Genotyping resources & troubleshooting

Genotyping protocols for JAX® Mice are provided as a starting point for developing your own optimized assay. The resources below are provided to assist you.

PCR genotyping tips and trouble-shooting

Great tips for trouble-shooting the most common genotyping problems

DNA isolation protocols

Commonly used DNA isolation methods

General PCR reminders

Helpful reminders for PCR genotyping

PCR resources - online links, references, & books

Valuable PCR genotyping information from other sources

Frequently asked genotyping questions

The most common PCR genotyping questions and answers

Resources for non-standard genotyping

Information about qPCR, melting curve analysis, pyrosequencing and lacZ staining

PCR protocols for JAX® Mice

  • PCR protocols, provided as a starting point to develop your own assay, are available on the 'Genotyping' tab of JAX® Mice strain data sheets. Find the data sheet for your strain by using the JAX® Mice Database quick query form.
  • Search an index of all available PCR genotyping assays for JAX® Mice by stock# or current gene symbol through the Genotyping Protocol Database.

Control DNA available

Order highly-purified mutant and/or control DNA from the Mouse DNA Resource.