Strain Name:

B6N.129P2-Cx3cr1tm3(DTR)Litt/J

Stock Number:

025629

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Availability:

Under Development - Now Accepting Orders

Estimated Available for Distribution Date: 05-JAN-15
Use Restrictions Apply, see Terms of Use
Cre excision of a floxed stop cassette in these Cx3cr1 knockin mice enables selective depletion of associated cells upon administration of diphtheria toxin.

Description

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
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Additional information on Congenic nomenclature.
Mating SystemHeterozygote x Hemizygote         (Female x Male)   26-AUG-14
Specieslaboratory mouse
GenerationN16+pN1 (10-NOV-14)
Generation Definitions
 
Donating InvestigatorDr. Dan R. Littman,   New York University Medical Center

Description
CDC11c+ mononuclear phagocytes expressing high levels of Cx3cr1 (chemokine (C-X3-C motif) receptor 1) have been found to traffic bacteria from the gut to mesenteric lymph nodes (MLNs) where T-cell responses and IgA production are induced.

A loxP-flanked stop cassette upstream of the diptheria toxin receptor (DTR)-coding region was knocked into exon 1 of the mouse Cx3cr1 locus. Expression of CD11c-Cre (e.g. through crosses with Stock No. 008068) excises the stop cassette, allowing for DTR expression and selective depletion of CD11c+CX3CR1hi cells upon administration of diphtheria toxin, with no effect on CD11c+ CX3CR1int or CD11c+CD103+cells. When mice are orally infected with non-invasive Salmonella after diptheria-toxin-mediated depletion of the CX3CR1hi mononuclear cells, there is a marked reduction in the number of bacteria reaching the mesenteric lymph nodes. Although YFP is incorporated in the knock-in construct, expression is not observed.

Development
A loxP-Stop-loxP-diptheria toxin receptor (DTR)-IRES-YFP cassette was knocked into exon 1 of the gene through homologous recombination in 129P2/OlaHsd-derived E14 embryonic stem (ES) cells. Resultant mice were backcrossed to C57BL/6NTac for 16 generations by the donating laboratory.

Control Information

  Control
   Wild-type from the colony
   005304 C57BL/6NJ
 
  Considerations for Choosing Controls

Related Strains

View Strains carrying other alleles of Cx3cr1     (5 strains)

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested.
Coronary Heart Disease, Susceptibility to, 1   (CX3CR1)
Human Immunodeficiency Virus Type 1, Susceptibility to   (CX3CR1)
Macular Degeneration, Age-Related, 12; ARMD12   (CX3CR1)
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype relates to a compound genotype created using this strain.
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Cx3cr1tm3(DTR)Litt/Cx3cr1tm3(DTR)Litt Tg(Itgax-cre)1-1Reiz/?

        involves: 129P2/OlaHsd * C57BL/6 * CBA
  • immune system phenotype
  • decreased susceptibility to bacterial infection
    • mice treated with diphtheria toxin (DTR) and infected with non-invasive Salmonella exhibit decreased bacterial titers in the mesenteric lymph nodes as compared to mice carrying Cx3cr1tm3(DTR)Litt in the absence of cre recombinase   (MGI Ref ID J:210086)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Research Tools
Cre-lox System
      loxP-flanked Sequences
Immunology, Inflammation and Autoimmunity Research
      genes regulating susceptibility to infectious disease and endotoxin

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Cx3cr1tm3(DTR)Litt
Allele Name targeted mutation 3, Dan R Littman
Allele Type Targeted (Conditional ready (e.g. floxed), No functional change)
Common Name(s) CX3CR1-STOP-DTR;
Strain of Origin129P2/OlaHsd
Gene Symbol and Name Cx3cr1, chemokine (C-X3-C motif) receptor 1
Chromosome 9
Gene Common Name(s) CCRL1; CMKBRL1; CMKDR1; GPR13; GPRV28; Rbs11; V28;
Molecular Note The targeting vector containing a loxP-Stop-loxP-diptheria toxin receptor (DTR)-IRES-YFP cassette was inserted into exon 1. [MGI Ref ID J:101977] [MGI Ref ID J:210086]

Genotyping

Genotyping Information

Genotyping Protocols

Cx3cr1tm3(DTR)Litt, Standard PCR
Generic Cre Melt Curve Analysis,

MELT



Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Diehl GE; Longman RS; Zhang JX; Breart B; Galan C; Cuesta A; Schwab SR; Littman DR. 2013. Microbiota restricts trafficking of bacteria to mesenteric lymph nodes by CX(3)CR1(hi) cells. Nature 494(7435):116-20. [PubMed: 23334413]  [MGI Ref ID J:210086]

Additional References

Cx3cr1tm3(DTR)Litt related

The Jackson Laboratory. 2005. Information obtained from The Jackson Laboratory, Bar Harbor, ME Unpublished :.  [MGI Ref ID J:101977]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX12

Colony Maintenance

Breeding & HusbandryHeterozygotes and homozygotes are viable and fertile.
Mating SystemHeterozygote x Hemizygote         (Female x Male)   26-AUG-14
Diet Information New Diet as of April 7, 2014: Lab Diet® 5K0Q

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


 

This strain is currently Under Development - Now Accepting Orders.
Estimated Available for Distribution Date: 05-JAN-15

Please note: You may now place orders for this strain although it is not yet ready for distribution. Estimated available for distribution dates are provided to keep customers better informed on strains under development. Please note that our Colony Managers routinely monitor the target date and edit it based on breeding performance and other factors. The length of time it takes to make a new strain available for distribution depends on genotype, age, number of animals sent by the Donating Investigator, breeding performance, additional strain development (backcrossing, making homozygous), and anticipated demand for the strain.

Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $232.00Female or MaleHeterozygous for Cx3cr1tm3(DTR)Litt  
$232.00FemaleHomozygous for Cx3cr1tm3(DTR)Litt  
Price per Pair (US dollars $)Pair Genotype
$464.00Heterozygous for Cx3cr1tm3(DTR)Litt x Heterozygous for Cx3cr1tm3(DTR)Litt  

Standard Supply

Under Development - Now Accepting Orders The strain development process (i.e. importation, rederivation, and colony expansion) usually takes six to nine months.

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $301.60Female or MaleHeterozygous for Cx3cr1tm3(DTR)Litt  
$301.60FemaleHomozygous for Cx3cr1tm3(DTR)Litt  
Price per Pair (US dollars $)Pair Genotype
$603.20Heterozygous for Cx3cr1tm3(DTR)Litt x Heterozygous for Cx3cr1tm3(DTR)Litt  

Standard Supply

Under Development - Now Accepting Orders The strain development process (i.e. importation, rederivation, and colony expansion) usually takes six to nine months.

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Under Development - Now Accepting Orders The strain development process (i.e. importation, rederivation, and colony expansion) usually takes six to nine months.

Control Information

  Control
   Wild-type from the colony
   005304 C57BL/6NJ
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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