. 2011. Central nervous system dysfunction in a mouse model of Fa2h deficiency. Glia 59(7):1009-21. [PubMed: 21491498]  [MGI Ref ID J:171655]

Additional References

Fa2htm1.2Hama related

Hama H. 2014. Direct Data Submission MGI Direct Data Submission :.  [MGI Ref ID J:204276]

Health & husbandry

Health & Colony Maintenance Information

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, homozygous mice may be bred together.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


 

This strain is currently Awaiting Transfer from the Donor.

On 10-APR-14 this strain was accepted for import and is now awaiting transfer to our campus.

Register Interest

View All Strains Awaiting Transfer from the Donor, In Progress and On Hold

Control Information

  Control
   000664 C57BL/6J (approximate)
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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